Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa

Overview

Journal Biochem Res Int

Publisher Wiley

Specialty Biochemistry

Date 2014 May 13

PMID 24818024

Citations 1

Authors

Subir K Nagdas

Eric L McLean

Leea P Richardson

Samir Raychoudhury

Affiliations

Soon will be listed here.

Abstract

Several studies exhibit the presence of Ricinus Communis Agglutinin I (RCA) binding glycocalyx in mammalian spermatozoa. However, the molecular characterization of RCA binding glycocalyx in sperm membranes and its mechanism of action are poorly understood. The objective of the study was to identify and to characterize RCA binding glycoprotein of the bovine sperm plasma membranes (PM). Lectin blots of caput and cauda sperm PM revealed a 38 kDa polypeptide exhibiting the highest affinity to RCA among the several major RCA binding polypeptides. The 38 kDa RCA binding polypeptide of cauda sperm PM was purified and exhibited a charge train of three distinct spots with isoelectric points (pH 5.3 and 5.8). Proteomic identification yielded ten peptides that matched the sequence of Testis Expressed 101 protein (TEX101). Western blots data revealed that bovine sperm TEX101 is present in both testicular and epididymal sperm PM fractions. The native TEX101 polypeptide contains ~17 kDa N-linked oligosaccharides and the polypeptide is anchored to sperm membrane via a glycosylphosphatidylinositol lipid linkage. Immunofluorescence staining of sperm with anti-TEX101 demonstrated that the polypeptide is localized at the head of cauda sperm. Our biochemical results provide evidence on the presence of TEX101 in bovine epididymal sperm plasma membranes and may have a potential role in sperm-egg interaction.

Citing Articles

Comparative proteomic analysis of seminal plasma exosomes in buffalo with high and low sperm motility.

Yu K, Xiao K, Sun Q, Liu R, Huang L, Zhang P BMC Genomics. 2023; 24(1):8.

PMID: 36624393 PMC: 9830767. DOI: 10.1186/s12864-022-09106-2.

References

MEDEIROS C, Parrish J . Changes in lectin binding to bovine sperm during heparin-induced capacitation. Mol Reprod Dev. 1996; 44(4):525-32. DOI: 10.1002/(SICI)1098-2795(199608)44:4<525::AID-MRD12>3.0.CO;2-X. View

Takayama T, Mishima T, Mori M, Ishikawa T, Takizawa T, Goto T . TEX101 is shed from the surface of sperm located in the caput epididymidis of the mouse. Zygote. 2006; 13(4):325-33. DOI: 10.1017/S0967199405003394. View

Yoshitake H, Yanagida M, Maruyama M, Takamori K, Hasegawa A, Araki Y . Molecular characterization and expression of dipeptidase 3, a testis-specific membrane-bound dipeptidase: complex formation with TEX101, a germ-cell-specific antigen in the mouse testis. J Reprod Immunol. 2011; 90(2):202-13. DOI: 10.1016/j.jri.2011.04.010. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Miranda P, Allaire A, Sosnik J, Visconti P . Localization of low-density detergent-resistant membrane proteins in intact and acrosome-reacted mouse sperm. Biol Reprod. 2009; 80(5):897-904. PMC: 2804839. DOI: 10.1095/biolreprod.108.075242. View

Rankin T, Holland M, ORGEBIN-CRIST M . Lectin binding characteristics of mouse epididymal fluid and sperm extracts. Gamete Res. 1989; 24(4):439-51. DOI: 10.1002/mrd.1120240410. View

Kurita A, Takizawa T, Takayama T, Totsukawa K, Matsubara S, Shibahara H . Identification, cloning, and initial characterization of a novel mouse testicular germ cell-specific antigen. Biol Reprod. 2001; 64(3):935-45. DOI: 10.1095/biolreprod64.3.935. View

Sleight S, Miranda P, Plaskett N, Maier B, Lysiak J, Scrable H . Isolation and proteomic analysis of mouse sperm detergent-resistant membrane fractions: evidence for dissociation of lipid rafts during capacitation. Biol Reprod. 2005; 73(4):721-9. DOI: 10.1095/biolreprod.105.041533. View

Lee S, Ahuja K . An investigation using lectins of glycocomponents of mouse spermatozoa during capacitation and sperm-zona binding. J Reprod Fertil. 1987; 80(1):65-74. DOI: 10.1530/jrf.0.0800065. View

10.

Fairbanks G, Steck T, Wallach D . Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971; 10(13):2606-17. DOI: 10.1021/bi00789a030. View

11.

Yoshitake H, Tsukamoto H, Maruyama-Fukushima M, Takamori K, Ogawa H, Araki Y . TEX101, a germ cell-marker glycoprotein, is associated with lymphocyte antigen 6 complex locus k within the mouse testis. Biochem Biophys Res Commun. 2008; 372(2):277-82. DOI: 10.1016/j.bbrc.2008.05.088. View

12.

Fujihara Y, Tokuhiro K, Muro Y, Kondoh G, Araki Y, Ikawa M . Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa. Proc Natl Acad Sci U S A. 2013; 110(20):8111-6. PMC: 3657793. DOI: 10.1073/pnas.1222166110. View

13.

Jin H, Yoshitake H, Tsukamoto H, Takahashi M, Mori M, Takizawa T . Molecular characterization of a germ-cell-specific antigen, TEX101, from mouse testis. Zygote. 2006; 14(3):201-8. DOI: 10.1017/S0967199406003753. View

14.

Magargee S, Kunze E, Hammerstedt R . Changes in lectin-binding features of ram sperm surfaces associated with epididymal maturation and ejaculation. Biol Reprod. 1988; 38(3):667-85. DOI: 10.1095/biolreprod38.3.667. View

15.

Koehler J . Lectins as probes of the spermatozoon surface. Arch Androl. 1981; 6(3):197-217. DOI: 10.3109/01485018108987531. View

16.

Kondoh G, Tojo H, Nakatani Y, Komazawa N, Murata C, Yamagata K . Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization. Nat Med. 2005; 11(2):160-6. PMC: 7095966. DOI: 10.1038/nm1179. View

17.

Aitken R, Nixon B, Lin M, Koppers A, Lee Y, Baker M . Proteomic changes in mammalian spermatozoa during epididymal maturation. Asian J Androl. 2007; 9(4):554-64. DOI: 10.1111/j.1745-7262.2007.00280.x. View

18.

Nagdas S, Buchanan T, McCaskill S, Mackey J, Alvarez G, Raychoudhury S . Isolation of a calcium-binding protein of the acrosomal membrane of bovine spermatozoa. Int J Biochem Cell Biol. 2013; 45(4):876-84. PMC: 3721343. DOI: 10.1016/j.biocel.2013.01.016. View

19.

Maruyama M, Yoshitake H, Tsukamoto H, Takamori K, Araki Y . Molecular expression of Ly6k, a putative glycosylphosphatidyl-inositol-anchored membrane protein on the mouse testicular germ cells. Biochem Biophys Res Commun. 2010; 402(1):75-81. DOI: 10.1016/j.bbrc.2010.09.117. View

20.

Nagdas S, Winfrey V, Olson G . Identification of ras and its downstream signaling elements and their potential role in hamster sperm motility. Biol Reprod. 2002; 67(4):1058-66. DOI: 10.1095/biolreprod67.4.1058. View