Comparative Analysis Reveals Similarities Between Cultured Submandibular Salivary Gland Cells and Liver Progenitor Cells

Overview

Journal Springerplus

Date 2014 May 3

PMID 24790827

Citations 5

Authors

Olga S Petrakova

Vasiliy V Terskikh

Elena S Chernioglo

Vasiliy V Ashapkin

Evgeny Y Bragin

Victoria Y Shtratnikova

Inessa G Gvazava

Yuriy V Sukhanov

Andrey V Vasiliev

Affiliations

Soon will be listed here.

Abstract

Mouse submandibular salivary gland cells and liver progenitor cells from long-term in vitro cultures with a high proliferation potential were side-by-side compared by methods of immunocytochemistry, quantitative real-time PCR, flow cytometry, and transcriptome analysis. The two cell types were found to be similar in expressing cell markers such as EpCAM, CD29, c-Kit, Sca-1, and c-Met. In addition, both cell types expressed cytokeratins 8, 18, and 19, alpha-fetoprotein, and (weakly) albumin. Unlike the liver cells, however, the salivary gland cells in culture showed high-level expression of cytokeratin 14 and CD49f, which was indicative of their origin from salivary gland ducts. Quantitative real-time PCR and deep-sequencing transcriptome analysis revealed similarities in the expression pattern of transcription factors between the two cell types. In this respect, however, the cultured salivary gland cells proved to be closer to exocrine cells of the pancreas than to the liver progenitor cells. Thus, ductal cells of postnatal submandibular salivary glands in culture show phenotypic convergence with progenitor cells of endodermal origin, suggesting that these glands may serve as a potential cell source for cellular therapy of hepatic and pancreatic disorders. The results of this study provide a deeper insight into the molecular features of salivary gland cells and may help optimize procedures for stimulating their differentiation in a specified direction.

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References

Katsumoto K, Kume S . Endoderm and mesoderm reciprocal signaling mediated by CXCL12 and CXCR4 regulates the migration of angioblasts and establishes the pancreatic fate. Development. 2011; 138(10):1947-55. DOI: 10.1242/dev.058719. View

Rothova M, Thompson H, Lickert H, Tucker A . Lineage tracing of the endoderm during oral development. Dev Dyn. 2012; 241(7):1183-91. DOI: 10.1002/dvdy.23804. View

Hay D, Fletcher J, Payne C, Terrace J, Gallagher R, Snoeys J . Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling. Proc Natl Acad Sci U S A. 2008; 105(34):12301-6. PMC: 2518825. DOI: 10.1073/pnas.0806522105. View

Cayo M, Cai J, DeLaForest A, Noto F, Nagaoka M, Clark B . JD induced pluripotent stem cell-derived hepatocytes faithfully recapitulate the pathophysiology of familial hypercholesterolemia. Hepatology. 2012; 56(6):2163-71. PMC: 3900031. DOI: 10.1002/hep.25871. View

Yi F, Liu G, Izpisua Belmonte J . Rejuvenating liver and pancreas through cell transdifferentiation. Cell Res. 2012; 22(4):616-9. PMC: 3317567. DOI: 10.1038/cr.2012.33. View

Sato A, Okumura K, Matsumoto S, Hattori K, Hattori S, Shinohara M . Isolation, tissue localization, and cellular characterization of progenitors derived from adult human salivary glands. Cloning Stem Cells. 2007; 9(2):191-205. DOI: 10.1089/clo.2006.0054. View

Okumura K, Nakamura K, Hisatomi Y, Nagano K, Tanaka Y, Terada K . Salivary gland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineages. Hepatology. 2003; 38(1):104-13. DOI: 10.1053/jhep.2003.50259. View

Tsuji T, Nagai N . Production of alpha-fetoprotein by human submandibular gland. Int J Dev Biol. 1993; 37(3):497-8. View

Hisatomi Y, Okumura K, Nakamura K, Matsumoto S, Satoh A, Nagano K . Flow cytometric isolation of endodermal progenitors from mouse salivary gland differentiate into hepatic and pancreatic lineages. Hepatology. 2004; 39(3):667-75. DOI: 10.1002/hep.20063. View

10.

Trzpis M, McLaughlin P, de Leij L, Harmsen M . Epithelial cell adhesion molecule: more than a carcinoma marker and adhesion molecule. Am J Pathol. 2007; 171(2):386-95. PMC: 1934518. DOI: 10.2353/ajpath.2007.070152. View

11.

Su N, Thiaville M, Awad K, Gjymishka A, Brant J, Yang T . Protein or amino acid deprivation differentially regulates the hepatic forkhead box protein A (FOXA) genes through an activating transcription factor-4-independent pathway. Hepatology. 2009; 50(1):282-90. PMC: 3594789. DOI: 10.1002/hep.22971. View

12.

Mizumoto H, Aoki K, Nakazawa K, Ijima H, Funatsu K, Kajiwara T . Hepatic differentiation of embryonic stem cells in HF/organoid culture. Transplant Proc. 2008; 40(2):611-3. DOI: 10.1016/j.transproceed.2008.01.023. View

13.

Soto-Gutierrez A, Navarro-Alvarez N, Caballero-Corbalan J, Tanaka N, Kobayashi N . Endoderm induction for hepatic and pancreatic differentiation of ES cells. Acta Med Okayama. 2008; 62(2):63-8. DOI: 10.18926/AMO/30961. View

14.

Rambhatla L, Chiu C, Kundu P, Peng Y, Carpenter M . Generation of hepatocyte-like cells from human embryonic stem cells. Cell Transplant. 2003; 12(1):1-11. DOI: 10.3727/000000003783985179. View

15.

Murry C, Keller G . Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell. 2008; 132(4):661-80. DOI: 10.1016/j.cell.2008.02.008. View

16.

Baek H, Noh Y, Lee J, Yeon S, Jeong J, Kwon H . Autonomous isolation, long-term culture and differentiation potential of adult salivary gland-derived stem/progenitor cells. J Tissue Eng Regen Med. 2012; 8(9):717-27. DOI: 10.1002/term.1572. View

17.

Zaret K, Grompe M . Generation and regeneration of cells of the liver and pancreas. Science. 2008; 322(5907):1490-4. PMC: 2641009. DOI: 10.1126/science.1161431. View

18.

Huch M, Dorrell C, Boj S, van Es J, Li V, van de Wetering M . In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature. 2013; 494(7436):247-50. PMC: 3634804. DOI: 10.1038/nature11826. View

19.

Egea J, Hirtz C, Gross R, Lajoix A, Traskawka E, Ribes G . Preproinsulin I and II mRNA expression in adult rat submandibular glands. Eur J Oral Sci. 2000; 108(4):292-6. DOI: 10.1034/j.1600-0722.2000.108004292.x. View

20.

Bisgaard H, Thorgeirsson S . Evidence for a common cell of origin for primitive epithelial cells isolated from rat liver and pancreas. J Cell Physiol. 1991; 147(2):333-43. DOI: 10.1002/jcp.1041470220. View