A Protective Role of Methionine-R-sulfoxide Reductase Against Cadmium in Schizosaccharomyces Pombe

Overview

Journal J Microbiol

Specialty Microbiology

Date 2014 Jun 1

PMID 24879348

Citations 1

Authors

Chang-Jin Lim

Hannah Jo

Kyunghoon Kim

Affiliations

Soon will be listed here.

Abstract

The Schizosaccharomyces pombe cells harboring the methionine- R-sulfoxide reductase (MsrB)-overexpressing recombinant plasmid pFMetSO exhibited better growth than vector control cells, when shifted into fresh medium containing cadmium chloride (abbreviated as Cd). Although both groups of cells contained enhanced reactive oxygen species (ROS) and nitric oxide (NO) levels in the presence of Cd, ROS and NO levels were significantly lower in the S. pombe cells harboring pFMetSO than in vector control cells. Conversely, the S. pombe cells harboring pFMetSO possessed higher total glutathione (GSH) levels and a greater reduced/oxidized GSH ratio than vector control cells under the same conditions.

Citing Articles

Strain: Arsenic(III)/Cadmium(II) Accumulation, Tolerance, and Simulation Application in Environmental Remediation.

Zhang Y, Chen X, Xie L Int J Environ Res Public Health. 2023; 20(6).

PMID: 36981967 PMC: 10049176. DOI: 10.3390/ijerph20065056.

References

Navarrete C, Siles A, Martinez J, Calero F, Ramos J . Oxidative stress sensitivity in Debaryomyces hansenii. FEMS Yeast Res. 2009; 9(4):582-90. DOI: 10.1111/j.1567-1364.2009.00500.x. View

Le D, Lee B, Marino S, Zhang Y, Fomenko D, Kaya A . Functional analysis of free methionine-R-sulfoxide reductase from Saccharomyces cerevisiae. J Biol Chem. 2008; 284(7):4354-64. PMC: 2640979. DOI: 10.1074/jbc.M805891200. View

Li Z, Lu Y, Zhen R, Szczypka M, Thiele D, Rea P . A new pathway for vacuolar cadmium sequestration in Saccharomyces cerevisiae: YCF1-catalyzed transport of bis(glutathionato)cadmium. Proc Natl Acad Sci U S A. 1997; 94(1):42-7. PMC: 19233. DOI: 10.1073/pnas.94.1.42. View

Ortiz D, Ruscitti T, McCue K, Ow D . Transport of metal-binding peptides by HMT1, a fission yeast ABC-type vacuolar membrane protein. J Biol Chem. 1995; 270(9):4721-8. DOI: 10.1074/jbc.270.9.4721. View

Kiani-Esfahani A, Tavalaee M, Deemeh M, Hamiditabar M, Nasr-Esfahani M . DHR123: an alternative probe for assessment of ROS in human spermatozoa. Syst Biol Reprod Med. 2012; 58(3):168-74. DOI: 10.3109/19396368.2012.681420. View

Sherman M, Aeberhard E, Wong V, Griscavage J, Ignarro L . Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem Biophys Res Commun. 1993; 191(3):1301-8. DOI: 10.1006/bbrc.1993.1359. View

Mielniczki-Pereira A, Hahn A, Bonatto D, Riger C, Eleutherio E, Henriques J . New insights into the Ca2+-ATPases that contribute to cadmium tolerance in yeast. Toxicol Lett. 2011; 207(2):104-11. DOI: 10.1016/j.toxlet.2011.08.023. View

Baudouin-Cornu P, LABARRE J . Regulation of the cadmium stress response through SCF-like ubiquitin ligases: comparison between Saccharomyces cerevisiae, Schizosaccharomyces pombe and mammalian cells. Biochimie. 2006; 88(11):1673-85. DOI: 10.1016/j.biochi.2006.03.001. View

Groppa M, Rosales E, Iannone M, Benavides M . Nitric oxide, polyamines and Cd-induced phytotoxicity in wheat roots. Phytochemistry. 2008; 69(14):2609-15. DOI: 10.1016/j.phytochem.2008.07.016. View

10.

Gardarin A, Chedin S, Lagniel G, Aude J, Godat E, Catty P . Endoplasmic reticulum is a major target of cadmium toxicity in yeast. Mol Microbiol. 2010; 76(4):1034-48. DOI: 10.1111/j.1365-2958.2010.07166.x. View

11.

Vido K, Spector D, Lagniel G, Lopez S, Toledano M, LABARRE J . A proteome analysis of the cadmium response in Saccharomyces cerevisiae. J Biol Chem. 2000; 276(11):8469-74. DOI: 10.1074/jbc.M008708200. View

12.

Clemens S, Kim E, Neumann D, Schroeder J . Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. EMBO J. 1999; 18(12):3325-33. PMC: 1171413. DOI: 10.1093/emboj/18.12.3325. View

13.

Zhao C, Hartke A, La Sorda M, Posteraro B, Laplace J, Auffray Y . Role of methionine sulfoxide reductases A and B of Enterococcus faecalis in oxidative stress and virulence. Infect Immun. 2010; 78(9):3889-97. PMC: 2937430. DOI: 10.1128/IAI.00165-10. View

14.

Lopez E, Arce C, Oset-Gasque M, Canadas S, Gonzalez M . Cadmium induces reactive oxygen species generation and lipid peroxidation in cortical neurons in culture. Free Radic Biol Med. 2006; 40(6):940-51. DOI: 10.1016/j.freeradbiomed.2005.10.062. View

15.

Silver S, Phung L . Bacterial heavy metal resistance: new surprises. Annu Rev Microbiol. 1996; 50:753-89. DOI: 10.1146/annurev.micro.50.1.753. View

16.

Hwang G, Sasaki K, Takahashi T, Yamamoto R, Naganuma A . Overexpression of Ycg1 or Ydr520c confers resistance to cadmium in Saccharomyces cerevisiae. J Toxicol Sci. 2009; 34(4):441-3. DOI: 10.2131/jts.34.441. View

17.

Cuypers A, Plusquin M, Remans T, Jozefczak M, Keunen E, Gielen H . Cadmium stress: an oxidative challenge. Biometals. 2010; 23(5):927-40. DOI: 10.1007/s10534-010-9329-x. View

18.

Wink D, Miranda K, Espey M . Cytotoxicity related to oxidative and nitrosative stress by nitric oxide. Exp Biol Med (Maywood). 2001; 226(7):621-3. DOI: 10.1177/153537020222600704. View

19.

Filipic M . Mechanisms of cadmium induced genomic instability. Mutat Res. 2011; 733(1-2):69-77. DOI: 10.1016/j.mrfmmm.2011.09.002. View

20.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View