Homeostatic Control of Polo-like Kinase-1 Engenders Non-genetic Heterogeneity in G2 Checkpoint Fidelity and Timing

Overview

Journal Nat Commun

Specialty Biology

Date 2014 Jun 5

PMID 24893992

Citations 20

Authors

Hongqing Liang

Alessandro Esposito

Siddharth De

Suzan Ber

Philippe Collin

Uttam Surana

Ashok R Venkitaraman

Affiliations

Soon will be listed here.

Abstract

The G2 checkpoint monitors DNA damage, preventing mitotic entry until the damage can be resolved. The mechanisms controlling checkpoint recovery are unclear. Here, we identify non-genetic heterogeneity in the fidelity and timing of damage-induced G2 checkpoint enforcement in individual cells from the same population. Single-cell fluorescence imaging reveals that individual damaged cells experience varying durations of G2 arrest, and recover with varying levels of remaining checkpoint signal or DNA damage. A gating mechanism dependent on polo-like kinase-1 (PLK1) activity underlies this heterogeneity. PLK1 activity continually accumulates from initial levels in G2-arrested cells, at a rate inversely correlated to checkpoint activation, until it reaches a threshold allowing mitotic entry regardless of remaining checkpoint signal or DNA damage. Thus, homeostatic control of PLK1 by the dynamic opposition between checkpoint signalling and pro-mitotic activities heterogeneously enforces the G2 checkpoint in each individual cell, with implications for cancer pathogenesis and therapy.

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