RNA Interference of 1-aminocyclopropane-1-carboxylic Acid Oxidase (ACO1 and ACO2) Genes Expression Prolongs the Shelf Life of Eksotika (Carica Papaya L.) Papaya Fruit

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2014 Jun 21

PMID 24950439

Citations 5

Authors

Rogayah Sekeli

Janna Ong Abdullah

Parameswari Namasivayam

Pauziah Muda

Umi Kalsom Abu Bakar

Wee Chien Yeong

Vilasini Pillai

Affiliations

Soon will be listed here.

Abstract

The purpose of this study was to evaluate the effectiveness of using RNA interference in down regulating the expression of 1-aminocyclopropane-1-carboxylic acid oxidase gene in Eksotika papaya. One-month old embryogenic calli were separately transformed with Agrobacterium strain LBA 4404 harbouring the three different RNAi pOpOff2 constructs bearing the 1-aminocyclopropane-1-carboxylic acid oxidase gene. A total of 176 putative transformed lines were produced from 15,000 calli transformed, selected, then regenerated on medium supplemented with kanamycin. Integration and expression of the targeted gene in putatively transformed lines were verified by PCR and real-time RT-PCR. Confined field evaluation of a total of 31 putative transgenic lines planted showed a knockdown expression of the targeted ACO1 and ACO2 genes in 13 lines, which required more than 8 days to achieve the full yellow colour (Index 6). Fruits harvested from lines pRNAiACO2 L2-9 and pRNAiACO1 L2 exhibited about 20 and 14 days extended post-harvest shelf life to reach Index 6, respectively. The total soluble solids contents of the fruits ranged from 11 to 14° Brix, a range similar to fruits from non-transformed, wild type seed-derived plants.

Citing Articles

Targeted modification of by CRISPR/Cas9 extends the shelf-life of var. melon.

Nonaka S, Ito M, Ezura H Front Genome Ed. 2023; 5:1176125.

PMID: 37304010 PMC: 10249633. DOI: 10.3389/fgeed.2023.1176125.

DNA methylation is involved in the regulation of pepper fruit ripening and interacts with phytohormones.

Xiao K, Chen J, He Q, Wang Y, Shen H, Sun L J Exp Bot. 2020; 71(6):1928-1942.

PMID: 31907544 PMC: 7242076. DOI: 10.1093/jxb/eraa003.

Effect of astragalosides on long non-coding RNA expression profiles in rats with adjuvant-induced arthritis.

Jiang H, Wu F, Liu J, Qin X, Jiang N, Li W Int J Mol Med. 2019; 44(4):1344-1356.

PMID: 31364738 PMC: 6713426. DOI: 10.3892/ijmm.2019.4281.

1-Aminocyclopropane-1-Carboxylic Acid Oxidase (ACO): The Enzyme That Makes the Plant Hormone Ethylene.

Houben M, Van de Poel B Front Plant Sci. 2019; 10:695.

PMID: 31191592 PMC: 6549523. DOI: 10.3389/fpls.2019.00695.

CtACO1 Overexpression Resulted in the Alteration of the Flavonoids Profile of Safflower.

Tu Y, He B, Gao S, Guo D, Jia X, Dong X Molecules. 2019; 24(6).

PMID: 30901924 PMC: 6471848. DOI: 10.3390/molecules24061128.

References

Day C, Lee E, Kobayashi J, Holappa L, Albert H, Ow D . Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced. Genes Dev. 2000; 14(22):2869-80. PMC: 317066. DOI: 10.1101/gad.849600. View

Matzke A, Matzke M . Position effects and epigenetic silencing of plant transgenes. Curr Opin Plant Biol. 1999; 1(2):142-8. DOI: 10.1016/s1369-5266(98)80016-2. View

Kooter , Matzke , MEYER . Listening to the silent genes: transgene silencing, gene regulation and pathogen control. Trends Plant Sci. 1999; 4(9):340-347. DOI: 10.1016/s1360-1385(99)01467-3. View

Rauschhuber C, Mueck-Haeusl M, Zhang W, Nettelbeck D, Ehrhardt A . RNAi suppressor P19 can be broadly exploited for enhanced adenovirus replication and microRNA knockdown experiments. Sci Rep. 2013; 3:1363. PMC: 3586813. DOI: 10.1038/srep01363. View

