Alpha-mangostin Suppresses IL-6 and IL-8 Expression in P. Gingivalis LPS-stimulated Human Gingival Fibroblasts

Overview

Journal Odontology

Specialty Dentistry

Date 2014 Jun 4

PMID 24888491

Citations 14

Authors

Ichaya Yiemwattana

Ruchadaporn Kaomongkolgit

Affiliations

Soon will be listed here.

Abstract

This study aims to investigate the effect of alpha-mangostin on interleukin (IL)-6 and IL-8 expression in human gingival fibroblasts (HGFs). HGFs were challenged with Porphyromonas gingivalis LPS and then treated with various concentrations of alpha-mangostin. The cytotoxicity was determined using MTS assay and cytokine expressions were evaluated by Real-time PCR and ELISA. The results showed that 5 μg/ml P. gingivalis LPS and alpha-mangostin at 1 µg/ml or less did not affect the viability of HGFs. Alpha-mangostin reduced IL-6 and IL-8 mRNA and protein in P. gingivalis LPS-stimulated HGFs. These findings suggested that alpha-mangostin might be used as an adjunct to the periodontal therapy.

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References

Kaomongkolgit R . Alpha-mangostin suppresses MMP-2 and MMP-9 expression in head and neck squamous carcinoma cells. Odontology. 2012; 101(2):227-32. DOI: 10.1007/s10266-012-0081-2. View

Holt S, Kesavalu L, Walker S, Genco C . Virulence factors of Porphyromonas gingivalis. Periodontol 2000. 1999; 20:168-238. DOI: 10.1111/j.1600-0757.1999.tb00162.x. View

DAiuto F, Parkar M, Andreou G, Suvan J, Brett P, Ready D . Periodontitis and systemic inflammation: control of the local infection is associated with a reduction in serum inflammatory markers. J Dent Res. 2004; 83(2):156-60. DOI: 10.1177/154405910408300214. View

Abate W, Alghaithy A, Parton J, Jones K, Jackson S . Surfactant lipids regulate LPS-induced interleukin-8 production in A549 lung epithelial cells by inhibiting translocation of TLR4 into lipid raft domains. J Lipid Res. 2009; 51(2):334-44. PMC: 2803235. DOI: 10.1194/jlr.M000513. View

Feng Z, Weinberg A . Role of bacteria in health and disease of periodontal tissues. Periodontol 2000. 2006; 40:50-76. DOI: 10.1111/j.1600-0757.2005.00148.x. View

Imatani T, Kato T, Okuda K . Production of inflammatory cytokines by human gingival fibroblasts stimulated by cell-surface preparations of Porphyromonas gingivalis. Oral Microbiol Immunol. 2001; 16(2):65-72. DOI: 10.1034/j.1399-302x.2001.016002065.x. View

Chung R, Grbic J, Lamster I . Interleukin-8 and beta-glucuronidase in gingival crevicular fluid. J Clin Periodontol. 1997; 24(3):146-52. DOI: 10.1111/j.1600-051x.1997.tb00483.x. View

Bumrungpert A, Kalpravidh R, Chuang C, Overman A, Martinez K, Kennedy A . Xanthones from mangosteen inhibit inflammation in human macrophages and in human adipocytes exposed to macrophage-conditioned media. J Nutr. 2010; 140(4):842-7. DOI: 10.3945/jn.109.120022. View

Goutoudi P, Diza E, Arvanitidou M . Effect of periodontal therapy on crevicular fluid interleukin-6 and interleukin-8 levels in chronic periodontitis. Int J Dent. 2011; 2012:362905. PMC: 3205686. DOI: 10.1155/2012/362905. View

10.

Gutierrez-Orozco F, Chitchumroonchokchai C, Lesinski G, Suksamrarn S, Failla M . α-Mangostin: anti-inflammatory activity and metabolism by human cells. J Agric Food Chem. 2013; 61(16):3891-900. PMC: 3793015. DOI: 10.1021/jf4004434. View

11.

Okada N, Kobayashi M, Mugikura K, Okamatsu Y, Hanazawa S, Kitano S . Interleukin-6 production in human fibroblasts derived from periodontal tissues is differentially regulated by cytokines and a glucocorticoid. J Periodontal Res. 1997; 32(7):559-69. DOI: 10.1111/j.1600-0765.1997.tb00932.x. View

12.

Liu S, Lee L, Hu N, Huange K, Shih Y, Munekazu I . Effects of alpha-mangostin on the expression of anti-inflammatory genes in U937 cells. Chin Med. 2012; 7(1):19. PMC: 3476971. DOI: 10.1186/1749-8546-7-19. View

13.

Ikewaki N, Inoko H . A very late activating antigen-alpha4 (CD49d) monoclonal antibody, BU49 induces phosphorylation of a cAMP response element-binding protein (CREB), resulting in induction of homotypic cell aggregation and enhancement of interleukin-8 (IL-8) production. Microbiol Immunol. 2002; 46(10):685-95. DOI: 10.1111/j.1348-0421.2002.tb02752.x. View

14.

Ishihara Y, Nishihara T, Kuroyanagi T, Shirozu N, Yamagishi E, Ohguchi M . Gingival crevicular interleukin-1 and interleukin-1 receptor antagonist levels in periodontally healthy and diseased sites. J Periodontal Res. 1997; 32(6):524-9. DOI: 10.1111/j.1600-0765.1997.tb00568.x. View

15.

Gutierrez-Orozco F, Failla M . Biological activities and bioavailability of mangosteen xanthones: a critical review of the current evidence. Nutrients. 2013; 5(8):3163-83. PMC: 3775248. DOI: 10.3390/nu5083163. View

16.

Bumrungpert A, Kalpravidh R, Chitchumroonchokchai C, Chuang C, West T, Kennedy A . Xanthones from mangosteen prevent lipopolysaccharide-mediated inflammation and insulin resistance in primary cultures of human adipocytes. J Nutr. 2009; 139(6):1185-91. DOI: 10.3945/jn.109.106617. View

17.

Wozniak A, Betts W, Murphy G, Rokicinski M . Interleukin-8 primes human neutrophils for enhanced superoxide anion production. Immunology. 1993; 79(4):608-15. PMC: 1421931. View