Aligned Collagen-GAG Matrix As a 3D Substrate for Schwann Cell Migration and Dendrimer-based Gene Delivery

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2014 May 8

PMID 24801062

Citations 3

Authors

Antos Shakhbazau

Simon J Archibald

Dzmitry Shcharbin

Maria Bryszewska

Rajiv Midha

Affiliations

Soon will be listed here.

Abstract

The development of artificial off-the-shelf conduits that facilitate effective nerve regeneration and recovery after repair of traumatic nerve injury gaps is of fundamental importance. Collagen-glycosaminoglycan (GAG) matrix mimicking Schwann cell (SC) basal lamina has been proposed as a suitable and biologically rational substrate for nerve regeneration. In the present study, we have focused on the permissiveness of this matrix type for SC migration and repopulation, as these events play an essential role in nerve remodeling. We have also demonstrated that SCs cultured within collagen-GAG matrix are compatible with non-viral dendrimer-based gene delivery, that may allow conditioning of matrix-embedded cells for future gene therapy applications.

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