MiR-212 Promotes Pancreatic Cancer Cell Growth and Invasion by Targeting the Hedgehog Signaling Pathway Receptor Patched-1

Overview

Journal J Exp Clin Cancer Res

Publisher Biomed Central

Specialty Oncology

Date 2014 Jun 26

PMID 24961235

Citations 39

Authors

Chenchao Ma

Kate Nong

Bo Wu

Bo Dong

Yueqing Bai

Hongda Zhu

Weiwei Wang

Xinyu Huang

Zhou Yuan

Kaixing Ai

Affiliations

Soon will be listed here.

Abstract

Background: microRNAs (miRNAs) are a class of small non-coding RNAs that play important roles in carcinogenesis. In the present study, we investigated the effect of miR-212 on pancreatic ductal adenocarcinoma (PDAC) and its target protein.

Methods: Quantitative real-time PCR(qRT-PCR) was performed to detect the expression of miR-212 in PDAC tissues and pancreatic cancer cell lines. miR-212 mimic, miR-212 inhibitor and negative control were transfected into pancreatic cancer cells and the effect of miR-212 up-regulation and down-regulation on the proliferation, migration and invasion of cells were investigated. Furthermore, the mRNA and protein levels of Patched-1(PTCH1) were measured. Meanwhile, luciferase assays were performed to validate PTCH1 as miR-212 target in PDAC.

Results: miR-212 was up-regulated in PDAC tissues and cells.Using both gain-of function and loss-of function experiments, a pro-oncogenic function of miR-212 was demonstrated in PDAC. Moreover, up-regulated of PTCH1 could attenuate the effect induced by miR-212.

Conclusion: These data suggest that miR-212 could facilitate PDAC progression and metastasis through targeting PTCH1, implicating a novel mechanism for the progression of PDAC.

Citing Articles

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Functional and Potential Therapeutic Implication of MicroRNAs in Pancreatic Cancer.

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