In Vivo Quantification of Mouse Autoimmune Arthritis by PET/CT

Overview

Journal Int J Rheum Dis

Specialty Rheumatology

Date 2014 Jun 27

PMID 24965561

Citations 4

Authors

Smriti Kundu-Raychaudhuri

Anupam Mitra

Ananya Datta-Mitra

Abhijit J Chaudhari

Siba P Raychaudhuri

Affiliations

Soon will be listed here.

Abstract

Aim: To quantify the progression and severity of mouse collagen-induced arthritis (CIA) using an in vivo imaging tool, (18) F-fluorodeoxyglucose ((18) F-FDG) PET/CT and validate it against gold standard 'histopathological' evaluation.

Method: The PET radiotracer (18) F-FDG, a marker for glucose metabolism, was injected in mice at different stages of CIA and the radiotracer distribution was imaged using a PET scanner. A sequential CT scan provided correlated anatomy. Radiotracer concentration was derived from PET/CT images for individual limb joints and on a per-limb basis at different stages of the disease. The imaging outcomes were subjected to correlation analysis with concurrently measured clinical and histological score.

Results: Clinical and histological score, and hence disease severity, showed a strong linear correlation (r(2) = 0.71, P = 0.001 and r(2) = 0.87, P < 0.001, respectively) with radiotracer concentration measured from PET/CT during the progression of CIA.

Conclusions: The strong positive correlation of the (18) F-FDG PET/CT findings with the histopathological evaluation at different stages of the disease suggest the potential of this imaging tool for the non-invasive assessment of progression and severity in mouse autoimmune arthritis. Thus, in preclinical studies, (18) F-FDG PET/CT can be considered as a non-invasive tool to develop novel therapies of inflammatory arthritis.

Citing Articles

Development of an Ultra High Resolution PET Scanner for Imaging Rodent Paws: PawPET.

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Quantitative tracking of inflammatory activity at the peak and trough plasma levels of tofacitinib, a Janus kinase inhibitor, via in vivo F-FDG PET.

Raychaudhuri S, Abria C, Harmany Z, Smith C, Kundu-Raychaudhuri S, Raychaudhuri S Int J Rheum Dis. 2019; 22(12):2165-2169.

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A model of chronic enthesitis and new bone formation characterized by multimodal imaging.

Czegley C, Gillmann C, Schauer C, Seyler L, Reinwald C, Hahn M Dis Model Mech. 2018; 11(9).

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In-vivo quantitative assessment of the therapeutic response in a mouse model of collagen-induced arthritis using F-fluorodeoxyglucose positron emission tomography.

Mitra A, Kundu-Raychaudhuri S, Abria C, Rona A, Chaudhari A, Raychaudhuri S Clin Exp Immunol. 2017; 188(2):293-298.

PMID: 28090641 PMC: 5383438. DOI: 10.1111/cei.12926.

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