Derivation, Expansion and Characterization of Clinical Grade Mesenchymal Stem Cells from Umbilical Cord Matrix Using Cord Blood Serum

Overview

Journal Int J Stem Cells

Date 2014 May 24

PMID 24855549

Citations 10

Authors

Khushnuma Cooper

Anish SenMajumdar

Chandra Viswanathan

Affiliations

Soon will be listed here.

Abstract

Background And Objectives: With increasing use of mesenchymal stem cells (MSCs) in regenerative medicine, there is greater awareness towards the need to have clinical grade products. The bovine media currently used allow good expansion to give large number of MSCs of the right quality. This report brings the significance of using cord blood serum (CBS) in the derivation of MSCs from umbilical cord matrix, to help its clinical applicability.

Methods And Results: MSCs isolated from the cord by explant cultures were expanded and characterized by flow cytometry. Cord blood serum while helping expansion, has the ability to preserve the immunophenotype and differentiation potential of the MSCs derived from the umbilical cords.

Conclusions: Our results suggest that MSCs derived and expanded in cord blood serum are better suited for clinical applications.

Citing Articles

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Effects of Physical, Chemical, and Biological Stimulus on h-MSC Expansion and Their Functional Characteristics.

Castilla-Casadiego D, Reyes-Ramos A, Domenech M, Almodovar J Ann Biomed Eng. 2019; 48(2):519-535.

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A Simple Method to Isolate and Expand Human Umbilical Cord Derived Mesenchymal Stem Cells: Using Explant Method and Umbilical Cord Blood Serum.

Hassan G, Kasem I, Soukkarieh C, Aljamali M Int J Stem Cells. 2017; 10(2):184-192.

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Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Contribute to Chondrogenesis in Coculture with Chondrocytes.

Li X, Duan L, Liang Y, Zhu W, Xiong J, Wang D Biomed Res Int. 2016; 2016:3827057.

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Can A, Ulus A, Cinar O, Celikkan F, Simsek E, Akyol M Stem Cell Rev Rep. 2015; 11(5):752-60.

PMID: 26123356 DOI: 10.1007/s12015-015-9601-0.

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