Understanding and Manipulating Plant Lipid Composition: Metabolic Engineering Leads the Way

Overview

Journal Curr Opin Plant Biol

Specialty Biology

Date 2014 May 10

PMID 24809765

Citations 36

Authors

Johnathan A Napier

Richard P Haslam

Frederic Beaudoin

Edgar B Cahoon

Affiliations

Soon will be listed here.

Abstract

The manipulation of plant seed oil composition so as to deliver enhanced fatty acid compositions suitable for feed or fuel has long been a goal of metabolic engineers. Recent advances in our understanding of the flux of acyl-changes through different key metabolic pools such as phosphatidylcholine and diacylglycerol have allowed for more targeted interventions. When combined in iterative fashion with further lipidomic analyses, significant breakthroughs in our capacity to generate plants with novel oils have been achieved. Collectively these studies, working at the interface between metabolic engineering and synthetic biology, demonstrate the positive fundamental and applied outcomes derived from such research.

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References

Ruiz-Lopez N, Sayanova O, Napier J, Haslam R . Metabolic engineering of the omega-3 long chain polyunsaturated fatty acid biosynthetic pathway into transgenic plants. J Exp Bot. 2012; 63(7):2397-410. DOI: 10.1093/jxb/err454. View

Vanhercke T, El Tahchy A, Shrestha P, Zhou X, Singh S, Petrie J . Synergistic effect of WRI1 and DGAT1 coexpression on triacylglycerol biosynthesis in plants. FEBS Lett. 2013; 587(4):364-9. DOI: 10.1016/j.febslet.2012.12.018. View

Cernac A, Benning C . WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis. Plant J. 2004; 40(4):575-85. DOI: 10.1111/j.1365-313X.2004.02235.x. View

Horn P, James C, Gidda S, Kilaru A, Dyer J, Mullen R . Identification of a new class of lipid droplet-associated proteins in plants. Plant Physiol. 2013; 162(4):1926-36. PMC: 3729771. DOI: 10.1104/pp.113.222455. View

Nguyen H, Silva J, Podicheti R, Macrander J, Yang W, Nazarenus T . Camelina seed transcriptome: a tool for meal and oil improvement and translational research. Plant Biotechnol J. 2013; 11(6):759-69. DOI: 10.1111/pbi.12068. View

Bates P, Stymne S, Ohlrogge J . Biochemical pathways in seed oil synthesis. Curr Opin Plant Biol. 2013; 16(3):358-64. DOI: 10.1016/j.pbi.2013.02.015. View

Sayanova O, Ruiz-Lopez N, Haslam R, Napier J . The role of Δ6-desaturase acyl-carrier specificity in the efficient synthesis of long-chain polyunsaturated fatty acids in transgenic plants. Plant Biotechnol J. 2011; 10(2):195-206. DOI: 10.1111/j.1467-7652.2011.00653.x. View

Andrianov V, Borisjuk N, Pogrebnyak N, Brinker A, Dixon J, Spitsin S . Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass. Plant Biotechnol J. 2010; 8(3):277-87. DOI: 10.1111/j.1467-7652.2009.00458.x. View

Guan R, Lager I, Li X, Stymne S, Zhu L . Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid Crambe abyssinica. Plant Biotechnol J. 2013; 12(2):193-203. PMC: 4286110. DOI: 10.1111/pbi.12128. View

10.

Slocombe S, Cornah J, Pinfield-Wells H, Soady K, Zhang Q, Gilday A . Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways. Plant Biotechnol J. 2009; 7(7):694-703. DOI: 10.1111/j.1467-7652.2009.00435.x. View

11.

Haslam R, Ruiz-Lopez N, Eastmond P, Moloney M, Sayanova O, Napier J . The modification of plant oil composition via metabolic engineering--better nutrition by design. Plant Biotechnol J. 2012; 11(2):157-68. DOI: 10.1111/pbi.12012. View

12.

James C, Horn P, Case C, Gidda S, Zhang D, Mullen R . Disruption of the Arabidopsis CGI-58 homologue produces Chanarin-Dorfman-like lipid droplet accumulation in plants. Proc Natl Acad Sci U S A. 2010; 107(41):17833-8. PMC: 2955100. DOI: 10.1073/pnas.0911359107. View

13.

Ruiz-Lopez N, Haslam R, Usher S, Napier J, Sayanova O . Reconstitution of EPA and DHA biosynthesis in arabidopsis: iterative metabolic engineering for the synthesis of n-3 LC-PUFAs in transgenic plants. Metab Eng. 2013; 17:30-41. PMC: 3650579. DOI: 10.1016/j.ymben.2013.03.001. View

14.

Ruiz-Lopez N, Haslam R, Venegas-Caleron M, Li T, Bauer J, Napier J . Enhancing the accumulation of omega-3 long chain polyunsaturated fatty acids in transgenic Arabidopsis thaliana via iterative metabolic engineering and genetic crossing. Transgenic Res. 2012; 21(6):1233-43. DOI: 10.1007/s11248-012-9596-0. View

15.

Petrie J, Shrestha P, Zhou X, Mansour M, Liu Q, Belide S . Metabolic engineering plant seeds with fish oil-like levels of DHA. PLoS One. 2012; 7(11):e49165. PMC: 3492320. DOI: 10.1371/journal.pone.0049165. View

16.

Burgal J, Shockey J, Lu C, Dyer J, Larson T, Graham I . Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil. Plant Biotechnol J. 2008; 6(8):819-31. PMC: 2908398. DOI: 10.1111/j.1467-7652.2008.00361.x. View

17.

Li X, Fan J, Gruber J, Guan R, Frentzen M, Zhu L . Efficient selection and evaluation of transgenic lines of Crambe abyssinica. Front Plant Sci. 2013; 4:162. PMC: 3664313. DOI: 10.3389/fpls.2013.00162. View

18.

Sanjaya , Miller R, Durrett T, Kosma D, Lydic T, Muthan B . Altered lipid composition and enhanced nutritional value of Arabidopsis leaves following introduction of an algal diacylglycerol acyltransferase 2. Plant Cell. 2013; 25(2):677-93. PMC: 3608786. DOI: 10.1105/tpc.112.104752. View

19.

Yu X, Prakash R, Sweet M, Shanklin J . Coexpressing Escherichia coli cyclopropane synthase with Sterculia foetida Lysophosphatidic acid acyltransferase enhances cyclopropane fatty acid accumulation. Plant Physiol. 2013; 164(1):455-65. PMC: 3875821. DOI: 10.1104/pp.113.230953. View

20.

Hoffmann M, Wagner M, Abbadi A, Fulda M, Feussner I . Metabolic engineering of omega3-very long chain polyunsaturated fatty acid production by an exclusively acyl-CoA-dependent pathway. J Biol Chem. 2008; 283(33):22352-62. DOI: 10.1074/jbc.M802377200. View