Human Immunodeficiency Virus 1 Tat Protein Binds Trans-activation-responsive Region (TAR) RNA in Vitro

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1989 Sep 1

PMID 2476805

Citations 219

Authors

C Dingwall

I Ernberg

M J Gait

S M Green

S Heaphy

J Karn

A D Lowe

M Singh

M A Skinner

R Valerio

Affiliations

Soon will be listed here.

Abstract

tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.

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