Characterization of Metastasis Formation and Virotherapy in the Human C33A Cervical Cancer Model

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 Jun 3

PMID 24887184

Citations 8

Authors

Ulrike Donat

Juliane Rother

Simon Schafer

Michael Hess

Barbara Hartl

Christina Kober

Johanna Langbein-Laugwitz

Jochen Stritzker

Nanhai G Chen

Richard J Aguilar

Stephanie Weibel

Aladar A Szalay

Affiliations

Soon will be listed here.

Abstract

More than 90% of cancer mortalities are due to cancer that has metastasized. Therefore, it is crucial to intensify research on metastasis formation and therapy. Here, we describe for the first time the metastasizing ability of the human cervical cancer cell line C33A in athymic nude mice after subcutaneous implantation of tumor cells. In this model, we demonstrated a steady progression of lumbar and renal lymph node metastases during tumor development. Besides predominantly occurring lymphatic metastases, we visualized the formation of hematogenous metastases utilizing red fluorescent protein (RFP) expressing C33A-RFP cells. RFP positive cancer cells were found migrating in blood vessels and forming micrometastases in lungs of tumor-bearing mice. Next, we set out to analyze the influence of oncolytic virotherapy in the C33A-RFP model and demonstrated an efficient virus-mediated reduction of tumor size and metastatic burden. These results suggest the C33A-RFP cervical cancer model as a new platform to analyze cancer metastases as well as to test novel treatment options to combat metastases.

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