Mechanical Stress Stimulates the Osteo/odontoblastic Differentiation of Human Stem Cells from Apical Papilla Via Erk 1/2 and JNK MAPK Pathways

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2014 May 15

PMID 24826377

Citations 17

Authors

Chao Mu

Taohong Lv

Zilu Wang

Shu Ma

Jie Ma

Jin Liu

Jinhua Yu

Jinquan Mu

Affiliations

Soon will be listed here.

Abstract

Background Information: Stem cells from apical papilla (SCAPs) are a potent candidate for the apexogenesis/apexification due to their multiple differentiation capacity. During the orthodontic treatment of developing teeth, SCAPs in vivo are usually subjected to the cyclic stress induced by compression forces. However, it remains unclear whether mechanical stress can affect the proliferation and differentiation of human SCAPs.

Results: Human SCAPs were isolated and stimulated by 200 g mechanical stimuli for 30 min and their proliferation and differentiation capacity were evaluated in vitro at different time points. MTT and FCM results demonstrated that cell proliferation was enhanced, while TEM findings showed the morphological and ultrastructural changes in stress-treated SCAPs. ALP activity and mineralization capacity of stress-treated SCAPs were upregulated . In the meantime, higher odontogenic and osteogenic differentiation were found in stress-treated SCAPs by real-time RT-PCR and Western blot, as indicated by the expression of related markers at both mRNA and protein levels. Moreover, the protein expressions of pJNK and pERK MAPK pathways were upregulated.

Conclusion: Together, these findings suggest that mechanical stress is an important factor affecting the proliferation and differentiation of SCAPs via the activation of ERK and JNK signaling pathway.

Citing Articles

Oscillatory fluid flow enhanced mineralization of human dental pulp cells.

Yortchan W, Puwanun S Front Bioeng Biotechnol. 2025; 13:1500730.

PMID: 39886658 PMC: 11774892. DOI: 10.3389/fbioe.2025.1500730.

The odontoblastic differentiation of dental mesenchymal stem cells: molecular regulation mechanism and related genetic syndromes.

Pan H, Yang Y, Xu H, Jin A, Huang X, Gao X Front Cell Dev Biol. 2023; 11:1174579.

PMID: 37818127 PMC: 10561098. DOI: 10.3389/fcell.2023.1174579.

Subaqueous free-standing 3D cell culture system for ultrafast cell compaction, mechano-inductive immune control, and improving therapeutic angiogenesis.

Im G, Kim Y, Lee T, Bhang S Bioeng Transl Med. 2023; 8(2):e10438.

PMID: 36925707 PMC: 10013761. DOI: 10.1002/btm2.10438.

IL-6 is involved in thoracic ossification of the ligamentum flavum.

Huang A, Shu L, Chen Z, Zhang C PLoS One. 2022; 17(7):e0272357.

PMID: 35905126 PMC: 9337630. DOI: 10.1371/journal.pone.0272357.

Effects of mechanical force on proliferation and apoptosis of stem cells from human exfoliated deciduous teeth.

Liu Q, Qian H, Yu H, Ren F, Fang J, Liu F Clin Oral Investig. 2022; 26(8):5205-5213.

PMID: 35441898 DOI: 10.1007/s00784-022-04488-9.

References

Baumert U, Golan I, Becker B, Hrala B, Redlich M, Roos H . Pressure simulation of orthodontic force in osteoblasts: a pilot study. Orthod Craniofac Res. 2004; 7(1):3-9. DOI: 10.1046/j.1601-6335.2003.00270.x. View

Yan Y, Gong Y, Guo Y, Lv Q, Guo C, Zhuang Y . Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation. PLoS One. 2012; 7(4):e35709. PMC: 3335094. DOI: 10.1371/journal.pone.0035709. View

Hatton J, Pooran M, Li C, Luzzio C, Hughes-Fulford M . A short pulse of mechanical force induces gene expression and growth in MC3T3-E1 osteoblasts via an ERK 1/2 pathway. J Bone Miner Res. 2003; 18(1):58-66. DOI: 10.1359/jbmr.2003.18.1.58. View

Simmons C, Nikolovski J, Thornton A, Matlis S, Mooney D . Mechanical stimulation and mitogen-activated protein kinase signaling independently regulate osteogenic differentiation and mineralization by calcifying vascular cells. J Biomech. 2004; 37(10):1531-41. DOI: 10.1016/j.jbiomech.2004.01.006. View

