Developmental Changes in Cell Proliferation and Apoptosis in the Normal Duck Bursa of Fabricius

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2014 Jun 26

PMID 24962417

Citations 2

Authors

Jing Fang

Xi Peng

Affiliations

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Abstract

The aim of this work was to investigate developmental changes in cell proliferation and apoptosis in normal duck bursa of Fabricius using flow cytometry and immunohistochemistry. Studies were carried out on Tianfu ducks on days 24 and 27 of embryogenesis (E24 and E27) along with days 20, 70, and 200 of postnatal development (P20, P70, and P200). Results showed that the percentage of G0/G1 bursa cells significantly increased between E24 and P200 while the percentage of cells in the S phase or G2 + M phase as well as the proliferating index obviously decreased during the same period. Proliferation cell nuclear antigen was detected in lymphocyte and interfollicular epithelium. The proliferative lymphocyte density tended to decrease from E24 to P200. Apoptotic bodies in macrophages, free apoptotic bodies, or nuclei with condensed chromatin in lymphocytes in follicles were identified by transferase-mediated dUTP nick-end labeling. Both flow cytometry and microscopic analysis reveal that the proportion of apoptotic cells and apoptotic lymphocyte density increased from E24 to P20, fell on P70, then rose again on P200. Our foundings demonstrate that cell proliferation decreases and apoptosis increases with age. These changes may account for duck bursa development and involution.

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References

Peng X, Cui Y, Cui W, Deng J, Cui H, Yang F . The cell cycle arrest and apoptosis of bursa of Fabricius induced by low selenium in chickens. Biol Trace Elem Res. 2010; 139(1):32-40. DOI: 10.1007/s12011-010-8639-8. View

Harrison L, Brown C, Afonso C, Zhang J, Susta L . Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of Newcastle disease virus of varying virulence. J Comp Pathol. 2011; 145(4):327-35. DOI: 10.1016/j.jcpa.2011.03.005. View

Rodriguez-Mendez A, Luna-Acosta J, Carranza M, Harvey S, Aramburo C, Luna M . Growth hormone expression in stromal and non-stromal cells in the bursa of Fabricius during bursal development and involution: Causal relationships?. Gen Comp Endocrinol. 2010; 167(2):297-307. DOI: 10.1016/j.ygcen.2010.03.019. View

Higgins S, Berghman L, Moore R, Caldwell D, Caldwell D, Tizard I . In situ detection and quantification of bursa of fabricius cellular proliferation or apoptosis in normal or steroid-treated neonatal chicks. Poult Sci. 2002; 81(8):1136-41. DOI: 10.1093/ps/81.8.1136. View

Sayegh C, Ratcliffe M . Perinatal deletion of B cells expressing surface Ig molecules that lack V(D)J-encoded determinants in the bursa of Fabricius is not due to intrafollicular competition. J Immunol. 2000; 164(10):5041-8. DOI: 10.4049/jimmunol.164.10.5041. View

Paramithiotis E, Ratcliffe M . B cell emigration directly from the cortex of lymphoid follicles in the bursa of Fabricius. Eur J Immunol. 1994; 24(2):458-63. DOI: 10.1002/eji.1830240229. View

Lydyard P, Grossi C, Cooper M . Ontogeny of B cells in the chicken. I. Sequential development of clonal diversity in the bursa. J Exp Med. 1976; 144(1):79-97. PMC: 2190360. DOI: 10.1084/jem.144.1.79. View

Kang Z, Bedecarrats G, Zadworny D . Expression patterns of the prolactin receptor gene in chicken lymphoid tissues during embryogenesis and posthatch period. Poult Sci. 2007; 86(11):2404-12. DOI: 10.3382/ps.2007-00235. View

Motyka B, Reynolds J . Apoptosis is associated with the extensive B cell death in the sheep ileal Peyer's patch and the chicken bursa of Fabricius: a possible role in B cell selection. Eur J Immunol. 1991; 21(8):1951-8. DOI: 10.1002/eji.1830210825. View

10.

Neiman P, Thomas S, Loring G . Induction of apoptosis during normal and neoplastic B-cell development in the bursa of Fabricius. Proc Natl Acad Sci U S A. 1991; 88(13):5857-61. PMC: 51977. DOI: 10.1073/pnas.88.13.5857. View

11.

Franchini A, Ottaviani E . Immunoreactive POMC-derived peptides and cytokines in the chicken thymus and bursa of Fabricius microenvironments: age-related changes. J Neuroendocrinol. 1999; 11(9):685-92. DOI: 10.1046/j.1365-2826.1999.00385.x. View

12.

Paramithiotis E, Ratcliffe M . Bursa-dependent subpopulations of peripheral B lymphocytes in chicken blood. Eur J Immunol. 1993; 23(1):96-102. DOI: 10.1002/eji.1830230116. View

13.

Saifuddin M, Manktelow B, Moriarty K, Christensen N, Birtles M . Age-related functional changes in the follicle-associated epithelium of the bursa of Fabricius in Shaver cockerels. N Z Vet J. 1988; 36(3):108-11. DOI: 10.1080/00480169.1988.35502. View

14.

Guiping Y, Anchun C, Mingshu W, Xiaoying H, Yi Z, Fei L . Preliminary study on duck enteritis virus-induced lymphocyte apoptosis in vivo. Avian Dis. 2007; 51(2):546-9. DOI: 10.1637/0005-2086(2007)51[546:PSODEV]2.0.CO;2. View

15.

Olah I, Glick B . Follicle-associated epithelium and medullary epithelial tissue of the bursa of fabricius are two different compartments. Anat Rec. 1992; 233(4):577-87. DOI: 10.1002/ar.1092330412. View

16.

Weill J, REYNAUD C . The chicken B cell compartment. Science. 1987; 238(4830):1094-8. DOI: 10.1126/science.3317827. View

17.

Ciriaco E, Pinera P, Diaz-Esnal B, Laura R . Age-related changes in the avian primary lymphoid organs (thymus and bursa of Fabricius). Microsc Res Tech. 2003; 62(6):482-7. DOI: 10.1002/jemt.10416. View

18.

Melnikova V, Afanaseva M, Sapozhnikov A, Zakharova L . [Dynamics of apoptosis and proliferation in the rat thymus and spleen during perinatal development]. Ontogenez. 2006; 37(4):286-91. View

19.

Glick B . Embryogenesis of the bursa of Fabricius: stem cell, microenvironment, and receptor-paracrine pathways. Poult Sci. 1995; 74(3):419-26. DOI: 10.3382/ps.0740419. View

20.

Glick B . Historical perspective: the bursa of Fabricius and its influence on B-cell development, past and present. Vet Immunol Immunopathol. 1991; 30(1):3-12. DOI: 10.1016/0165-2427(91)90003-u. View