Role of Alpha-synuclein in Autophagy Modulation of Primary Human T Lymphocytes

Overview

Journal Cell Death Dis

Specialties Cell Biology
General Medicine

Date 2014 May 31

PMID 24874737

Citations 27

Authors

T Colasanti

M Vomero

C Alessandri

C Barbati

A Maselli

C Camperio

F Conti

A Tinari

C Carlo-Stella

L Tuosto

D Benincasa

G Valesini

W Malorni

M Pierdominici

E Ortona

Affiliations

Soon will be listed here.

Abstract

It has been demonstrated that α-synuclein can aggregate and contribute to the pathogenesis of some neurodegenerative diseases and it is capable of hindering autophagy in neuronal cells. Here, we investigated the implication of α-synuclein in the autophagy process in primary human T lymphocytes. We provide evidence that: (i) knocking down of the α-synuclein gene resulted in increased autophagy, (ii) autophagy induction by energy deprivation was associated with a significant decrease of α-synuclein levels, (iii) autophagy inhibition by 3-methyladenine or by ATG5 knocking down led to a significant increase of α-synuclein levels, and (iv) autophagy impairment, constitutive in T lymphocytes from patients with systemic lupus erythematosus, was associated with abnormal accumulation of α-synuclein aggregates. These results suggest that α-synuclein could be considered as an autophagy-related marker of peripheral blood lymphocytes, potentially suitable for use in the clinical practice.

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