Sarrafpour B, Swain M, Li Q, Zoellner H . Tooth eruption results from bone remodelling driven by bite forces sensed by soft tissue dental follicles: a finite element analysis. PLoS One. 2013; 8(3):e58803. PMC: 3598949. DOI: 10.1371/journal.pone.0058803. View

Case N, Thomas J, Xie Z, Sen B, Styner M, Rowe D . Mechanical input restrains PPARγ2 expression and action to preserve mesenchymal stem cell multipotentiality. Bone. 2012; 52(1):454-64. PMC: 3535265. DOI: 10.1016/j.bone.2012.08.122. View

Blanco-Gelaz M, Suarez-Alvarez B, Ligero G, Sanchez L, Vidal-Castineira J, Coto E . Endoplasmic reticulum stress signals in defined human embryonic stem cell lines and culture conditions. Stem Cell Rev Rep. 2010; 6(3):462-72. DOI: 10.1007/s12015-010-9135-4. View

Caetano-Lopes J, Canhao H, Fonseca J . Osteoblasts and bone formation. Acta Reumatol Port. 2007; 32(2):103-10. View

Li J, Jiang L, Liao G, Chen G, Liu Y, Wang J . Centrifugal forces within usually-used magnitude elicited a transitory and reversible change in proliferation and gene expression of osteoblastic cells UMR-106. Mol Biol Rep. 2007; 36(2):299-305. DOI: 10.1007/s11033-007-9179-y. View

10.

Qi M, Zou S, Han L, Zhou H, Hu J . Expression of bone-related genes in bone marrow MSCs after cyclic mechanical strain: implications for distraction osteogenesis. Int J Oral Sci. 2010; 1(3):143-50. PMC: 3475587. DOI: 10.4248/IJOS.09021. View

11.

Chueh L, Huang G . Immature teeth with periradicular periodontitis or abscess undergoing apexogenesis: a paradigm shift. J Endod. 2006; 32(12):1205-13. DOI: 10.1016/j.joen.2006.07.010. View

12.

Chen S, Gluhak-Heinrich J, Wang Y, Wu Y, Chuang H, Chen L . Runx2, osx, and dspp in tooth development. J Dent Res. 2009; 88(10):904-9. PMC: 3045537. DOI: 10.1177/0022034509342873. View

13.

Ni P, Fu S, Fan M, Guo G, Shi S, Peng J . Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering. Int J Nanomedicine. 2011; 6:3065-75. PMC: 3235027. DOI: 10.2147/IJN.S25297. View

14.

Yu J, Shi J, Deng Z, Zhuang H, Nie X, Wang R . Cell pellets from dental papillae can reexhibit dental morphogenesis and dentinogenesis. Biochem Biophys Res Commun. 2006; 346(1):116-24. DOI: 10.1016/j.bbrc.2006.05.096. View

15.

Sonoyama W, Liu Y, Fang D, Yamaza T, Seo B, Zhang C . Mesenchymal stem cell-mediated functional tooth regeneration in swine. PLoS One. 2006; 1:e79. PMC: 1762318. DOI: 10.1371/journal.pone.0000079. View

16.

Kang K, Lee S, Lee H, Moon W, Cho D . Effects of combined mechanical stimulation on the proliferation and differentiation of pre-osteoblasts. Exp Mol Med. 2011; 43(6):367-73. PMC: 3128915. DOI: 10.3858/emm.2011.43.6.040. View

17.

Ulsamer A, Ortuno M, Ruiz S, Susperregui A, Osses N, Rosa J . BMP-2 induces Osterix expression through up-regulation of Dlx5 and its phosphorylation by p38. J Biol Chem. 2007; 283(7):3816-26. DOI: 10.1074/jbc.M704724200. View

18.

Sonoyama W, Liu Y, Yamaza T, Tuan R, Wang S, Shi S . Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 2008; 34(2):166-71. PMC: 2714367. DOI: 10.1016/j.joen.2007.11.021. View

19.

Glossop J, Cartmell S . Tensile strain and magnetic particle force application do not induce MAP3K8 and IL-1B differential gene expression in a similar manner to fluid shear stress in human mesenchymal stem cells. J Tissue Eng Regen Med. 2010; 4(7):577-9. DOI: 10.1002/term.293. View

20.

Hie M, Tsukamoto I . Increased expression of the receptor for activation of NF-kappaB and decreased runt-related transcription factor 2 expression in bone of rats with streptozotocin-induced diabetes. Int J Mol Med. 2010; 26(4):611-8. DOI: 10.3892/ijmm_00000506. View