Inhibition of PaCaMKII-E Isoform in the Dorsal Unpaired Median Neurosecretory Cells of Cockroach Reduces Nicotine- and Clothianidin-induced Currents
Overview
Authors
Affiliations
Cellular responses to Ca(2+) require intermediary proteins such as calcium/calmodulin-dependent protein kinase II (CaMKII), which transduces the signal into downstream effects. We recently demonstrated that the cockroach genome encodes five different CaMKII isoforms, and only PaCaMKII-E isoform is specifically expressed in the dorsal unpaired median neurosecretory cells. In the present study, using antisense oligonucleotides, we demonstrated that PaCaMKII-E isoform inhibition reduced nicotine-induced currents through α-bungarotoxin-sensitive and -insensitive nicotinic acetylcholine receptor subtypes. Specifically, PaCaMKII-E isoform is sufficient to repress nicotinic current amplitudes as a result of its depression by antisense oligonucleotides. Similar results were found using the neonicotinoid insecticide clothianidin, which acted as a full agonist of dorsal unpaired median neuron nicotinic acetylcholine receptors. Clothianidin current amplitudes are strongly reduced under bath application of PaCaMKII-E antisense oligonucleotides but no significant results are found with α-bungarotoxin co-applied, demonstrating that CaMKII-E isoform affects nicotine currents through α-bungarotoxin-sensitive and -insensitive receptor subtypes whereas clothianidin currents are reduced via α-bungarotoxin-insensitive receptors. In addition, we found that intracellular calcium increase induced by nicotine and clothianidin were reduced by PaCaMKII-E antisense oligonucleotides, demonstrating that intracellular calcium increase induced by nicotine and clothianidin are affected by PaCaMKII-E inhibition. Cellular responses to Ca(2+) require intermediary proteins such as calcium/calmodulin-dependent protein kinase II (CaMKII). We recently demonstrated that the cockroach genome encodes five different CaMKII isoforms and only PaCaMKII-E isoform was specifically expressed in the dorsal unpaired median neurosecretory cells. Here we show that specific inhibition of PaCaMKII-E isoform is associated with a decrease in nicotine- and clothianidin-induced currents. In addition, analysis of calcium changes demonstrates that PaCaMKII-E inhibition induces a decrease in intracellular calcium concentration.
Can Virus-like Particles Be Used as Synergistic Agent in Pest Management?.
Deshayes C, Gosselin-Grenet A, Ogliastro M, Lapied B, Apaire-Marchais V Viruses. 2022; 14(5).
PMID: 35632685 PMC: 9144638. DOI: 10.3390/v14050943.
Zoh M, Bonneville J, Tutagata J, Laporte F, Fodjo B, Mouhamadou C Sci Rep. 2021; 11(1):19501.
PMID: 34593941 PMC: 8484614. DOI: 10.1038/s41598-021-99061-x.
Perrier S, Moreau E, Deshayes C, El-Adouzi M, Goven D, Chandre F Commun Biol. 2021; 4(1):665.
PMID: 34079061 PMC: 8172894. DOI: 10.1038/s42003-021-02192-0.