High-confidence Glycosome Proteome for Procyclic Form Trypanosoma Brucei by Epitope-tag Organelle Enrichment and SILAC Proteomics

Overview

Journal J Proteome Res

Publisher American Chemical Society

Specialty Biochemistry

Date 2014 May 6

PMID 24792668

Citations 57

Authors

Maria Lucia S Guther

Michael D Urbaniak

Amy Tavendale

Alan Prescott

Michael A J Ferguson

Affiliations

Soon will be listed here.

Abstract

The glycosome of the pathogenic African trypanosome Trypanosoma brucei is a specialized peroxisome that contains most of the enzymes of glycolysis and several other metabolic and catabolic pathways. The contents and transporters of this membrane-bounded organelle are of considerable interest as potential drug targets. Here we use epitope tagging, magnetic bead enrichment, and SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite using isotope ratios to discriminate glycosomal from mitochondrial and other contaminating proteins. The data confirm the presence of several previously demonstrated and suggested pathways in the organelle and identify previously unanticipated activities, such as protein phosphatases. The implications of the findings are discussed.

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