Ultrastructural Study of Electron Dense Deposits in Renal Tubular Basement Membrane: Prevalence and Relationship to Epithelial Atrophy

Overview

Journal Ultrastruct Pathol

Publisher Informa Healthcare

Date 2014 Jun 17

PMID 24933115

Citations 2

Authors

Jim L C Yong

Murray C Killingsworth

Affiliations

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Abstract

This study reports the prevalence of immune deposits associated with the proximal and distal tubules in a series of routine renal biopsies received in our department during a single calendar year. From 87 cases, 65 (74%) were found to have glomerular immune deposits by immunofluorescence. Tubular immune deposits were found in 12 cases (18%), 3 of which had no glomerular deposits. By transmission electron microscopy (EM), 58 cases (66%) were found to have deposits of granular or vesicular material associated with the tubular basement membranes (TBM). Finely granular electron dense deposits appeared to correspond to the immune deposits seen by immunofluorescence microscopy (IF) and may be a sensitive marker of immune deposition.

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References

Tomioka M, Ueki K, Nakahashi H, Isoda A, Kuroiwa T, Kaneko Y . Widespread crystalline inclusions affecting podocytes, tubular cells and interstitial histiocytes in the myeloma kidney. Clin Nephrol. 2004; 62(3):229-33. DOI: 10.5414/cnp62229. View

Christensen E, Birn H, Storm T, Weyer K, Nielsen R . Endocytic receptors in the renal proximal tubule. Physiology (Bethesda). 2012; 27(4):223-36. DOI: 10.1152/physiol.00022.2012. View

Hemminger J, Satoskar A, Brodsky S, Calomeni E, Nadasdy G, Kovach P . Unique pattern of renal κ light chain amyloid deposition with histiocytic transdifferentiation of tubular epithelial cells. Am J Surg Pathol. 2012; 36(8):1253-7. DOI: 10.1097/PAS.0b013e31825b845e. View

Sinniah R, Khan T . Renal tubular basement membrane changes in tubulointerstitial damage in patients with glomerular diseases. Ultrastruct Pathol. 2000; 23(6):359-68. DOI: 10.1080/019131299281329. View

Kapur U, Barton K, Fresco R, Leehey D, Leehy D, Picken M . Expanding the pathologic spectrum of immunoglobulin light chain proximal tubulopathy. Arch Pathol Lab Med. 2007; 131(9):1368-72. DOI: 10.5858/2007-131-1368-ETPSOI. View

Tojo A, Kinugasa S . Mechanisms of glomerular albumin filtration and tubular reabsorption. Int J Nephrol. 2012; 2012:481520. PMC: 3363986. DOI: 10.1155/2012/481520. View

Yong J, Killingsworth M . Acute light chain tubulopathy in myeloma. Pathology. 2009; 41(3):300-2. DOI: 10.1080/00313020902756352. View

Denker B, Sabath E . The biology of epithelial cell tight junctions in the kidney. J Am Soc Nephrol. 2011; 22(4):622-5. DOI: 10.1681/ASN.2010090922. View

Fan J, Ng Y, Hill P, Nikolic-Paterson D, Mu W, Atkins R . Transforming growth factor-beta regulates tubular epithelial-myofibroblast transdifferentiation in vitro. Kidney Int. 1999; 56(4):1455-67. DOI: 10.1046/j.1523-1755.1999.00656.x. View

10.

Fujigaki Y, Kimura M, Asano M, Suzuki T, Hishida A . Ultrastructure of tubular epithelial cells in response to microembolism-induced chronic ischemic injury in rats. Nephron Exp Nephrol. 2003; 95(4):e144-51. DOI: 10.1159/000074841. View

11.

Yong J, Warren B . A practical approach to the diagnosis of renal disease by biopsy. Pathology. 1994; 26(4):370-96. DOI: 10.1080/00313029400169032. View

12.

Theilig F, Kriz W, Jerichow T, Schrade P, Hahnel B, Willnow T . Abrogation of protein uptake through megalin-deficient proximal tubules does not safeguard against tubulointerstitial injury. J Am Soc Nephrol. 2007; 18(6):1824-34. DOI: 10.1681/ASN.2006111266. View

13.

Kumar D, Robertson S, Burns K . Evidence of apoptosis in human diabetic kidney. Mol Cell Biochem. 2004; 259(1-2):67-70. DOI: 10.1023/b:mcbi.0000021346.03260.7e. View

14.

Pallet N, Bouvier N, Legendre C, Gilleron J, Codogno P, Beaune P . Autophagy protects renal tubular cells against cyclosporine toxicity. Autophagy. 2008; 4(6):783-91. DOI: 10.4161/auto.6477. View

15.

Ibrini J, Fadel S, Chana R, Brunskill N, Wagner B, Johnson T . Albumin-induced epithelial mesenchymal transformation. Nephron Exp Nephrol. 2012; 120(3):e91-102. DOI: 10.1159/000336822. View

16.

Collan Y, Lahdevirta J, Jokinen E . Electron microscopy of nephropathia epidemica. Renal tubular basement membrane. Am J Pathol. 1978; 92(1):167-72. PMC: 2018600. View

17.

Faulkner J, Szcykalski L, Springer F, Barnes J . Origin of interstitial fibroblasts in an accelerated model of angiotensin II-induced renal fibrosis. Am J Pathol. 2005; 167(5):1193-205. PMC: 1603794. DOI: 10.1016/S0002-9440(10)61208-4. View