Overcoming Multidrug Resistance in Chinese Hamster Ovary Cells in Vitro by Cyclosporin A (Sandimmune) and Non-immunosuppressive Derivatives

Overview

Journal Br J Cancer

Specialty Oncology

Date 1989 Dec 1

PMID 2481487

Citations 11

Authors

C Gaveriaux

D Boesch

J J Boelsterli

P Bollinger

M K Eberle

P Hiestand

T Payne

R Traber

R Wenger

F Loor

Affiliations

Soon will be listed here.

Abstract

Cyclosporin A (Sandimmune) increased the in vitro susceptibility of 'parental' and 'multidrug-resistant' (MDR) chinese hamster ovary (CHO) cell lines to three anti-tumour drugs: colchicine, daunomycin, and vincristine. Several immunosuppressive or non-immunosuppressive derivatives of cyclosporin (Cs) were compared for their ability to sensitise both parental and MDR cells to chemotherapeutic agents. Although 5-10-fold increases of sensitivity to anti-tumour drugs could be obtained for cells of the parental line with several Cs-derivatives, the largest 'gains' of sensitivity (chemosensitisation) were obtained for the cells of the MDR line and with only some of the Cs derivatives. The MDR cells employed displayed the typical MDR phenotype. However, we found no correlation between the immunosuppressive activity of Cs derivatives and their capacity to reverse MDR and all four possible combinations of these two activities could indeed be shown among the tested Cs derivatives. This study demonstrates for the first time that some immunosuppressive Cs can be devoid of chemosensitising activity.

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References

Ling V, Thompson L . Reduced permeability in CHO cells as a mechanism of resistance to colchicine. J Cell Physiol. 1974; 83(1):103-16. DOI: 10.1002/jcp.1040830114. View

Gottesman M, Pastan I . Resistance to multiple chemotherapeutic agents in human cancer cells. Trends Pharmacol Sci. 1988; 9(2):54-8. DOI: 10.1016/0165-6147(88)90117-4. View

Koponen M, Grieder A, Loor F . The effects of cyclosporins on the cell cycle of t-lymphoid cell lines. Exp Cell Res. 1982; 140(2):237-50. DOI: 10.1016/0014-4827(82)90111-2. View

Kartner N, Riordan J, Ling V . Cell surface P-glycoprotein associated with multidrug resistance in mammalian cell lines. Science. 1983; 221(4617):1285-8. DOI: 10.1126/science.6137059. View

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

Handschumacher R, Harding M, Rice J, Drugge R, Speicher D . Cyclophilin: a specific cytosolic binding protein for cyclosporin A. Science. 1984; 226(4674):544-7. DOI: 10.1126/science.6238408. View

Beck W, Cirtain M, Look A, Ashmun R . Reversal of Vinca alkaloid resistance but not multiple drug resistance in human leukemic cells by verapamil. Cancer Res. 1986; 46(2):778-84. View

Slater L, Sweet P, Stupecky M, Gupta S . Cyclosporin A reverses vincristine and daunorubicin resistance in acute lymphatic leukemia in vitro. J Clin Invest. 1986; 77(4):1405-8. PMC: 424510. DOI: 10.1172/JCI112450. View

Slater L, Sweet P, Stupecky M, Wetzel M, Gupta S . Cyclosporin A corrects daunorubicin resistance in Ehrlich ascites carcinoma. Br J Cancer. 1986; 54(2):235-8. PMC: 2001522. DOI: 10.1038/bjc.1986.167. View

10.

van der Bliek A, van der Velde-Koerts T, Ling V, Borst P . Overexpression and amplification of five genes in a multidrug-resistant Chinese hamster ovary cell line. Mol Cell Biol. 1986; 6(5):1671-8. PMC: 367694. DOI: 10.1128/mcb.6.5.1671-1678.1986. View

11.

Stark G . Cancer chemotherapy. Progress in understanding multidrug resistance. Nature. 1986; 324(6096):407-8. DOI: 10.1038/324407a0. View

12.

Osieka R, Seeber S, Pannenbacker R, Soll D, Glatte P, SCHMIDT C . Enhancement of etoposide-induced cytotoxicity by cyclosporin A. Cancer Chemother Pharmacol. 1986; 18(3):198-202. DOI: 10.1007/BF00273385. View

13.

Ueda K, Cardarelli C, Gottesman M, Pastan I . Expression of a full-length cDNA for the human "MDR1" gene confers resistance to colchicine, doxorubicin, and vinblastine. Proc Natl Acad Sci U S A. 1987; 84(9):3004-8. PMC: 304789. DOI: 10.1073/pnas.84.9.3004. View

14.

Gerlach J, Kartner N, Bell D, Ling V . Multidrug resistance. Cancer Surv. 1986; 5(1):25-46. View

15.

Twentyman P, Fox N, White D . Cyclosporin A and its analogues as modifiers of adriamycin and vincristine resistance in a multi-drug resistant human lung cancer cell line. Br J Cancer. 1987; 56(1):55-7. PMC: 2001675. DOI: 10.1038/bjc.1987.153. View

16.

Quesniaux V, SCHREIER M, Wenger R, Hiestand P, Harding M, Van Regenmortel M . Cyclophilin binds to the region of cyclosporine involved in its immunosuppressive activity. Eur J Immunol. 1987; 17(9):1359-65. DOI: 10.1002/eji.1830170921. View

17.

Meador J, Sweet P, Stupecky M, Wetzel M, Murray S, Gupta S . Enhancement by cyclosporin A of daunorubicin efficacy in Ehrlich ascites carcinoma and murine hepatoma 129. Cancer Res. 1987; 47(23):6216-9. View

18.

Alley M, Scudiero D, Monks A, Hursey M, Czerwinski M, Fine D . Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 1988; 48(3):589-601. View

19.

Inaba M, Maruyama E . Reversal of resistance to vincristine in P388 leukemia by various polycyclic clinical drugs, with a special emphasis on quinacrine. Cancer Res. 1988; 48(8):2064-7. View

20.

Scheper R, Bulte J, Brakkee J, Quak J, van der Schoot E, Balm A . Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multi-drug-resistance. Int J Cancer. 1988; 42(3):389-94. DOI: 10.1002/ijc.2910420314. View