Ultrasensitive Immuno-detection Using Viral Nanoparticles with Modular Assembly Using Genetically-directed Biotinylation

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2014 Jun 16

PMID 24930095

Citations 6

Authors

Julia Litvinov

Anna E V Hagstrom

Yubitza Lopez

Meenu Adhikari

Katerina Kourentzi

Ulrich Strych

Federico A Monzon

William Foster

Philip T Cagle

Richard C Willson

Affiliations

Soon will be listed here.

Abstract

We report a novel, modular approach to immuno-detection based on antibody recognition and PCR read-out that employs antibody-conjugated bacteriophage and easily-manipulated non-pathogenic viruses as affinity agents. Our platform employs phage genetically tagged for in vivo biotinylation during phage maturation that can easily be linked, through avidin, to any biotinylated affinity agent, including full-length antibodies, peptides, lectins or aptamers. The presence of analyte is reported with high sensitivity through real-time PCR. This approach avoids the need to clone antibody-encoding DNA fragments, allows the use of full-length, high affinity antibodies and, by having DNA reporters naturally encapsulated inside the bacteriophage, greatly reduces nonspecific binding of DNA. We validate the efficacy of this new approach through the detection of Vascular Endothelial Growth Factor, a known angiogenic cancer biomarker protein, at attomolar concentrations in bronchoalveolar lavage fluid.

Citing Articles

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Aptamer-Phage Reporters for Ultrasensitive Lateral Flow Assays.

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