Crystal Structure of Penicillin-binding Protein 3 (PBP3) from Escherichia Coli

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 May 31

PMID 24875494

Citations 25

Authors

Eric Sauvage

Adeline Derouaux

Claudine Fraipont

Marine Joris

Raphael Herman

Mathieu Rocaboy

Marie Schloesser

Jacques Dumas

Frederic Kerff

Martine Nguyen-Disteche

Paulette Charlier

Affiliations

Soon will be listed here.

Abstract

In Escherichia coli, penicillin-binding protein 3 (PBP3), also known as FtsI, is a central component of the divisome, catalyzing cross-linking of the cell wall peptidoglycan during cell division. PBP3 is mainly periplasmic, with a 23 residues cytoplasmic tail and a single transmembrane helix. We have solved the crystal structure of a soluble form of PBP3 (PBP3(57-577)) at 2.5 Å revealing the two modules of high molecular weight class B PBPs, a carboxy terminal module exhibiting transpeptidase activity and an amino terminal module of unknown function. To gain additional insight, the PBP3 Val88-Ser165 subdomain (PBP3(88-165)), for which the electron density is poorly defined in the PBP3 crystal, was produced and its structure solved by SAD phasing at 2.1 Å. The structure shows a three dimensional domain swapping with a β-strand of one molecule inserted between two strands of the paired molecule, suggesting a possible role in PBP3(57-577) dimerization.

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