The A-type Receptor for Platelet-derived Growth Factor Mediates Protein Tyrosine Phosphorylation, Receptor Transmodulation and a Mitogenic Response

Overview

Journal Biochem J

Specialty Biochemistry

Date 1989 Nov 15

PMID 2481443

Citations 1

Authors

A Hammacher

M Nister

C H Heldin

Affiliations

Soon will be listed here.

Abstract

A clonal human glioma cell line, U-343 MGa 31L, which expresses the A-type but not the B-type receptor for platelet-derived growth factor (PDGF), was used in a functional study of the A-type receptor. PDGF-AA induced, in a dose- and time-dependent manner, phosphorylation on tyrosine residues of the receptor in metabolically labelled cells. The optimal dose was around 30 ng/ml; at 100 ng/ml, phosphorylation was maximal at 15 min and had almost returned to the control level after 60 min. The phosphorylation on tyrosine residues of the PDGF A-type receptor was stimulated by PDGF-AA, PDGF-AB and PDGF-BB; these isoforms also stimulated [3H]thymidine incorporation into U-343 MGa 31L cells. In addition, activation of the A-type PDGF receptor induced transmodulation of the epidermal growth factor receptor.

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Platelet-derived growth factor activates phospholipase D and chemotactic responses in vascular smooth muscle cells.

Welsh C, Schmeichel K, McBride K In Vitro Cell Dev Biol. 1991; 27A(5):425-31.

PMID: 2071544 DOI: 10.1007/BF02630963.

References

McKeehan W, McKeehan K, Hammond S, HAM R . Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein. In Vitro. 1977; 13(7):399-416. DOI: 10.1007/BF02615100. View

Nister M, Hammacher A, Mellstrom K, Siegbahn A, Ronnstrand L, Westermark B . A glioma-derived PDGF A chain homodimer has different functional activities from a PDGF AB heterodimer purified from human platelets. Cell. 1988; 52(6):791-9. DOI: 10.1016/0092-8674(88)90421-7. View

Heldin C, Wasteson A, Westermark B . Platelet-derived growth factor. Mol Cell Endocrinol. 1985; 39(3):169-87. DOI: 10.1016/0303-7207(85)90061-9. View

Escobedo J, Williams L . A PDGF receptor domain essential for mitogenesis but not for many other responses to PDGF. Nature. 1988; 335(6185):85-7. DOI: 10.1038/335085a0. View

ROBBINS K, Antoniades H, Devare S, Hunkapiller M, Aaronson S . Structural and immunological similarities between simian sarcoma virus gene product(s) and human platelet-derived growth factor. Nature. 1983; 305(5935):605-8. DOI: 10.1038/305605a0. View

Daniel T, Tremble P, Frackelton Jr A, Williams L . Purification of the platelet-derived growth factor receptor by using an anti-phosphotyrosine antibody. Proc Natl Acad Sci U S A. 1985; 82(9):2684-7. PMC: 397629. DOI: 10.1073/pnas.82.9.2684. View

Bowen-Pope D, DiCorleto P, Ross R . Interactions between the receptors for platelet-derived growth factor and epidermal growth factor. J Cell Biol. 1983; 96(3):679-83. PMC: 2112417. DOI: 10.1083/jcb.96.3.679. View

Betsholtz C, Westermark B . Growth factor-induced proliferation of human fibroblasts in serum-free culture depends on cell density and extracellular calcium concentration. J Cell Physiol. 1984; 118(2):203-10. DOI: 10.1002/jcp.1041180213. View

Yarden Y, Escobedo J, Kuang W, Yang-Feng T, Daniel T, Tremble P . Structure of the receptor for platelet-derived growth factor helps define a family of closely related growth factor receptors. Nature. 1986; 323(6085):226-32. DOI: 10.1038/323226a0. View

10.

Keating M, Williams L . Processing of the platelet-derived growth factor receptor. Biosynthetic and degradation studies using anti-receptor antibodies. J Biol Chem. 1987; 262(16):7932-7. View

11.

Hammacher A, Nister M, Westermark B, Heldin C . A human glioma cell line secretes three structurally and functionally different dimeric forms of platelet-derived growth factor. Eur J Biochem. 1988; 176(1):179-86. DOI: 10.1111/j.1432-1033.1988.tb14266.x. View

12.

Johnsson A, Heldin C, Westermark B, Wasteson A . Platelet-derived growth factor: identification of constituent polypeptide chains. Biochem Biophys Res Commun. 1982; 104(1):66-74. DOI: 10.1016/0006-291x(82)91941-6. View

13.

Stroobant P, Waterfield M . Purification and properties of porcine platelet-derived growth factor. EMBO J. 1984; 3(12):2963-7. PMC: 557798. DOI: 10.1002/j.1460-2075.1984.tb02241.x. View

14.

Heldin C, Wasteson A, Westermark B . Interaction of platelet-derived growth factor with its fibroblast receptor. Demonstration of ligand degradation and receptor modulation. J Biol Chem. 1982; 257(8):4216-21. View

15.

Ek B, Heldin C . Use of an antiserum against phosphotyrosine for the identification of phosphorylated components in human fibroblasts stimulated by platelet-derived growth factor. J Biol Chem. 1984; 259(17):11145-52. View

16.

Claesson-Welsh L, Eriksson A, Moren A, Severinsson L, Ek B, Ostman A . cDNA cloning and expression of a human platelet-derived growth factor (PDGF) receptor specific for B-chain-containing PDGF molecules. Mol Cell Biol. 1988; 8(8):3476-86. PMC: 363585. DOI: 10.1128/mcb.8.8.3476-3486.1988. View

17.

Heldin C, Johnsson A, Wennergren S, Wernstedt C, Betsholtz C, Westermark B . A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature. 1986; 319(6053):511-4. DOI: 10.1038/319511a0. View

18.

Severinsson L, Claesson-Welsh L, Heldin C . A B-type PDGF receptor lacking most of the intracellular domain escapes degradation after ligand binding. Eur J Biochem. 1989; 182(3):679-86. DOI: 10.1111/j.1432-1033.1989.tb14879.x. View

19.

Hunter W, GREENWOOD F . Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962; 194:495-6. DOI: 10.1038/194495a0. View

20.

Bishayee S, Ross A, Womer R, Scher C . Purified human platelet-derived growth factor receptor has ligand-stimulated tyrosine kinase activity. Proc Natl Acad Sci U S A. 1986; 83(18):6756-60. PMC: 386588. DOI: 10.1073/pnas.83.18.6756. View