Identification of Glutathione S-transferase Genes Responding to Pathogen Infestation in Populus Tomentosa

Overview

Journal Funct Integr Genomics

Publisher Springer

Specialties Genetics
Molecular Biology

Date 2014 May 30

PMID 24870810

Citations 23

Authors

Weihua Liao

Lexiang Ji

Jia Wang

Zhong Chen

Meixia Ye

Huandi Ma

Xinmin An

Affiliations

Soon will be listed here.

Abstract

Stem blister canker, caused by Botryosphaeria dothidea, is becoming the most serious disease of poplar in China. The molecular basis of the poplar in response to stem blister canker is not well understood. To reveal the global transcriptional changes of poplar to infection by B. dothidea, Solexa paired-end sequencing of complementary DNAs (cDNAs) from control (NB) and pathogen-treated samples (WB) was performed, resulting in a total of 339,283 transcripts and 183,881 unigenes. A total of 206,586 transcripts were differentially expressed in response to pathogen stress (false discovery rate ≤0.05 and an absolute value of log2Ratio (NB/WB) ≥1). In enrichment analysis, energy metabolism and redox reaction-related macromolecules were accumulated significantly in Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses, indicating components of dynamic defense against the fungus. A total of 852 transcripts (575 upregulated and 277 downregulated transcripts) potentially involved in plant-pathogen interaction were also differentially regulated, including genes encoding proteins linked to signal transduction (putative leucine-rich repeat (LRR) protein kinases and calcium-binding proteins), defense (pathogenesis-related protein 1), and cofactors (jasmonate-ZIM-domain-containing proteins and heat shock proteins). Moreover, transcripts encoding glutathione S-transferase (GST) were accumulated to high levels, revealing key genes and proteins potentially related to pathogen resistance. Poplar RNA sequence data were validated by quantitative real-time PCR (RT-qPCR), which revealed a highly reliability of the transcriptomic profiling data.

Citing Articles

A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression.

Xie Q, Ahmed U, Qi C, Du K, Luo J, Wang P For Res (Fayettev). 2024; 4:e021.

PMID: 39524407 PMC: 11524287. DOI: 10.48130/forres-0024-0017.

Editing Metabolism, Sex, and Microbiome: How Can We Help Poplar Resist Pathogens?.

Kovalev M, Gladysh N, Bogdanova A, Bolsheva N, Popchenko M, Kudryavtseva A Int J Mol Sci. 2024; 25(2).

PMID: 38279306 PMC: 10816636. DOI: 10.3390/ijms25021308.

and Act as Critical Links between Growth and Immunity in Cotton.

Peng S, Li P, Li T, Tian Z, Xu R Int J Mol Sci. 2024; 25(1).

PMID: 38203172 PMC: 10778622. DOI: 10.3390/ijms25010001.

Transcriptomics Profiling of Molecular Mechanism against Freezing Stress.

Han Z, Xu X, Zhang S, Zhao Q, Li H, Cui Y Int J Mol Sci. 2022; 23(23).

PMID: 36499002 PMC: 9737005. DOI: 10.3390/ijms232314676.

Overexpression of salicylic acid methyltransferase reduces salicylic acid-mediated pathogen resistance in poplar.

Dong H, Zhang W, Li Y, Feng Y, Wang X, Liu Z Front Plant Sci. 2022; 13:973305.

PMID: 36388494 PMC: 9660245. DOI: 10.3389/fpls.2022.973305.

References

Grabherr M, Haas B, Yassour M, Levin J, Thompson D, Amit I . Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011; 29(7):644-52. PMC: 3571712. DOI: 10.1038/nbt.1883. View

Chain F, Cote-Beaulieu C, Belzile F, Menzies J, Belanger R . A comprehensive transcriptomic analysis of the effect of silicon on wheat plants under control and pathogen stress conditions. Mol Plant Microbe Interact. 2009; 22(11):1323-30. DOI: 10.1094/MPMI-22-11-1323. View

Martin F, Gianinazzi-Pearson V, Hijri M, Lammers P, Requena N, Sanders I . The long hard road to a completed Glomus intraradices genome. New Phytol. 2009; 180(4):747-50. DOI: 10.1111/j.1469-8137.2008.02671.x. View

Santamaria M, Thomson C, Read N, Loake G . The promoter of a basic PR1-like gene, AtPRB1, from Arabidopsis establishes an organ-specific expression pattern and responsiveness to ethylene and methyl jasmonate. Plant Mol Biol. 2001; 47(5):641-52. DOI: 10.1023/a:1012410009930. View

