Cortisol Effect on Heat Shock Proteins in the C2C12 and 3T3-L1 Cells

Overview

Journal In Vitro Cell Dev Biol Anim

Publisher Springer

Specialties Biology
Cell Biology

Date 2014 May 15

PMID 24825387

Citations 1

Authors

Sambandam Ravikumar

Pandurangan Muthuraman

Affiliations

Soon will be listed here.

Abstract

The present study was carried out to understand the effect of cortisol on heat shock protein system (Hsps) in the C2C12 and 3T3-L1 cells under co-culture system. Cells were co-cultured by using Transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3T3-L1 cells. Each cell type was grown independently on the Transwell plates. After cell differentiation, inserts containing 3T3-L1 cells were transferred to C2C12 plates and inserts containing C2C12 cells transferred to 3T3-L1 plates. Ten micrograms per microliter of cortisol was added to the medium. Following 72 h of treatment, the cells in the lower wells were harvested for analysis. Heat shock proteins (Hsps) such as Hsp27, Hsp70, and Hsp90 were selected for the analysis. The qRT-PCR results showed the significant increase in the mRNA expression of as Hsp27, Hsp70, and Hsp90. In addition, confocal microscopical investigation showed the cortisol treatment increases Hsps expressions in the mono and co-cultured C2C12 and 3T3-L1 cells. From the results, we concluded that the cortisol increases Hsps expression in the co-cultured C2C12 and 3T3-L1 cells, which is differed from one-dimensional mono-cultured C2C12 and 3T3-L1 cells.

Citing Articles

Selection for environmental variance of litter size in rabbits involves genes in pathways controlling animal resilience.

Casto-Rebollo C, Argente M, Garcia M, Blasco A, Ibanez-Escriche N Genet Sel Evol. 2021; 53(1):59.

PMID: 34256696 PMC: 8276493. DOI: 10.1186/s12711-021-00653-y.

References

Udelsman R, Li D, Stagg C, Gordon C, Kvetnansky R . Adrenergic regulation of adrenal and aortic heat shock protein. Surgery. 1994; 116(2):177-82. View

Paroo Z, Noble E . Isoproterenol potentiates exercise-induction of Hsp70 in cardiac and skeletal muscle. Cell Stress Chaperones. 1999; 4(3):199-204. PMC: 312934. View

Muthuraman P, Ravikumar S, Muthuviveganandavel V, Kim J . Effect of cortisol on calpains in the C2C12 and 3T3-L1 cells. Appl Biochem Biotechnol. 2014; 172(6):3153-62. DOI: 10.1007/s12010-014-0753-1. View

Whitham M, Walker G, Bishop N . Effect of caffeine supplementation on the extracellular heat shock protein 72 response to exercise. J Appl Physiol (1985). 2006; 101(4):1222-7. DOI: 10.1152/japplphysiol.00409.2006. View

Sun X, Zemel M . Calcitriol and calcium regulate cytokine production and adipocyte-macrophage cross-talk. J Nutr Biochem. 2007; 19(6):392-9. DOI: 10.1016/j.jnutbio.2007.05.013. View

Kregel K . Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance. J Appl Physiol (1985). 2002; 92(5):2177-86. DOI: 10.1152/japplphysiol.01267.2001. View

Morimoto R . Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev. 1998; 12(24):3788-96. DOI: 10.1101/gad.12.24.3788. View

Murphy S, Song D, Welsh F, Wilson D, Pastuszko A . The effect of hypoxia and catecholamines on regional expression of heat-shock protein-72 mRNA in neonatal piglet brain. Brain Res. 1996; 727(1-2):145-52. DOI: 10.1016/0006-8993(96)00363-0. View

Muthuraman P . Effect of coculturing on the myogenic and adipogenic marker gene expression. Appl Biochem Biotechnol. 2014; 173(2):571-8. DOI: 10.1007/s12010-014-0866-6. View

10.

Feder M, Hofmann G . Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology. Annu Rev Physiol. 1999; 61:243-82. DOI: 10.1146/annurev.physiol.61.1.243. View

11.

Ananthan J, GOLDBERG A, Voellmy R . Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock genes. Science. 1986; 232(4749):522-4. DOI: 10.1126/science.3083508. View

12.

Pandurangan M, Ravikumar S . Impact of stress hormone on adipogenesis in the 3T3-L1 adipocytes. Cytotechnology. 2013; 66(4):619-24. PMC: 4082784. DOI: 10.1007/s10616-013-9614-y. View

13.

Lindquist S, Craig E . The heat-shock proteins. Annu Rev Genet. 1988; 22:631-77. DOI: 10.1146/annurev.ge.22.120188.003215. View

14.

Currie S, Reddin K, McGinn P, McConnell T, Perry S . Beta-adrenergic stimulation enhances the heat-shock response in fish. Physiol Biochem Zool. 2008; 81(4):414-25. DOI: 10.1086/589095. View

15.

Lacoste A, De Cian M, Cueff A, Poulet S . Noradrenaline and alpha-adrenergic signaling induce the hsp70 gene promoter in mollusc immune cells. J Cell Sci. 2001; 114(Pt 19):3557-64. DOI: 10.1242/jcs.114.19.3557. View

16.

Dodson M, Vierck J, Hossner K, Byrne K, McNamara J . The development and utility of a defined muscle and fat co-culture system. Tissue Cell. 1997; 29(5):517-24. DOI: 10.1016/s0040-8166(97)80052-3. View

17.

Udelsman R, Blake M, Stagg C, Li D, Putney D, Holbrook N . Vascular heat shock protein expression in response to stress. Endocrine and autonomic regulation of this age-dependent response. J Clin Invest. 1993; 91(2):465-73. PMC: 287957. DOI: 10.1172/JCI116224. View

18.

Pfaffl M . A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29(9):e45. PMC: 55695. DOI: 10.1093/nar/29.9.e45. View

19.

Jiang B, Liang P, Deng G, Tu Z, Liu M, Xiao X . Increased stability of Bcl-2 in HSP70-mediated protection against apoptosis induced by oxidative stress. Cell Stress Chaperones. 2010; 16(2):143-52. PMC: 3059790. DOI: 10.1007/s12192-010-0226-6. View