Protein Heterogeneity of Francisella Tularensis: Detection of Proteins with Antigenic Determinants

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 1989 Jan 1

PMID 2481633

Citations 1

Authors

J Stulik

J Cerna

H Kovarova

A Macela

Affiliations

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Abstract

The autolyzates of three different strains of Francisella tularensis 15L, 130 and SCHU were tested for their immunogenic potential and protein heterogeneity. The autolyzates induce the production of specific antibodies, the delayed type of hypersensitivity, and some degree of protection against European virulent strain 130. This material (as antigen) was especially suitable for ELISA. When the autolyzates were subjected to SDS gradient PAGE, a variety of polypeptides were distinguished. The composition of polypeptides from all three strains on SDS-PAGE was almost identical. After the detection of antigenic determinants by Western blotting more than twenty bands appeared. The visualization with polyclonal antisera against live laboratory strain 15L and against autolyzates 15L, 130 and SCHU revealed differences in the composition of the antigenic determinants of these strains.

Citing Articles

The production of oxygen metabolites and their possible regulatory role in the course of tularemia infection.

Kovarova H, Macela A, Stulik J Folia Microbiol (Praha). 1990; 35(5):413-22.

PMID: 1702745 DOI: 10.1007/BF02821410.

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