Overexpression of Arabidopsis MYB96 Confers Drought Resistance in Camelina Sativa Via Cuticular Wax Accumulation

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2014 Jun 2

PMID 24880908

Citations 52

Authors

Saet Buyl Lee

Hyojin Kim

Ryeo Jin Kim

Mi Chung Suh

Affiliations

Soon will be listed here.

Abstract

Camelina has been highlighted as an emerging oilseed crop. Transgenic Camelina plants overexpressing Arabidopsis MYB96 exhibited drought resistance by activating expression of Camelina wax biosynthetic genes and accumulating wax load. Camelina (Camelina sativa L.) is an oilseed crop in the Brassicaeae family with potential to expand biofuel production to marginal land. The aerial portion of all land plants is covered with cuticular wax to protect them from desiccation. In this study, the Arabidopsis MYB96 gene was overexpressed in Camelina under the control of the CaMV35S promoter. Transgenic Camelina plants overexpressing Arabidopsis MYB96 exhibited normal growth and development and enhanced tolerance to drought. Deposition of epicuticular wax crystals and total wax loads increased significantly on the surfaces of transgenic leaves compared with that of non-transgenic plants. The levels of alkanes and primary alcohols prominently increased in transgenic Camelina plants relative to non-transgenic plants. Cuticular transpiration occurred more slowly in transgenic leaves than that in non-transgenic plants. Genome-wide identification of Camelina wax biosynthetic genes enabled us to determine that the expression levels of CsKCS2, CsKCS6, CsKCR1-1, CsKCR1-2, CsECR, and CsMAH1 were approximately two to sevenfold higher in transgenic Camelina leaves than those in non-transgenic leaves. These results indicate that MYB96-mediated transcriptional regulation of wax biosynthetic genes is an approach applicable to generating drought-resistant transgenic crops. Transgenic Camelina plants with enhanced drought tolerance could be cultivated on marginal land to produce renewable biofuels and biomaterials.

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References

Bourdenx B, Bernard A, Domergue F, Pascal S, Leger A, Roby D . Overexpression of Arabidopsis ECERIFERUM1 promotes wax very-long-chain alkane biosynthesis and influences plant response to biotic and abiotic stresses. Plant Physiol. 2011; 156(1):29-45. PMC: 3091054. DOI: 10.1104/pp.111.172320. View

Oshima Y, Shikata M, Koyama T, Ohtsubo N, Mitsuda N, Ohme-Takagi M . MIXTA-like transcription factors and WAX INDUCER1/SHINE1 coordinately regulate cuticle development in Arabidopsis and Torenia fournieri. Plant Cell. 2013; 25(5):1609-24. PMC: 3694695. DOI: 10.1105/tpc.113.110783. View

Kim K, Park S, Jenks M . Changes in leaf cuticular waxes of sesame (Sesamum indicum L.) plants exposed to water deficit. J Plant Physiol. 2006; 164(9):1134-43. DOI: 10.1016/j.jplph.2006.07.004. View

Jung K, Han M, Lee D, Lee Y, Schreiber L, Franke R . Wax-deficient anther1 is involved in cuticle and wax production in rice anther walls and is required for pollen development. Plant Cell. 2006; 18(11):3015-32. PMC: 1693940. DOI: 10.1105/tpc.106.042044. View

Bernard A, Joubes J . Arabidopsis cuticular waxes: advances in synthesis, export and regulation. Prog Lipid Res. 2012; 52(1):110-29. DOI: 10.1016/j.plipres.2012.10.002. View

Jetter R, Kunst L . Plant surface lipid biosynthetic pathways and their utility for metabolic engineering of waxes and hydrocarbon biofuels. Plant J. 2008; 54(4):670-83. DOI: 10.1111/j.1365-313X.2008.03467.x. View

Li Y, Beisson F, Koo A, Molina I, Pollard M, Ohlrogge J . Identification of acyltransferases required for cutin biosynthesis and production of cutin with suberin-like monomers. Proc Natl Acad Sci U S A. 2007; 104(46):18339-44. PMC: 2084344. DOI: 10.1073/pnas.0706984104. View

Lee S, Suh M . Recent advances in cuticular wax biosynthesis and its regulation in Arabidopsis. Mol Plant. 2012; 6(2):246-9. DOI: 10.1093/mp/sss159. View

Yang M, Yang Q, Fu T, Zhou Y . Overexpression of the Brassica napus BnLAS gene in Arabidopsis affects plant development and increases drought tolerance. Plant Cell Rep. 2010; 30(3):373-88. DOI: 10.1007/s00299-010-0940-7. View

10.

Li F, Wu X, Lam P, Bird D, Zheng H, Samuels L . Identification of the wax ester synthase/acyl-coenzyme A: diacylglycerol acyltransferase WSD1 required for stem wax ester biosynthesis in Arabidopsis. Plant Physiol. 2008; 148(1):97-107. PMC: 2528131. DOI: 10.1104/pp.108.123471. View

11.

Dyer J, Stymne S, Green A, Carlsson A . High-value oils from plants. Plant J. 2008; 54(4):640-55. DOI: 10.1111/j.1365-313X.2008.03430.x. View

12.

Haslam T, Kunst L . Extending the story of very-long-chain fatty acid elongation. Plant Sci. 2013; 210:93-107. DOI: 10.1016/j.plantsci.2013.05.008. View

13.

Wang Y, Wan L, Zhang L, Zhang Z, Zhang H, Quan R . An ethylene response factor OsWR1 responsive to drought stress transcriptionally activates wax synthesis related genes and increases wax production in rice. Plant Mol Biol. 2011; 78(3):275-88. DOI: 10.1007/s11103-011-9861-2. View

14.

Hutcheon C, Ditt R, Beilstein M, Comai L, Schroeder J, Goldstein E . Polyploid genome of Camelina sativa revealed by isolation of fatty acid synthesis genes. BMC Plant Biol. 2010; 10:233. PMC: 3017853. DOI: 10.1186/1471-2229-10-233. View

15.

Seo P, Xiang F, Qiao M, Park J, Lee Y, Kim S . The MYB96 transcription factor mediates abscisic acid signaling during drought stress response in Arabidopsis. Plant Physiol. 2009; 151(1):275-89. PMC: 2735973. DOI: 10.1104/pp.109.144220. View

16.

Stracke R, WERBER M, Weisshaar B . The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol. 2001; 4(5):447-56. DOI: 10.1016/s1369-5266(00)00199-0. View

17.

Chen X, Truksa M, Snyder C, El-Mezawy A, Shah S, Weselake R . Three homologous genes encoding sn-glycerol-3-phosphate acyltransferase 4 exhibit different expression patterns and functional divergence in Brassica napus. Plant Physiol. 2010; 155(2):851-65. PMC: 3032471. DOI: 10.1104/pp.110.169482. View

18.

Yang J, Ordiz M, Jaworski J, Beachy R . Induced accumulation of cuticular waxes enhances drought tolerance in Arabidopsis by changes in development of stomata. Plant Physiol Biochem. 2011; 49(12):1448-55. DOI: 10.1016/j.plaphy.2011.09.006. View

19.

Nelson D, Repetti P, Adams T, Creelman R, Wu J, Warner D . Plant nuclear factor Y (NF-Y) B subunits confer drought tolerance and lead to improved corn yields on water-limited acres. Proc Natl Acad Sci U S A. 2007; 104(42):16450-5. PMC: 2034233. DOI: 10.1073/pnas.0707193104. View

20.

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View