The Drosophila Hairy Protein Acts in Both Segmentation and Bristle Patterning and Shows Homology to N-myc

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1989 Oct 1

PMID 2479541

Citations 66

Authors

C A Rushlow

A Hogan

S M Pinchin

K M Howe

M Lardelli

D Ish-Horowicz

Affiliations

Soon will be listed here.

Abstract

The Drosophila segmentation gene, hairy (h), acts to regulate embryonic segmentation and bristle pattern. We present the DNA sequence of the h gene and of h cDNAs, thereby deducing the organization of the h transcripts. The h gene encodes a 337 amino acid protein that acts in both embryonic segmentation and adult bristle patterning. The h protein includes a domain that shows extensive similarity to a domain of the proto-oncogene N-myc that may be involved in DNA binding and/or protein dimerization. We discuss mechanisms of h action as a transcriptional regulator.

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References

Wharton K, Yedvobnick B, Finnerty V, Artavanis-Tsakonas S . opa: a novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogaster. Cell. 1985; 40(1):55-62. DOI: 10.1016/0092-8674(85)90308-3. View

Klambt C, Knust E, TIETZE K, Campos-Ortega J . Closely related transcripts encoded by the neurogenic gene complex enhancer of split of Drosophila melanogaster. EMBO J. 1989; 8(1):203-10. PMC: 400790. DOI: 10.1002/j.1460-2075.1989.tb03365.x. View

Bopp D, Burri M, Baumgartner S, Frigerio G, Noll M . Conservation of a large protein domain in the segmentation gene paired and in functionally related genes of Drosophila. Cell. 1986; 47(6):1033-40. DOI: 10.1016/0092-8674(86)90818-4. View

Holmgren R . Cloning sequences from the hairy gene of Drosophila. EMBO J. 1984; 3(3):569-73. PMC: 557389. DOI: 10.1002/j.1460-2075.1984.tb01849.x. View

Cavener D . Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates. Nucleic Acids Res. 1987; 15(4):1353-61. PMC: 340553. DOI: 10.1093/nar/15.4.1353. View

Benoist C, OHare K, Breathnach R, Chambon P . The ovalbumin gene-sequence of putative control regions. Nucleic Acids Res. 1980; 8(1):127-42. PMC: 327247. DOI: 10.1093/nar/8.1.127. View

Pearson W, Lipman D . Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A. 1988; 85(8):2444-8. PMC: 280013. DOI: 10.1073/pnas.85.8.2444. View

Sassone-Corsi P, Ransone L, Lamph W, Verma I . Direct interaction between fos and jun nuclear oncoproteins: role of the 'leucine zipper' domain. Nature. 1988; 336(6200):692-5. DOI: 10.1038/336692a0. View

Laughon A, Scott M . Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins. Nature. 1984; 310(5972):25-31. DOI: 10.1038/310025a0. View

10.

Moore C, Sharp P . Site-specific polyadenylation in a cell-free reaction. Cell. 1984; 36(3):581-91. DOI: 10.1016/0092-8674(84)90337-4. View

11.

Ish-Horowicz D, Howard K, Pinchin S, Ingham P . Molecular and genetic analysis of the hairy locus in Drosophila. Cold Spring Harb Symp Quant Biol. 1985; 50:135-44. DOI: 10.1101/sqb.1985.050.01.019. View

12.

Marshall R . Glycoproteins. Annu Rev Biochem. 1972; 41:673-702. DOI: 10.1146/annurev.bi.41.070172.003325. View

13.

Halazonetis T, Georgopoulos K, Greenberg M, Leder P . c-Jun dimerizes with itself and with c-Fos, forming complexes of different DNA binding affinities. Cell. 1988; 55(5):917-24. DOI: 10.1016/0092-8674(88)90147-x. View

14.

Walker J, Saraste M, Runswick M, Gay N . Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1982; 1(8):945-51. PMC: 553140. DOI: 10.1002/j.1460-2075.1982.tb01276.x. View

15.

CARROLL S, Laughon A, Thalley B . Expression, function, and regulation of the hairy segmentation protein in the Drosophila embryo. Genes Dev. 1988; 2(7):883-90. DOI: 10.1101/gad.2.7.883. View

16.

Frigerio G, Burri M, Bopp D, Baumgartner S, Noll M . Structure of the segmentation gene paired and the Drosophila PRD gene set as part of a gene network. Cell. 1986; 47(5):735-46. DOI: 10.1016/0092-8674(86)90516-7. View

17.

Parkhurst S, Corces V . Interactions among the gypsy transposable element and the yellow and the suppressor of hairy-wing loci in Drosophila melanogaster. Mol Cell Biol. 1986; 6(1):47-53. PMC: 367482. DOI: 10.1128/mcb.6.1.47-53.1986. View

18.

Dierks P, Ooyen A, Cochran M, Dobkin C, Reiser J, Weissmann C . Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit beta-globin gene in mouse 3T6 cells. Cell. 1983; 32(3):695-706. DOI: 10.1016/0092-8674(83)90055-7. View

19.

Nusslein-Volhard C, Wieschaus E . Mutations affecting segment number and polarity in Drosophila. Nature. 1980; 287(5785):795-801. DOI: 10.1038/287795a0. View

20.

Hunt T . False starts in translational control of gene expression. Nature. 1985; 316(6029):580-1. DOI: 10.1038/316580a0. View