Calponin Expression in Renal Tubulointerstitial Fibrosis Induced in Rats by Cisplatin

Overview

Journal J Toxicol Pathol

Date 2014 May 3

PMID 24791074

Citations 6

Authors

Takahiro Yuasa

Ryo Yano

Takeshi Izawa

Mitsuru Kuwamura

Jyoji Yamate

Affiliations

Soon will be listed here.

Abstract

Renal tubulointerstitial fibrosis is the common feature of chronic renal failure, regardless of its etiology. Myofibroblasts play important roles in progression of the fibrosis and are characterized by expressions of various cytoskeletons such as vimentin, desmin and α-smooth muscle actin (α-SMA). To pursue the characteristics of the cells, we immunohistochemically investigated the relationship between calponin (a marker of terminal smooth muscles) expression and myofibroblasts in cisplatin-induced rat renal tubulointerstitial fibrosis. Calponin-expressing interstitial cells increased with fibrosis and reacted simultaneously to vimentin or α-SMA (a marker of well-differentiated myofibroblasts) but not desmin or Thy-1 (a marker of myofibroblasts at the early stage). The present study shows that calponin may be expressed transiently in relatively well-developed myofibroblasts in rat renal fibrosis. Calponin could become a marker for myofibroblast development in chronic renal toxicity in rats.

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References

Picard N, Baum O, Vogetseder A, Kaissling B, Le Hir M . Origin of renal myofibroblasts in the model of unilateral ureter obstruction in the rat. Histochem Cell Biol. 2008; 130(1):141-55. PMC: 2413112. DOI: 10.1007/s00418-008-0433-8. View

Lee S, Choi J, Jin D, Kim J, Cha J . Expression of calponin in periglomerular myofibroblasts of rat kidney with experimental chronic injuries. Anat Cell Biol. 2010; 43(2):132-9. PMC: 2998789. DOI: 10.5115/acb.2010.43.2.132. View

Hagood J, Miller P, Lasky J, Tousson A, Guo B, Fuller G . Differential expression of platelet-derived growth factor-alpha receptor by Thy-1(-) and Thy-1(+) lung fibroblasts. Am J Physiol. 1999; 277(1):L218-24. DOI: 10.1152/ajplung.1999.277.1.L218. View

Sappino A, Schurch W, Gabbiani G . Differentiation repertoire of fibroblastic cells: expression of cytoskeletal proteins as marker of phenotypic modulations. Lab Invest. 1990; 63(2):144-61. View

Chaurasia S, Kaur H, de Medeiros F, Smith S, Wilson S . Dynamics of the expression of intermediate filaments vimentin and desmin during myofibroblast differentiation after corneal injury. Exp Eye Res. 2009; 89(2):133-9. PMC: 2716066. DOI: 10.1016/j.exer.2009.02.022. View

Rajkumar V, Howell K, Csiszar K, Denton C, Black C, Abraham D . Shared expression of phenotypic markers in systemic sclerosis indicates a convergence of pericytes and fibroblasts to a myofibroblast lineage in fibrosis. Arthritis Res Ther. 2005; 7(5):R1113-23. PMC: 1257439. DOI: 10.1186/ar1790. View

Humphreys B, Lin S, Kobayashi A, Hudson T, Nowlin B, Bonventre J . Fate tracing reveals the pericyte and not epithelial origin of myofibroblasts in kidney fibrosis. Am J Pathol. 2009; 176(1):85-97. PMC: 2797872. DOI: 10.2353/ajpath.2010.090517. View

Nakatsuji S, Yamate J, Sakuma S . Macrophages, myofibroblasts, and extracellular matrix accumulation in interstitial fibrosis of chronic progressive nephropathy in aged rats. Vet Pathol. 1998; 35(5):352-60. DOI: 10.1177/030098589803500504. View

Rege T, Hagood J . Thy-1, a versatile modulator of signaling affecting cellular adhesion, proliferation, survival, and cytokine/growth factor responses. Biochim Biophys Acta. 2006; 1763(10):991-9. PMC: 1781924. DOI: 10.1016/j.bbamcr.2006.08.008. View

10.

Lin S, Kisseleva T, Brenner D, Duffield J . Pericytes and perivascular fibroblasts are the primary source of collagen-producing cells in obstructive fibrosis of the kidney. Am J Pathol. 2008; 173(6):1617-27. PMC: 2626374. DOI: 10.2353/ajpath.2008.080433. View

11.

Koumas L, Smith T, Feldon S, Blumberg N, Phipps R . Thy-1 expression in human fibroblast subsets defines myofibroblastic or lipofibroblastic phenotypes. Am J Pathol. 2003; 163(4):1291-300. PMC: 1868289. DOI: 10.1016/S0002-9440(10)63488-8. View

12.

Petersen B, Goff J, Greenberger J, Michalopoulos G . Hepatic oval cells express the hematopoietic stem cell marker Thy-1 in the rat. Hepatology. 1998; 27(2):433-45. DOI: 10.1002/hep.510270218. View

13.

Pei Y, Sherry D, McDermott A . Thy-1 distinguishes human corneal fibroblasts and myofibroblasts from keratocytes. Exp Eye Res. 2004; 79(5):705-12. DOI: 10.1016/j.exer.2004.08.002. View

14.

Zhou Y, Hagood J, Murphy-Ullrich J . Thy-1 expression regulates the ability of rat lung fibroblasts to activate transforming growth factor-beta in response to fibrogenic stimuli. Am J Pathol. 2004; 165(2):659-69. PMC: 1618578. DOI: 10.1016/s0002-9440(10)63330-5. View

15.

Juniantito V, Izawa T, Yuasa T, Ichikawa C, Yamamoto E, Kuwamura M . Immunophenotypical analyses of myofibroblasts in rat excisional wound healing: possible transdifferentiation of blood vessel pericytes and perifollicular dermal sheath cells into myofibroblasts. Histol Histopathol. 2012; 27(4):515-27. DOI: 10.14670/HH-27.515. View

16.

Zeisberg M, Kalluri R . The role of epithelial-to-mesenchymal transition in renal fibrosis. J Mol Med (Berl). 2004; 82(3):175-81. DOI: 10.1007/s00109-003-0517-9. View

17.

Gimona M, Kaverina I, Resch G, Vignal E, Burgstaller G . Calponin repeats regulate actin filament stability and formation of podosomes in smooth muscle cells. Mol Biol Cell. 2003; 14(6):2482-91. PMC: 194896. DOI: 10.1091/mbc.e02-11-0743. View

18.

Schauer I, Ressler S, Tuxhorn J, Dang T, Rowley D . Elevated epithelial expression of interleukin-8 correlates with myofibroblast reactive stroma in benign prostatic hyperplasia. Urology. 2008; 72(1):205-13. PMC: 2528841. DOI: 10.1016/j.urology.2007.11.083. View

19.

Yuasa T, Izawa T, Kuwamura M, Yamate J . Thy-1 Expressing Mesenchymal Cells in Rat Nephrogenesis in Correlation with Cells Immunoreactive for α-Smooth Muscle Actin and Vimentin. J Toxicol Pathol. 2012; 23(1):1-10. PMC: 3234650. DOI: 10.1293/tox.23.1. View

20.

Hewitson T . Renal tubulointerstitial fibrosis: common but never simple. Am J Physiol Renal Physiol. 2009; 296(6):F1239-44. DOI: 10.1152/ajprenal.90521.2008. View