Analysis of Thermophilic Fungal Populations During Phase II of Composting for the Cultivation of Agaricus Subrufescens

Overview

Journal World J Microbiol Biotechnol

Publisher Springer

Specialties Biotechnology
Microbiology

Date 2014 May 23

PMID 24849009

Citations 6

Authors

Thiago Pereira Souza

Simone Cristina Marques

Debora Marques da Silveira E Santos

Eustaquio Souza Dias

Affiliations

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Abstract

The composition and genetic diversity of fungal populations during phase II of compost production for the cultivation of Agaricus subrufescens was determined using culture-dependent and -independent methods on days 3, 6, 10, 12, and 14 of phase II composting. The isolates were morphologically characterized and subsequently analyzed using repetitive extragenic palindromic sequences (rep-PCR), and the intergenic region was sequenced to genetically identify the isolates. Changes on in the filamentous fungi population were analyzed using denaturing gradient gel electrophoresis (DGGE), and the resulting bands were sequenced. The population did not significantly change from day 3 to 10 (2.55 x 10(5) -6 x 10(5) CFU g(-1)), and maximum counts on day 14 of phase II composting (6.92 log CFU g(-1)). In the morphological characterization, Scytalidium thermophilum, Thermomyces lanuginosus, and Thermomyces ibadanensis were the most abundant identified species. The 26 most abundant isolates identified by morphological analysis were characterized using rep-PCR. A significant amount of genetic diversity was detected among the isolates of all three studied species. Based on the DGGE analysis, the diversity of the fungi was reduced during phase II composting, and S. thermophilum was the predominant species identified throughout the entire process. Thus, this study presents the first report of the involvement of T. ibadanensis in the production of compost for Agaricus mushroom cultivation.

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References

Silva C, Azevedo R, Braga C, da Silva R, Dias E, Schwan R . Microbial diversity in a bagasse-based compost prepared for the production of Agaricus brasiliensis. Braz J Microbiol. 2013; 40(3):590-600. PMC: 3768522. DOI: 10.1590/S1517-838220090003000023. View

Sanchez J, Royse D . Scytalidium thermophilum-colonized grain, corncobs and chopped wheat straw substrates for the production of Agaricus bisporus. Bioresour Technol. 2008; 100(4):1670-4. DOI: 10.1016/j.biortech.2008.08.047. View

Jumes F, Lugarini D, Pereira A, de Oliveira A, Christoff A, Linde G . Effects of Agaricus brasiliensis mushroom in Walker-256 tumor-bearing rats. Can J Physiol Pharmacol. 2010; 88(1):21-7. DOI: 10.1139/Y09-111. View

Viikari L, Alapuranen M, Puranen T, Vehmaanpera J, Siika-Aho M . Thermostable enzymes in lignocellulose hydrolysis. Adv Biochem Eng Biotechnol. 2007; 108:121-45. DOI: 10.1007/10_2007_065. View

Maheshwari R, Bharadwaj G, Bhat M . Thermophilic fungi: their physiology and enzymes. Microbiol Mol Biol Rev. 2000; 64(3):461-88. PMC: 99000. DOI: 10.1128/MMBR.64.3.461-488.2000. View

Hetland G, Johnson E, Lyberg T, Bernardshaw S, Tryggestad A, Grinde B . Effects of the medicinal mushroom Agaricus blazei Murill on immunity, infection and cancer. Scand J Immunol. 2008; 68(4):363-70. DOI: 10.1111/j.1365-3083.2008.02156.x. View

Hansgate A, Schloss P, Hay A, Walker L . Molecular characterization of fungal community dynamics in the initial stages of composting. FEMS Microbiol Ecol. 2005; 51(2):209-14. DOI: 10.1016/j.femsec.2004.08.009. View

Wang H, Fan B, Hu Q, Yin Z . Effect of inoculation with Penicillium expansum on the microbial community and maturity of compost. Bioresour Technol. 2011; 102(24):11189-93. DOI: 10.1016/j.biortech.2011.07.044. View

Kerrigan R . Agaricus subrufescens, a cultivated edible and medicinal mushroom, and its synonyms. Mycologia. 2006; 97(1):12-24. DOI: 10.3852/mycologia.97.1.12. View

10.

Mora C, Tittensor D, Adl S, Simpson A, Worm B . How many species are there on Earth and in the ocean?. PLoS Biol. 2011; 9(8):e1001127. PMC: 3160336. DOI: 10.1371/journal.pbio.1001127. View

11.

Straatsma G, Olijnsma T, Gerrits J, Amsing J, Op den Camp H, van Griensven L . Inoculation of Scytalidium thermophilum in Button Mushroom Compost and Its Effect on Yield. Appl Environ Microbiol. 1994; 60(9):3049-54. PMC: 201770. DOI: 10.1128/aem.60.9.3049-3054.1994. View

12.

Sanchez J, Mejia L, Royse D . Pangola grass colonized with Scytalidium thermophilum for production of Agaricus bisporus. Bioresour Technol. 2007; 99(3):655-62. DOI: 10.1016/j.biortech.2006.11.067. View

13.

Cunha Zied D, Pardo-Gimenez A, de Almeida Minhoni M, Villas Boas R, Alvarez-Orti M, Pardo-Gonzalez J . Characterization, feasibility and optimization of Agaricus subrufescens growth based on chemical elements on casing layer. Saudi J Biol Sci. 2013; 19(3):343-7. PMC: 3730930. DOI: 10.1016/j.sjbs.2012.04.002. View

14.

Coello-Castillo M, Sanchez J, Royse D . Production of Agaricus bisporus on substrates pre-colonized by Scytalidium thermophilum and supplemented at casing with protein-rich supplements. Bioresour Technol. 2009; 100(19):4488-92. DOI: 10.1016/j.biortech.2008.10.061. View

15.

Sanchez C . Modern aspects of mushroom culture technology. Appl Microbiol Biotechnol. 2004; 64(6):756-62. DOI: 10.1007/s00253-004-1569-7. View

16.

Basaveswara Rao V, Sastri N, Subba Rao P . Purification and characterization of a thermostable glucoamylase from the thermophilic fungus Thermomyces lanuginosus. Biochem J. 1981; 193(2):379-87. PMC: 1162616. DOI: 10.1042/bj1930379. View

17.

Beeson 4th W, Iavarone A, Hausmann C, Cate J, Marletta M . Extracellular aldonolactonase from Myceliophthora thermophila. Appl Environ Microbiol. 2010; 77(2):650-6. PMC: 3020561. DOI: 10.1128/AEM.01922-10. View

18.

Straatsma G, Samson R, Olijnsma T, Op den Camp H, Gerrits J, van Griensven L . Ecology of Thermophilic Fungi in Mushroom Compost, with Emphasis on Scytalidium thermophilum and Growth Stimulation of Agaricus bisporus Mycelium. Appl Environ Microbiol. 1994; 60(2):454-8. PMC: 201333. DOI: 10.1128/aem.60.2.454-458.1994. View