Cellular Activation and Intracellular HCV Load in Peripheral Blood Monocytes Isolated from HCV Monoinfected and HIV-HCV Coinfected Patients

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2014 May 10

PMID 24809719

Citations 3

Authors

Isabelle Dichamp

Wasim Abbas

Amit Kumar

Vincent Di Martino

Georges Herbein

Affiliations

Soon will be listed here.

Abstract

Background: During HCV infection, the activation status of peripheral blood monocytes and its impact on HCV replication are poorly understood. We hypothesized that a modified activation of peripheral blood monocytes in HIV-HCV coinfected compared to HCV monoinfected patients may contribute to different monocytes reservoirs of HCV replication.

Methods: We performed a case-control analysis involving HCV-infected patients with and without HIV coinfection. In peripheral blood mononuclear cells (PBMCs), peripheral blood lymphocytes (PBLs) and peripheral blood monocytes isolated from HCV monoinfected and HIV-HCV coinfected patients, intracellular HCV load and a marker of cellular activation, nuclear factor-kappaB (NF-κB) activation, were quantified using intracellular detection of HCV-core protein and electrophoretic mobility shift assay, respectively.

Results: Intracellular HCV loads were higher in monocytes isolated from HIV-HCV coinfected patients than in those of monoinfected patients. Among PBMCs isolated from HIV-HCV coinfected patients, intracellular HCV loads were higher in monocytes compared to PBLs. Cellular activation as measured by NF-κB activation was higher in monocytes isolated from HIV-HCV coinfected patients than in those of monoinfected patients.

Conclusions: Our results reveal the peripheral blood monocytes as an important extrahepatic reservoir for HCV in HIV-HCV coinfected patients and indicate a potential association between the activation state of monocytes and the size of the HCV reservoir in HIV-HCV coinfected patients.

Citing Articles

T Cell Distribution in Relation to HIV/HBV/HCV Coinfections and Intravenous Drug Use.

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Human Cathelicidin Compensates for the Role of Apolipoproteins in Hepatitis C Virus Infectious Particle Formation.

Puig-Basagoiti F, Fukuhara T, Tamura T, Ono C, Uemura K, Kawachi Y J Virol. 2016; 90(19):8464-77.

PMID: 27440892 PMC: 5021414. DOI: 10.1128/JVI.00471-16.

Eradication of HIV-1 from the macrophage reservoir: an uncertain goal?.

Abbas W, Tariq M, Iqbal M, Kumar A, Herbein G Viruses. 2015; 7(4):1578-98.

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