The Identification and Localization of 33 Pea Chloroplast Transcription Initiation Sites

Overview

Journal Curr Genet

Specialty Genetics

Date 1989 Dec 1

PMID 2482136

Citations 16

Authors

N W Woodbury

M Dobres

W F Thompson

Affiliations

Soon will be listed here.

Abstract

We have used a novel approach to produce a comprehensive transcription initiation map of the pea chloroplast genome. Sites were mapped by measuring the ability of DNA probes to protect 5' ends of transcripts that have been capped in vitro. Using this approach, at least 33 probes appear to contain one or more transcription start sites. A more precise location of some of these sites was obtained by hybrid selecting certain of these RNAs and determining their size both before and after RNase treatment. We have found at least one initiation site in front of every chloroplast gene cluster for which appropriate clones were available. In addition, we have found initiation sites within gene clusters previously shown to be co-transcribed. In one such case, we were able to locate a transcription start site for psbC within the coding sequence of psbD.

Citing Articles

Differential accumulation of plastid transcripts encoding photosystem II components in the mesophyll and bundle-sheath cells of monocotyledonous NADP-malic enzyme-type C4 plants.

Westhoff P, Offermann-Steinhard K, Hofer M, Eskins K, Oswald A, Streubel M Planta. 2013; 184(3):377-88.

PMID: 24194156 DOI: 10.1007/BF00195340.

Transcriptional control of plastid gene expression in greening Sorghum seedlings.

Schrubar H, Wanner G, Westhoff P Planta. 2013; 183(1):101-11.

PMID: 24193539 DOI: 10.1007/BF00197573.

Plastid Genes Encoding the Transcription/Translation Apparatus Are Differentially Transcribed Early in Barley (Hordeum vulgare) Chloroplast Development (Evidence for Selective Stabilization of psbA mRNA).

Baumgartner B, Rapp J, Mullet J Plant Physiol. 1993; 101(3):781-791.

PMID: 12231729 PMC: 158691. DOI: 10.1104/pp.101.3.781.

A nuclear mutant of Arabidopsis with impaired stability on distinct transcripts of the plastid psbB, psbD/C, ndhH, and ndhC operons.

Meurer J, Berger A, Westhoff P Plant Cell. 1996; 8(7):1193-207.

PMID: 8768377 PMC: 161203. DOI: 10.1105/tpc.8.7.1193.

A methyl jasmonate-induced shift in the length of the 5' untranslated region impairs translation of the plastid rbcL transcript in barley.

Reinbothe S, Reinbothe C, Heintzen C, Seidenbecher C, Parthier B EMBO J. 1993; 12(4):1505-12.

PMID: 8467800 PMC: 413363. DOI: 10.1002/j.1460-2075.1993.tb05794.x.

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