Phospho-proteomic Analysis of Developmental Reprogramming in the Moss Physcomitrella Patens

Biological Significance: Protoplast regeneration is an ideal model system for investigating developmental reprogramming in plants. Here, for Physcomitrella patens, we characterize the phospho-proteome for protonemata, protoplasts made therefrom, and for protonemata regenerated from the protoplasts for 2d. Among the 519 proteins with functional annotation in fresh protoplasts and protoplasts regenerated for 2d, proteins involved in epigenetic modification, post-transcriptional gene regulation, hormone signal transduction, and meristem maintenance have been reported to be important for expression of developmental reprogramming. Together, our study presents a more complete understanding of the plant cell's developmental reprogramming.