Barton G, Medzhitov R . Retroviral delivery of small interfering RNA into primary cells. Proc Natl Acad Sci U S A. 2002; 99(23):14943-5. PMC: 137524. DOI: 10.1073/pnas.242594499. View

Li J, Jiang D, Zhou H, Li F, Yang J, Hong L . Expression of RNA-interference/antisense transgenes by the cognate promoters of target genes is a better gene-silencing strategy to study gene functions in rice. PLoS One. 2011; 6(3):e17444. PMC: 3048295. DOI: 10.1371/journal.pone.0017444. View

Samalova M, Brzobohaty B, Moore I . pOp6/LhGR: a stringently regulated and highly responsive dexamethasone-inducible gene expression system for tobacco. Plant J. 2005; 41(6):919-35. DOI: 10.1111/j.1365-313X.2005.02341.x. View

Napoli C, Lemieux C, Jorgensen R . Introduction of a Chimeric Chalcone Synthase Gene into Petunia Results in Reversible Co-Suppression of Homologous Genes in trans. Plant Cell. 1990; 2(4):279-289. PMC: 159885. DOI: 10.1105/tpc.2.4.279. View

Craft J, Samalova M, Baroux C, Townley H, Martinez A, Jepson I . New pOp/LhG4 vectors for stringent glucocorticoid-dependent transgene expression in Arabidopsis. Plant J. 2005; 41(6):899-918. DOI: 10.1111/j.1365-313X.2005.02342.x. View

10.

Mazumder B, Seshadri V, Fox P . Translational control by the 3'-UTR: the ends specify the means. Trends Biochem Sci. 2003; 28(2):91-8. DOI: 10.1016/S0968-0004(03)00002-1. View

11.

Ouwerkerk P, De Kam R, Hoge J, Meijer A . Glucocorticoid-inducible gene expression in rice. Planta. 2001; 213(3):370-8. DOI: 10.1007/s004250100583. View

12.

Miki D, Itoh R, Shimamoto K . RNA silencing of single and multiple members in a gene family of rice. Plant Physiol. 2005; 138(4):1903-13. PMC: 1183382. DOI: 10.1104/pp.105.063933. View

13.

Kuderova A, Urbankova I, Valkova M, Malbeck J, Brzobohaty B, Nemethova D . Effects of conditional IPT-dependent cytokinin overproduction on root architecture of Arabidopsis seedlings. Plant Cell Physiol. 2008; 49(4):570-82. DOI: 10.1093/pcp/pcn029. View

14.

Schijlen E, de Vos C, Martens S, Jonker H, Rosin F, Molthoff J . RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits. Plant Physiol. 2007; 144(3):1520-30. PMC: 1914118. DOI: 10.1104/pp.107.100305. View

15.

Andersen S, Cvitanich C, Hougaard B, Roussis A, Gronlund M, Jensen D . The glucocorticoid-inducible GVG system causes severe growth defects in both root and shoot of the model legume Lotus japonicus. Mol Plant Microbe Interact. 2003; 16(12):1069-76. DOI: 10.1094/MPMI.2003.16.12.1069. View

16.

Rothermel A, Volpert K, Burghardt M, Lantzsch C, Robitzki A, Layer P . Knock-down of GFRalpha4 expression by RNA interference affects the development of retinal cell types in three-dimensional histiotypic retinal spheres. Invest Ophthalmol Vis Sci. 2006; 47(6):2716-25. DOI: 10.1167/iovs.05-1472. View

17.

Wang D, Kennedy S, Conte Jr D, Kim J, Gabel H, Kamath R . Somatic misexpression of germline P granules and enhanced RNA interference in retinoblastoma pathway mutants. Nature. 2005; 436(7050):593-7. DOI: 10.1038/nature04010. View

18.

Kevany B, Taylor M, Klee H . Fruit-specific suppression of the ethylene receptor LeETR4 results in early-ripening tomato fruit. Plant Biotechnol J. 2007; 6(3):295-300. DOI: 10.1111/j.1467-7652.2007.00319.x. View

19.

Zhai Z, Sooksa-Nguan T, Vatamaniuk O . Establishing RNA interference as a reverse-genetic approach for gene functional analysis in protoplasts. Plant Physiol. 2008; 149(2):642-52. PMC: 2633838. DOI: 10.1104/pp.108.130260. View