Convenient, Sensitive and High-throughput Method for Screening Botanic Origin

Overview

Journal Sci Rep

Specialty Science

Date 2014 Jun 24

PMID 24953704

Citations 4

Authors

Yuan Yuan

Chao Jiang

Libing Liu

Shulin Yu

Zhanhu Cui

Min Chen

Shufang Lin

Shu Wang

Luqi Huang

Affiliations

Soon will be listed here.

Abstract

In this work, a rapid (within 4-5 h), sensitive and visible new method for assessing botanic origin is developed by combining loop-mediated isothermal amplification with cationic conjugated polymers. The two Chinese medicinal materials (Jin-Yin-Hua and Shan-Yin-Hua) with similar morphology and chemical composition were clearly distinguished by gene SNP genotyping assays. The identification of plant species in Patented Chinese drugs containing Lonicera buds is successfully performed using this detection system. The method is also robust enough to be used in high-throughput screening. This new method is very helpful to identify herbal materials, and is beneficial for detecting safety and quality of botanic products.

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