Shimaoka T, Ohnishi M, Sazuka T, Mitsuhashi N, Hara-Nishimura I, Shimazaki K . Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana. Plant Cell Physiol. 2004; 45(6):672-83. DOI: 10.1093/pcp/pch099. View

DITTRICH H, Kutchan T . Molecular cloning, expression, and induction of berberine bridge enzyme, an enzyme essential to the formation of benzophenanthridine alkaloids in the response of plants to pathogenic attack. Proc Natl Acad Sci U S A. 1991; 88(22):9969-73. PMC: 52848. DOI: 10.1073/pnas.88.22.9969. View

Asai T, Tena G, Plotnikova J, Willmann M, Chiu W, Gomez-Gomez L . MAP kinase signalling cascade in Arabidopsis innate immunity. Nature. 2002; 415(6875):977-83. DOI: 10.1038/415977a. View

Passos M, de Cruz V, Emediato F, de Teixeira C, Azevedo V, Brasileiro A . Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola: gene assembly, annotation and marker development. BMC Genomics. 2013; 14:78. PMC: 3635893. DOI: 10.1186/1471-2164-14-78. View

Martinelli F, Reagan R, Uratsu S, Phu M, Albrecht U, Zhao W . Gene regulatory networks elucidating huanglongbing disease mechanisms. PLoS One. 2013; 8(9):e74256. PMC: 3783430. DOI: 10.1371/journal.pone.0074256. View

10.

Dang Z, Zheng L, Wang J, Gao Z, Wu S, Qi Z . Transcriptomic profiling of the salt-stress response in the wild recretohalophyte Reaumuria trigyna. BMC Genomics. 2013; 14:29. PMC: 3562145. DOI: 10.1186/1471-2164-14-29. View

11.

Dixon D, Hawkins T, Hussey P, Edwards R . Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily. J Exp Bot. 2009; 60(4):1207-18. PMC: 2657551. DOI: 10.1093/jxb/ern365. View

12.

Adhikari B, Savory E, Vaillancourt B, Childs K, Hamilton J, Day B . Expression profiling of Cucumis sativus in response to infection by Pseudoperonospora cubensis. PLoS One. 2012; 7(4):e34954. PMC: 3335828. DOI: 10.1371/journal.pone.0034954. View

13.

Giraud E, Ivanova A, Gordon C, Whelan J, Considine M . Sulphur dioxide evokes a large scale reprogramming of the grape berry transcriptome associated with oxidative signalling and biotic defence responses. Plant Cell Environ. 2011; 35(2):405-17. DOI: 10.1111/j.1365-3040.2011.02379.x. View

14.

Poovaiah B, Du L, Wang H, Yang T . Recent advances in calcium/calmodulin-mediated signaling with an emphasis on plant-microbe interactions. Plant Physiol. 2013; 163(2):531-42. PMC: 3793035. DOI: 10.1104/pp.113.220780. View

15.

Niderman T, Genetet I, Bruyere T, Gees R, Stintzi A, Legrand M . Pathogenesis-related PR-1 proteins are antifungal. Isolation and characterization of three 14-kilodalton proteins of tomato and of a basic PR-1 of tobacco with inhibitory activity against Phytophthora infestans. Plant Physiol. 1995; 108(1):17-27. PMC: 157301. DOI: 10.1104/pp.108.1.17. View

16.

Babashpour S, Aminzadeh S, Farrokhi N, Karkhane A, Haghbeen K . Characterization of a chitinase (Chit62) from Serratia marcescens B4A and its efficacy as a bioshield against plant fungal pathogens. Biochem Genet. 2012; 50(9-10):722-35. DOI: 10.1007/s10528-012-9515-3. View

17.

Kaku H, Nishizawa Y, Ishii-Minami N, Akimoto-Tomiyama C, Dohmae N, Takio K . Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor. Proc Natl Acad Sci U S A. 2006; 103(29):11086-91. PMC: 1636686. DOI: 10.1073/pnas.0508882103. View

18.

Salem M, Rexroad 3rd C, Wang J, Thorgaard G, Yao J . Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches. BMC Genomics. 2010; 11:564. PMC: 3091713. DOI: 10.1186/1471-2164-11-564. View

19.

Gotz S, Garcia-Gomez J, Terol J, Williams T, Nagaraj S, Nueda M . High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Res. 2008; 36(10):3420-35. PMC: 2425479. DOI: 10.1093/nar/gkn176. View

20.

Varshney R, Nayak S, May G, Jackson S . Next-generation sequencing technologies and their implications for crop genetics and breeding. Trends Biotechnol. 2009; 27(9):522-30. DOI: 10.1016/j.tibtech.2009.05.006. View