Biofilm Formation by Staphylococcus Aureus from Food Contact Surfaces in a Meat-based Broth and Sensitivity to Sanitizers

Overview

Journal Braz J Microbiol

Specialty Microbiology

Date 2014 Jun 21

PMID 24948915

Citations 26

Authors

Evandro Leite de Souza

Quenia Gramile Silva Meira

Isabella de Medeiros Barbosa

Ana Julia Alves Aguiar Athayde

Maria Lucia da Conceicao

Jose Pinto De Siqueira Junior

Affiliations

Soon will be listed here.

Abstract

This study assessed the capacity of adhesion, the detachment kinetic and the biofilm formation by Staphylococcus aureus isolated from food services on stainless steel and polypropylene surfaces (2 × 2 cm) when cultivated in a meat-based broth at 28 and 7 °C. It was also to study the efficacy of the sanitizers sodium hypochlorite (250 mg/L) and peracetic acid (30 mg/L) in inactivating the bacterial cells in the preformed biofilm. S. aureus strains adhered in high numbers regardless the assayed surface kind and incubation temperature over 72 h. Cells detachment of surfaces revealed high persistence over the incubation period. Number of cells needed for biofilm formation was noted at all experimental systems already after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered on polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacity to adhere and form biofilm on polypropylene and stainless steel surfaces under different growth conditions. Moreover, the cells in biofilm matrix were resistant for total removal when submitted to the exposure to sanitizers.

Citing Articles

Assessment of prevalence, antibiotic resistance, and virulence profiles of biofilm-forming isolated from raw seafood in Bangladesh.

Ullah M, Islam M, Ferdous F, Rana M, Hassan J, Rahman M Heliyon. 2024; 10(20):e39294.

PMID: 39640770 PMC: 11620263. DOI: 10.1016/j.heliyon.2024.e39294.

A scalable route to quaternary ammonium-functionalized AgCl colloidal antimicrobials inhibiting food pathogenic bacteria and biofilms.

Bajrami D, Hossain S, Barbarossa A, Sportelli M, Picca R, Gentile L Heliyon. 2024; 10(3):e25260.

PMID: 38327442 PMC: 10847915. DOI: 10.1016/j.heliyon.2024.e25260.

The behaviour of and in bottled mineral water.

Schalli M, Platzer S, Haas D, Reinthaler F Heliyon. 2023; 9(11):e21634.

PMID: 38027778 PMC: 10643266. DOI: 10.1016/j.heliyon.2023.e21634.

Resistance Profiles and Virulence Determinants in Biofilm-Forming Isolated from Raw Seafood in Bangladesh.

Ullah M, Islam M, Rana M, Ferdous F, Neloy F, Firdous Z Pathogens. 2023; 12(9).

PMID: 37764909 PMC: 10535238. DOI: 10.3390/pathogens12091101.

Biofilm-Producing Ability of Obtained from Surfaces and Milk of Mastitic Cows.

Vargova M, Zigo F, Vyrostkova J, Farkasova Z, Rehan I Vet Sci. 2023; 10(6).

PMID: 37368772 PMC: 10303978. DOI: 10.3390/vetsci10060386.

References

Kumar C, Anand S . Significance of microbial biofilms in food industry: a review. Int J Food Microbiol. 1998; 42(1-2):9-27. DOI: 10.1016/s0168-1605(98)00060-9. View

Fux C, Wilson S, Stoodley P . Detachment characteristics and oxacillin resistance of Staphyloccocus aureus biofilm emboli in an in vitro catheter infection model. J Bacteriol. 2004; 186(14):4486-91. PMC: 438612. DOI: 10.1128/JB.186.14.4486-4491.2004. View

Barnes L, Lo M, Adams M, Chamberlain A . Effect of milk proteins on adhesion of bacteria to stainless steel surfaces. Appl Environ Microbiol. 1999; 65(10):4543-8. PMC: 91605. DOI: 10.1128/AEM.65.10.4543-4548.1999. View

Zoltai P, Zottola E, McKay L . Scanning Electron Microscopy of Microbial Attachment to Milk Contact Surfaces . J Food Prot. 2019; 44(3):204-208. DOI: 10.4315/0362-028X-44.3.204. View

van Houdt R, Michiels C . Biofilm formation and the food industry, a focus on the bacterial outer surface. J Appl Microbiol. 2010; 109(4):1117-31. DOI: 10.1111/j.1365-2672.2010.04756.x. View

