Potential Cytotoxic Effect of Anilofos by Using Allium Cepa Assay

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2014 May 20

PMID 24838422

Citations 4

Authors

Arzu Ozkara

Dilek Akyil

Yasin Eren

S Feyza Erdogmus

Affiliations

Soon will be listed here.

Abstract

Cytogenetic effects of Anilofos which was widely used in agriculture, was evaluated in Allium cepa root meristematic cells. In the Allium root growth inhibition test EC50 value was determined 50 ppm and 1/2× EC50 (25 ppm), EC50 (50 ppm) and 2 × EC50 (100 ppm) concentrations of Anilofos were applied to onion roots. A negative and positive control were used in the experiment in parallel. According to results mitotic index decreased with increasing the Anilofos concentrations in all application groups and each exposure time, while disturbed anaphase-telophase, choromosome laggard(s), stickiness and anaphase bridge(s) were observed. In anaphase-telophase cells, c-metaphase, disturbed nucleus and binuclear cells were observed in other anomalies. The results were also analyzed statistically by using Dunnett t test (2-tailed) and all concentrations of Anilofos were found significant.

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