Synchronized Dual Pulse Gastric Electrical Stimulation Induces Activation of Enteric Glial Cells in Rats with Diabetic Gastroparesis

Overview

Journal Gastroenterol Res Pract

Publisher Wiley

Specialty Gastroenterology

Date 2014 May 27

PMID 24860604

Citations 4

Authors

Wei Yang

Nian Wang

Xue Shi

Jie Chen

Affiliations

Soon will be listed here.

Abstract

Objective. The aims of this study were to investigate the effects of synchronized dual pulse gastric electrical stimulation (SGES) on gastric motility in different periods for diabetic rats and try to explore the possible mechanisms of the effects. Methods. Forty-six rats were used in the study. Gastric slow waves were recorded at baseline, 7-14-day diabetes and 56-63-day diabetes before and after stimulation and the age-matched control groups. SGES-60 mins and SGES-7 days (60 mins/day) were performed to test the effects on gastric motility and to evaluate glial marker S100B expression in stomach. Results. (1) Gastric emptying was accelerated in 7-14-day diabetes and delayed in 56-63-day diabetes. (2) The S100B expression in 56-63-day diabetes decreased and the ultrastructure changed. (3) The age-associated loss of EGC was observed in 56-63-day control group. (4) SGES was able to not only accelerate gastric emptying but also normalize gastric slow waves. (5) The S100B expression increased after SGES and the ultrastructure of EGC was partially restored. The effect of SGES-7 days was superior to SGES-60 mins. Conclusions. Delayed gastric emptying due to the growth of age may be related to the EGC inactivation. The effects of the SGES on gastric motility may be associated with EGC activation.

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References

Howlett P, Ward A, Duthie H . Gastric emptying after vagotomy. Proc R Soc Med. 1974; 67(9):836-8. PMC: 1645947. View

Soykan I, Sivri B, Sarosiek I, Kiernan B, McCallum R . Demography, clinical characteristics, psychological and abuse profiles, treatment, and long-term follow-up of patients with gastroparesis. Dig Dis Sci. 1998; 43(11):2398-404. DOI: 10.1023/a:1026665728213. View

Brock C, Graversen C, Frokjaer J, Softeland E, Valeriani M, Drewes A . Peripheral and central nervous contribution to gastrointestinal symptoms in diabetic patients with autonomic neuropathy. Eur J Pain. 2012; 17(6):820-31. DOI: 10.1002/j.1532-2149.2012.00254.x. View

Grover M, Farrugia G, Lurken M, Bernard C, Faussone-Pellegrini M, Smyrk T . Cellular changes in diabetic and idiopathic gastroparesis. Gastroenterology. 2011; 140(5):1575-85.e8. PMC: 3081914. DOI: 10.1053/j.gastro.2011.01.046. View

Bortolotti M . Gastric electrical stimulation for gastroparesis: a goal greatly pursued, but not yet attained. World J Gastroenterol. 2011; 17(3):273-82. PMC: 3022286. DOI: 10.3748/wjg.v17.i3.273. View

Nasser Y, Fernandez E, Keenan C, Ho W, Oland L, Tibbles L . Role of enteric glia in intestinal physiology: effects of the gliotoxin fluorocitrate on motor and secretory function. Am J Physiol Gastrointest Liver Physiol. 2006; 291(5):G912-27. DOI: 10.1152/ajpgi.00067.2006. View

Yin J, Chen J, Chen J . Ameliorating effects and mechanisms of electroacupuncture on gastric dysrhythmia, delayed emptying, and impaired accommodation in diabetic rats. Am J Physiol Gastrointest Liver Physiol. 2010; 298(4):G563-70. DOI: 10.1152/ajpgi.00252.2009. View

Turco F, Sarnelli G, Cirillo C, Palumbo I, De Giorgi F, DAlessandro A . Enteroglial-derived S100B protein integrates bacteria-induced Toll-like receptor signalling in human enteric glial cells. Gut. 2013; 63(1):105-15. DOI: 10.1136/gutjnl-2012-302090. View

Song G, Chen J . Synchronized gastric electrical stimulation improves delayed gastric emptying in nonobese mice with diabetic gastroparesis. J Appl Physiol (1985). 2007; 103(5):1560-4. DOI: 10.1152/japplphysiol.00319.2007. View

10.

Broadhead M, Bayguinov P, Okamoto T, Heredia D, Smith T . Ca2+ transients in myenteric glial cells during the colonic migrating motor complex in the isolated murine large intestine. J Physiol. 2011; 590(2):335-50. PMC: 3285069. DOI: 10.1113/jphysiol.2011.219519. View

11.

McCallum R, Chen J, Lin Z, Schirmer B, Williams R, Ross R . Gastric pacing improves emptying and symptoms in patients with gastroparesis. Gastroenterology. 1998; 114(3):456-61. DOI: 10.1016/s0016-5085(98)70528-1. View

12.

Enck P, Rathmann W, Spiekermann M, Czerner D, Tschope D, Ziegler D . Prevalence of gastrointestinal symptoms in diabetic patients and non-diabetic subjects. Z Gastroenterol. 1994; 32(11):637-41. View

13.

Scarpignato C, Capovilla T, Bertaccini G . Action of caerulein on gastric emptying of the conscious rat. Arch Int Pharmacodyn Ther. 1980; 246(2):286-94. View

14.

Bassotti G, Villanacci V, Antonelli E, Morelli A, Salerni B . Enteric glial cells: new players in gastrointestinal motility?. Lab Invest. 2007; 87(7):628-32. DOI: 10.1038/labinvest.3700564. View

15.

Itoh H, Yoneda M, Tamori K, Miyamoto Y, Morikawa A, Eto M . Rapid gastric emptying and pathological changes of vagus nerve in the spontaneously diabetic Chinese hamster. Diabetes Res Clin Pract. 1995; 28(2):89-95. DOI: 10.1016/0168-8227(95)01093-s. View

16.

Aube A, Cabarrocas J, Bauer J, Philippe D, Aubert P, Doulay F . Changes in enteric neurone phenotype and intestinal functions in a transgenic mouse model of enteric glia disruption. Gut. 2005; 55(5):630-7. PMC: 1856141. DOI: 10.1136/gut.2005.067595. View

17.

Gallas S, Fetissov S . Ghrelin, appetite and gastric electrical stimulation. Peptides. 2011; 32(11):2283-9. DOI: 10.1016/j.peptides.2011.05.027. View

18.

Young A . Inhibition of gastric emptying. Adv Pharmacol. 2006; 52:99-121. DOI: 10.1016/S1054-3589(05)52006-4. View

19.

DU F, Wang L, Qian W, Liu S . Loss of enteric neurons accompanied by decreased expression of GDNF and PI3K/Akt pathway in diabetic rats. Neurogastroenterol Motil. 2009; 21(11):1229-e114. DOI: 10.1111/j.1365-2982.2009.01379.x. View

20.

Zhang W, Segura B, Lin T, Hu Y, Mulholland M . Intercellular calcium waves in cultured enteric glia from neonatal guinea pig. Glia. 2003; 42(3):252-62. DOI: 10.1002/glia.10215. View