Cheng G, Zhang Z, Chen S, Bryers J, Jiang S . Inhibition of bacterial adhesion and biofilm formation on zwitterionic surfaces. Biomaterials. 2007; 28(29):4192-9. PMC: 5463736. DOI: 10.1016/j.biomaterials.2007.05.041. View

Rossoni E, Gaylarde C . Comparison of sodium hypochlorite and peracetic acid as sanitising agents for stainless steel food processing surfaces using epifluorescence microscopy. Int J Food Microbiol. 2000; 61(1):81-5. DOI: 10.1016/s0168-1605(00)00369-x. View

Zottola E, Sasahara K . Microbial biofilms in the food processing industry--should they be a concern?. Int J Food Microbiol. 1994; 23(2):125-48. DOI: 10.1016/0168-1605(94)90047-7. View

Spoering A, Lewis K . Biofilms and planktonic cells of Pseudomonas aeruginosa have similar resistance to killing by antimicrobials. J Bacteriol. 2001; 183(23):6746-51. PMC: 95513. DOI: 10.1128/JB.183.23.6746-6751.2001. View

10.

Herrera J, Cabo M, Gonzalez A, Pazos I, Pastoriza L . Adhesion and detachment kinetics of several strains of Staphylococcus aureus subsp. aureus under three different experimental conditions. Food Microbiol. 2007; 24(6):585-91. DOI: 10.1016/j.fm.2007.01.001. View

11.

Costerton J, Stewart P, Greenberg E . Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284(5418):1318-22. DOI: 10.1126/science.284.5418.1318. View

12.

Carson C, Mee B, Riley T . Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy. Antimicrob Agents Chemother. 2002; 46(6):1914-20. PMC: 127210. DOI: 10.1128/AAC.46.6.1914-1920.2002. View

13.

Braga L, Shupp J, Cummings C, Jett M, Takahashi J, Carmo L . Pomegranate extract inhibits Staphylococcus aureus growth and subsequent enterotoxin production. J Ethnopharmacol. 2004; 96(1-2):335-9. DOI: 10.1016/j.jep.2004.08.034. View

14.

Ronner A, Wong A . Biofilm Development and Sanitizer Inactivation of Listeria monocytogenes and Salmonella typhimurium on Stainless Steel and Buna-n Rubber. J Food Prot. 2019; 56(9):750-758. DOI: 10.4315/0362-028X-56.9.750. View

15.

Kuzma L, Rozalski M, Walencka E, Rozalska B, Wysokinska H . Antimicrobial activity of diterpenoids from hairy roots of Salvia sclarea L.: salvipisone as a potential anti-biofilm agent active against antibiotic resistant Staphylococci. Phytomedicine. 2006; 14(1):31-5. DOI: 10.1016/j.phymed.2005.10.008. View

16.

Heydorn A, Nielsen A, Hentzer M, Sternberg C, Givskov M, Ersboll B . Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology (Reading). 2000; 146 ( Pt 10):2395-2407. DOI: 10.1099/00221287-146-10-2395. View

17.

Bruhn J, Haagensen J, Bagge-Ravn D, Gram L . Culture conditions of Roseobacter strain 27-4 affect its attachment and biofilm formation as quantified by real-time PCR. Appl Environ Microbiol. 2006; 72(4):3011-5. PMC: 1449026. DOI: 10.1128/AEM.72.4.3011-3015.2006. View

18.

Rode T, Langsrud S, Holck A, Moretro T . Different patterns of biofilm formation in Staphylococcus aureus under food-related stress conditions. Int J Food Microbiol. 2007; 116(3):372-83. DOI: 10.1016/j.ijfoodmicro.2007.02.017. View

19.

Moretro T, Hermansen L, Holck A, Sidhu M, Rudi K, Langsrud S . Biofilm formation and the presence of the intercellular adhesion locus ica among staphylococci from food and food processing environments. Appl Environ Microbiol. 2003; 69(9):5648-55. PMC: 194930. DOI: 10.1128/AEM.69.9.5648-5655.2003. View

20.

Revol-Junelles A, Miguindou-Mabiala R, Roger-Maigne D, Milliere J . Behavior of Escherichia coli cells and Bacillus cereus spores on poplar wood crates by impedance measurements. J Food Prot. 2005; 68(1):80-4. DOI: 10.4315/0362-028x-68.1.80. View