Intravital Imaging of Mouse Colonic Adenoma Using MMP-based Molecular Probes with Multi-channel Fluorescence Endoscopy

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2014 May 31

PMID 24877024

Citations 14

Authors

Gyungseok Oh

Su Woong Yoo

Yebin Jung

Yeon-Mi Ryu

Youngrong Park

Sang-Yeob Kim

Ki Hean Kim

Sungjee Kim

Seung-Jae Myung

Euiheon Chung

Affiliations

Soon will be listed here.

Abstract

Intravital imaging has provided molecular, cellular and anatomical insight into the study of tumor. Early detection and treatment of gastrointestinal (GI) diseases can be enhanced with specific molecular markers and endoscopic imaging modalities. We present a wide-field multi-channel fluorescence endoscope to screen GI tract for colon cancer using multiple molecular probes targeting matrix metalloproteinases (MMP) conjugated with quantum dots (QD) in AOM/DSS mouse model. MMP9 and MMP14 antibody (Ab)-QD conjugates demonstrate specific binding to colonic adenoma. The average target-to-background (T/B) ratios are 2.10 ± 0.28 and 1.78 ± 0.18 for MMP14 Ab-QD and MMP9 Ab-QD, respectively. The overlap between the two molecular probes is 67.7 ± 8.4%. The presence of false negative indicates that even more number of targeting could increase the sensitivity of overall detection given heterogeneous molecular expression in tumors. Our approach indicates potential for the screening of small or flat lesions that are precancerous.

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