Isolation of a CDNA Encoding Mitochondrial Citrate Synthase from Arabidopsis Thaliana

Overview

Journal Plant Mol Biol

Date 1989 Oct 1

PMID 2491664

Citations 11

Authors

E A Unger

J M Hand

A R Cashmore

A C Vasconcelos

Affiliations

Soon will be listed here.

Abstract

We isolated a cDNA clone from Arabidopsis thaliana encoding the TCA cycle enzyme, citrate synthase. The plant enzyme displays 48% and 44% amino acid residue similarity with the pig, and yeast polypeptides, respectively. Many proteins, including citrate synthase, which are destined to reside in organelles such as mitochondria and chloroplasts, are the products of the nucleocytoplasmic protein synthesizing machinery and are imported post-translationally to the site of function. We present preliminary investigations toward the establishment of an in vitro plant mitochondrial import system allowing for future studies to dissect this process in plants where the cell must differentiate between mitochondria and chloroplast and direct their polypeptides appropriately.

Citing Articles

Aluminum relieves fluoride stress through stimulation of organic acid production in .

Pan J, Li D, Zhu J, Shu Z, Ye X, Xing A Physiol Mol Biol Plants. 2020; 26(6):1127-1137.

PMID: 32549678 PMC: 7266864. DOI: 10.1007/s12298-020-00813-2.

Mitochondrial biogenesis and function in Arabidopsis.

Millar A, Small I, Day D, Whelan J Arabidopsis Book. 2012; 6:e0111.

PMID: 22303236 PMC: 3243404. DOI: 10.1199/tab.0111.

One novel mitochondrial citrate synthase from Oryza sativa L. can enhance aluminum tolerance in transgenic tobacco.

Han Y, Zhang W, Zhang B, Zhang S, Wang W, Ming F Mol Biotechnol. 2009; 42(3):299-305.

PMID: 19326262 DOI: 10.1007/s12033-009-9162-z.

Mitochondrial malate dehydrogenase from corn : purification of multiple forms.

Hayes M, Luethy M, Elthon T Plant Physiol. 1991; 97(4):1381-7.

PMID: 16668560 PMC: 1081175. DOI: 10.1104/pp.97.4.1381.

Higher plant mitochondria .

Mackenzie , McIntosh Plant Cell. 1999; 11(4):571-86.

PMID: 10213779 PMC: 144202. DOI: 10.1105/tpc.11.4.571.

References

Bloxham D, Parmelee D, Kumar S, Walsh K, Titani K . Complete amino acid sequence of porcine heart citrate synthase. Biochemistry. 1982; 21(9):2028-36. DOI: 10.1021/bi00538a009. View

Duckworth H, Bell A . Large-scale production of citrate synthase from a cloned gene. Can J Biochem. 1982; 60(12):1143-7. DOI: 10.1139/o82-146. View

Guest J . Hybrid plasmids containing the citrate synthase gene (gltA) of Escherichia coli K12. J Gen Microbiol. 1981; 124(1):17-23. DOI: 10.1099/00221287-124-1-17. View

Meyerowitz E . Arabidopsis thaliana. Annu Rev Genet. 1987; 21:93-111. DOI: 10.1146/annurev.ge.21.120187.000521. View

Murray M, Thompson W . Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980; 8(19):4321-5. PMC: 324241. DOI: 10.1093/nar/8.19.4321. View

Feinberg A, Vogelstein B . "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984; 137(1):266-7. DOI: 10.1016/0003-2697(84)90381-6. View

Pullen A, Budgen N, Danson M, Hough D . Citrate synthase: an immunochemical investigation of interspecies diversity. FEBS Lett. 1985; 182(1):163-6. DOI: 10.1016/0014-5793(85)81175-3. View

Schatz G, Butow R . How are proteins imported into mitochondria?. Cell. 1983; 32(2):316-8. DOI: 10.1016/0092-8674(83)90450-6. View

Remington S, Wiegand G, Huber R . Crystallographic refinement and atomic models of two different forms of citrate synthase at 2.7 and 1.7 A resolution. J Mol Biol. 1982; 158(1):111-52. DOI: 10.1016/0022-2836(82)90452-1. View

10.

Chen W, DOUGLAS M . Phosphodiester bond cleavage outside mitochondria is required for the completion of protein import into the mitochondrial matrix. Cell. 1987; 49(5):651-8. DOI: 10.1016/0092-8674(87)90541-1. View

11.

Laskey R . The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis. Methods Enzymol. 1980; 65(1):363-71. DOI: 10.1016/s0076-6879(80)65047-2. View

12.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

13.

Boutry M, Nagy F, Poulsen C, Aoyagi K, Chua N . Targeting of bacterial chloramphenicol acetyltransferase to mitochondria in transgenic plants. Nature. 1987; 328(6128):340-2. DOI: 10.1038/328340a0. View

14.

Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M . Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984; 12(18):7035-56. PMC: 320141. DOI: 10.1093/nar/12.18.7035. View

15.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

16.

White J, Scandalios J . In vitro synthesis, importation and processing of Mn-superoxide dismutase (SOD-3) into maize mitochondria. Biochim Biophys Acta. 1987; 926(1):16-25. DOI: 10.1016/0304-4165(87)90178-4. View

17.

Kozak M . Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984; 12(2):857-72. PMC: 318541. DOI: 10.1093/nar/12.2.857. View

18.

Henikoff S . Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984; 28(3):351-9. DOI: 10.1016/0378-1119(84)90153-7. View

19.

Unger E, Vasconcelos A . Purification and characterization of mitochondrial citrate synthase. Plant Physiol. 1989; 89(3):719-23. PMC: 1055911. DOI: 10.1104/pp.89.3.719. View

20.

Benton W, Davis R . Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977; 196(4286):180-2. DOI: 10.1126/science.322279. View