Early Appearance of Nonvisual and Circadian Markers in the Developing Inner Retinal Cells of Chicken

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2014 Jul 1

PMID 24977155

Citations 5

Authors

Nicolas M Diaz

Luis P Morera

Daniela M Verra

Maria A Contin

Mario E Guido

Affiliations

Soon will be listed here.

Abstract

The retina is a key component of the vertebrate circadian system; it is responsible for detecting and transmitting the environmental illumination conditions (day/night cycles) to the brain that synchronize the circadian clock located in the suprachiasmatic nucleus (SCN). For this, retinal ganglion cells (RGCs) project to the SCN and other nonvisual areas. In the chicken, intrinsically photosensitive RGCs (ipRGCs) expressing the photopigment melanopsin (Opn4) transmit photic information and regulate diverse nonvisual tasks. In nonmammalian vertebrates, two genes encode Opn4: the Xenopus (Opn4x) and the mammalian (Opn4m) orthologs. RGCs express both Opn4 genes but are not the only inner retinal cells expressing Opn4x: horizontal cells (HCs) also do so. Here, we further characterize primary cultures of both populations of inner retinal cells (RGCs and HCs) expressing Opn4x. The expression of this nonvisual photopigment, as well as that for different circadian markers such as the clock genes Bmal1, Clock, Per2, and Cry1, and the key melatonin synthesizing enzyme, arylalkylamine N-acetyltransferase (AA-NAT), appears very early in development in both cell populations. The results clearly suggest that nonvisual Opn4 photoreceptors and endogenous clocks converge all together in these inner retinal cells at early developmental stages.

Citing Articles

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Melanopsin phototransduction: beyond canonical cascades.

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Non-visual Opsins and Novel Photo-Detectors in the Vertebrate Inner Retina Mediate Light Responses Within the Blue Spectrum Region.

Guido M, Marchese N, Rios M, Morera L, Diaz N, Garbarino-Pico E Cell Mol Neurobiol. 2020; 42(1):59-83.

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Expression of Non-visual Opsins Opn3 and Opn5 in the Developing Inner Retinal Cells of Birds. Light-Responses in Müller Glial Cells.

Rios M, Marchese N, Guido M Front Cell Neurosci. 2019; 13:376.

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The Visual Cycle in the Inner Retina of Chicken and the Involvement of Retinal G-Protein-Coupled Receptor (RGR).

Diaz N, Morera L, Tempesti T, Guido M Mol Neurobiol. 2016; 54(4):2507-2517.

PMID: 26984602 DOI: 10.1007/s12035-016-9830-5.

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