Bradyrhizobium Ottawaense Sp. Nov., a Symbiotic Nitrogen Fixing Bacterium from Root Nodules of Soybeans in Canada

Overview

Journal Int J Syst Evol Microbiol

Specialty Microbiology

Date 2014 Jun 28

PMID 24969302

Citations 23

Authors

Xiumei Yu

Sylvie Cloutier

James T Tambong

Eden S P Bromfield

Affiliations

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Abstract

Sixteen strains of symbiotic bacteria from root nodules of Glycine max grown in Ottawa, Canada, were previously characterized and placed in a novel group within the genus Bradyrhizobium. To verify their taxonomic status, these strains were further characterized using a polyphasic approach. All strains possessed identical 16S rRNA gene sequences that were 99.79 % similar to the closest relative, Bradyrhizobium liaoningense LMG 18230(T). Phylogenetic analysis of concatenated atpD, glnII, recA, gyrB, rpoB and dnaK genes divided the 16 strains into three multilocus sequence types that were placed in a highly supported lineage distinct from named species of the genus Bradyrhizobium consistent with results of DNA-DNA hybridization. Based on analysis of symbiosis gene sequences (nodC and nifH), all novel strains were placed in a phylogenetic group with five species of the genus Bradyrhizobium that nodulate soybeans. The combination of phenotypic characteristics from several tests including carbon and nitrogen source utilization and antibiotic resistance could be used to differentiate representative strains from recognized species of the genus Bradyrhizobium. Novel strain OO99(T) elicits effective nodules on Glycine max, Glycine soja and Macroptilium atropurpureum, partially effective nodules on Desmodium canadense and Vigna unguiculata, and ineffective nodules on Amphicarpaea bracteata and Phaseolus vulgaris. Based on the data presented, we conclude that our strains represent a novel species for which the name Bradyrhizobium ottawaense sp. nov. is proposed, with OO99(T) ( = LMG 26739(T) = HAMBI 3284(T)) as the type strain. The DNA G+C content is 62.6 mol%.

Citing Articles

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Bromfield E, Cloutier S Antonie Van Leeuwenhoek. 2024; 117(1):69.

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Bradyrhizobium ottawaense efficiently reduces nitrous oxide through high nosZ gene expression.

Wasai-Hara S, Itakura M, Fernandes Siqueira A, Takemoto D, Sugawara M, Mitsui H Sci Rep. 2023; 13(1):18862.

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sp. nov. strain T173 isolated from (sweet clover) in Canada possesses recombinant plasmid pT173b harbouring symbiosis and type IV secretion system genes apparently acquired from .

Bromfield E, Cloutier S, Hynes M Front Microbiol. 2023; 14:1195755.

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Unique Rhizobial Communities Dominated by Bradyrhizobium liaoningense and Bradyrhizobium ottawaense were Found in Vegetable Soybean Nodules in Osaka Prefecture, Japan.

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