Ultrastructural Localization of Polygalacturonase in Ethylene-stimulated Abscission of Tomato Pedicel Explants

Overview

Journal ScientificWorldJournal

Publisher Wiley

Specialty Biology

Date 2014 May 3

PMID 24790564

Citations 6

Authors

Ming-Fang Qi

Tao Xu

Wei-Zhi Chen

Tian-Lai Li

Affiliations

Soon will be listed here.

Abstract

Polygalacturonase (PG) is crucial in plant organ abscission process. This paper investigated the cellular and subcellular localization of PG in ethylene-stimulated abscission of tomato pedicel explants. Confocal laser scanning microscopy of abscission zone sections with the fluorescent probe Cy3 revealed that PG was initially accumulated in parenchyma cells in cortical and vascular tissues after 8 h of ethylene treatment and then extended throughout the abscission zone when the abscission zone separated at 24 h after ethylene treatment. At the subcellular level, transmission electron microscopy with immunogold staining showed that PG showed abundant accumulation in the cortical and vascular tissues at 8 h after ethylene treatment, and the distribution area extended to the central parenchyma cells at 16 h after ethylene treatment. In addition, PGs were observed in the distal and proximal parts of the tomato pedicel explants throughout the abscission process. The results provided a visualized distribution of PG in the pedicel abscission zone and proved that PG was closely related to abscission.

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References

Kalaitzis P, Solomos T, Tucker M . Three different polygalacturonases are expressed in tomato leaf and flower abscission, each with a different temporal expression pattern. Plant Physiol. 1997; 113(4):1303-8. PMC: 158253. DOI: 10.1104/pp.113.4.1303. View

Bar-Dror T, Dermastia M, Kladnik A, Znidaric M, Novak M, Meir S . Programmed cell death occurs asymmetrically during abscission in tomato. Plant Cell. 2011; 23(11):4146-63. PMC: 3246325. DOI: 10.1105/tpc.111.092494. View

Gonzalez-Carranza Z, Elliott K, Roberts J . Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana. J Exp Bot. 2007; 58(13):3719-30. DOI: 10.1093/jxb/erm222. View

Atkinson R, Schroder R, Hallett I, Cohen D, MacRae E . Overexpression of polygalacturonase in transgenic apple trees leads to a range of novel phenotypes involving changes in cell adhesion. Plant Physiol. 2002; 129(1):122-33. PMC: 155877. DOI: 10.1104/pp.010986. View

Sander L, Child R, Ulvskov P, Albrechtsen M, Borkhardt B . Analysis of a dehiscence zone endo-polygalacturonase in oilseed rape (Brassica napus) and Arabidopsis thaliana: evidence for roles in cell separation in dehiscence and abscission zones, and in stylar tissues during pollen tube growth. Plant Mol Biol. 2001; 46(4):469-79. DOI: 10.1023/a:1010619002833. View

Faulk W, Taylor G . An immunocolloid method for the electron microscope. Immunochemistry. 1971; 8(11):1081-3. DOI: 10.1016/0019-2791(71)90496-4. View

Kim J, Patterson S . Expression divergence and functional redundancy of polygalacturonases in floral organ abscission. Plant Signal Behav. 2009; 1(6):281-3. PMC: 2634239. DOI: 10.4161/psb.1.6.3541. View

Liljegren S . Organ abscission: exit strategies require signals and moving traffic. Curr Opin Plant Biol. 2012; 15(6):670-6. DOI: 10.1016/j.pbi.2012.09.012. View

Asif M, Nath P . Expression of multiple forms of polygalacturonase gene during ripening in banana fruit. Plant Physiol Biochem. 2005; 43(2):177-84. DOI: 10.1016/j.plaphy.2005.01.011. View

10.

Wang X, Liu D, Li A, Sun X, Zhang R, Wu L . Transcriptome analysis of tomato flower pedicel tissues reveals abscission zone-specific modulation of key meristem activity genes. PLoS One. 2013; 8(2):e55238. PMC: 3563536. DOI: 10.1371/journal.pone.0055238. View

11.

Jiang C, Lu F, Imsabai W, Meir S, Reid M . Silencing polygalacturonase expression inhibits tomato petiole abscission. J Exp Bot. 2008; 59(4):973-9. DOI: 10.1093/jxb/ern023. View

12.

Whitelaw C, Swarup R, Roberts J . Temporal and spatial expression of a polygalacturonase during leaf and flower abscission in oilseed rape and Arabidopsis. Plant Physiol. 2002; 128(2):534-43. PMC: 148916. DOI: 10.1104/pp.010610. View

13.

Rubinstein B, Leopold A . Analysis of the Auxin Control of Bean Leaf Abscission. Plant Physiol. 1963; 38(3):262-7. PMC: 549916. DOI: 10.1104/pp.38.3.262. View

14.

Thompson D, Osborne D . A Role for the Stele in Intertissue Signaling in the Initiation of Abscission in Bean Leaves (Phaseolus vulgaris L.). Plant Physiol. 1994; 105(1):341-347. PMC: 159362. DOI: 10.1104/pp.105.1.341. View

15.

Berger R, Reid P . Role of polygalacturonase in bean leaf abscission. Plant Physiol. 1979; 63(6):1133-7. PMC: 542983. DOI: 10.1104/pp.63.6.1133. View

16.

Iwai H, Terao A, Satoh S . Changes in distribution of cell wall polysaccharides in floral and fruit abscission zones during fruit development in tomato (Solanum lycopersicum). J Plant Res. 2012; 126(3):427-37. DOI: 10.1007/s10265-012-0536-0. View

17.

Trainotti L, Rascio N, Casadoro G . Expression of an endopolygalacturonase gene during growth and abscission of peach fruits. Hereditas. 1993; 119(3):301-4. DOI: 10.1111/j.1601-5223.1993.00301.x. View

18.

Geoghegan W, Ackerman G . Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a.... J Histochem Cytochem. 1977; 25(11):1187-200. DOI: 10.1177/25.11.21217. View

19.

Gonzalez-Carranza Z, Shahid A, Zhang L, Liu Y, Ninsuwan U, Roberts J . A novel approach to dissect the abscission process in Arabidopsis. Plant Physiol. 2012; 160(3):1342-56. PMC: 3490599. DOI: 10.1104/pp.112.205955. View

20.

Holgate C, Jackson P, Cowen P, Bird C . Immunogold-silver staining: new method of immunostaining with enhanced sensitivity. J Histochem Cytochem. 1983; 31(7):938-44. DOI: 10.1177/31.7.6189883. View