Permeabilization of the Plasma Membrane by Deletion Mutants of Diphtheria Toxin

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1989 Oct 1

PMID 2479538

Citations 6

Authors

H Stenmark

S McGill

S Olsnes

K Sandvig

Affiliations

Soon will be listed here.

Abstract

Diphtheria toxin B-fragment binds to cell-surface receptors and facilitates translocation of the enzymatically active A-fragment to the cytosol. In this process the B-fragment inserts into the plasma membrane and induces formation of cation-selective channels. We examined the ability of a number of diphtheria toxin-derived molecules translated in vitro to permeabilize cells. Two proteins consisting of the whole B-fragment and small parts of the A-fragment, and one protein comprising most of the B-fragment alone, were more efficient than full-length toxin in permeabilizing the plasma membrane to monovalent cations. Two shorter B-fragment-derived proteins, with 3 and 10 kd N-terminal deletions, permeabilized the cells to sulfate and sucrose in addition to monovalent cations. The relationship between channel formation and toxin translocation is discussed.

Citing Articles

GPI-anchored diphtheria toxin receptor allows membrane translocation of the toxin without detectable ion channel activity.

Lanzrein M, Sand O, Olsnes S EMBO J. 1996; 15(4):725-34.

PMID: 8631294 PMC: 450271.

Protein toxins acting on intracellular targets: cellular uptake and translocation to the cytosol.

Olsnes S, van Deurs B, Sandvig K Med Microbiol Immunol. 1993; 182(2):51-61.

PMID: 8392658 DOI: 10.1007/BF00189373.

Subcloning and characterization of the binding domain of fragment B of diphtheria toxin.

Esbensen Q, Falnes P, Olsnes S, Madshus I Biochem J. 1993; 294 ( Pt 3):663-6.

PMID: 8379922 PMC: 1134513. DOI: 10.1042/bj2940663.

Diphtheria toxin endocytosis and membrane translocation are dependent on the intact membrane-anchored receptor (HB-EGF precursor): studies on the cell-associated receptor cleaved by a metalloprotease in phorbol-ester-treated cells.

Lanzrein M, Garred O, Olsnes S, Sandvig K Biochem J. 1995; 310 ( Pt 1):285-9.

PMID: 7646457 PMC: 1135885. DOI: 10.1042/bj3100285.

Membrane interactions of diphtheria toxin analyzed using in vitro synthesized mutants.

McGill S, Stenmark H, Sandvig K, Olsnes S EMBO J. 1989; 8(10):2843-8.

PMID: 2684631 PMC: 401336. DOI: 10.1002/j.1460-2075.1989.tb08431.x.

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