Alternative Splicing Contributes to the Coordinated Regulation of Ferritin Subunit Levels in Bactrocera Dorsalis (Hendel)

Overview

Journal Sci Rep

Specialty Science

Date 2014 Apr 26

PMID 24763285

Citations 8

Authors

Xuan-Zhao Jiang

Lin Cong

Jin-Zhi Niu

Wei Dou

Jin-Jun Wang

Affiliations

Soon will be listed here.

Abstract

A constant ratio of ferritin heavy chain homolog (HCH) and light chain homolog (LCH) subunits seems to be required to compose the ferritin heteropolymer protein in insects. However, the mechanism by which insect LCH genes regulate protein levels remains unclear. We report that alternative promoters and alternative splicing contribute to maintaining a constant ratio of the two subunits, BdFer1HCH and BdFer2LCH (ferritin 1 HCH and ferritin 2 LCH), in Bactrocera dorsalis, a notorious quarantine pest. The genes BdFer1HCH and BdFer2LCH were identified with a series of potential transcription factor binding sites and were shown to be clustered within the genome in a "head to head" fashion. Thus, we unearthed a potential post-transcriptional mechanism to regulate the levels of LCH subunits, and confirmed that the expressions of BdFer1HCH and BdFer2LCH were induced by 20-hydroecdysone, iron overload, and immune challenge.

Citing Articles

Iron homeostasis and organismal aging.

Zeidan R, Han S, Leeuwenburgh C, Xiao R Ageing Res Rev. 2021; 72:101510.

PMID: 34767974 PMC: 8620744. DOI: 10.1016/j.arr.2021.101510.

Loss of ferritin in developing wing cells: Apoptosis and ferroptosis coincide.

Hernandez-Gallardo A, Missirlis F PLoS Genet. 2020; 16(1):e1008503.

PMID: 31895941 PMC: 6939899. DOI: 10.1371/journal.pgen.1008503.

Characterization and Function of Two Short Peptidoglycan Recognition Proteins Involved in the Immunity of (Hendel).

Wei D, Liu Y, Zhang Y, Wang J Insects. 2019; 10(3).

PMID: 30893923 PMC: 6468497. DOI: 10.3390/insects10030079.

Antimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel).

Liu S, Li H, Yang Y, Wei D, Jiang H, Dou W AMB Express. 2018; 8(1):5.

PMID: 29327267 PMC: 5764898. DOI: 10.1186/s13568-017-0533-8.

De novo Transcriptome Analysis of Chinese Citrus Fly, Bactrocera minax (Diptera: Tephritidae), by High-Throughput Illumina Sequencing.

Wang J, Xiong K, Liu Y PLoS One. 2016; 11(6):e0157656.

PMID: 27331903 PMC: 4917245. DOI: 10.1371/journal.pone.0157656.

References

Nappi A, Vass E . Iron, metalloenzymes and cytotoxic reactions. Cell Mol Biol (Noisy-le-grand). 2000; 46(3):637-47. View

Paskewitz S, Shi L . The hemolymph proteome of Anopheles gambiae. Insect Biochem Mol Biol. 2005; 35(8):815-24. DOI: 10.1016/j.ibmb.2005.03.002. View

Yoshiga T, Georgieva T, Dunkov B, Harizanova N, Ralchev K, Law J . Drosophila melanogaster transferrin. Cloning, deduced protein sequence, expression during the life cycle, gene localization and up-regulation on bacterial infection. Eur J Biochem. 1999; 260(2):414-20. DOI: 10.1046/j.1432-1327.1999.00173.x. View

Geiser D, Chavez C, Flores-Munguia R, Winzerling J, Pham D . Aedes aegypti ferritin. Eur J Biochem. 2003; 270(18):3667-74. DOI: 10.1046/j.1432-1033.2003.03709.x. View

Hentze M, Kuhn L . Molecular control of vertebrate iron metabolism: mRNA-based regulatory circuits operated by iron, nitric oxide, and oxidative stress. Proc Natl Acad Sci U S A. 1996; 93(16):8175-82. PMC: 38642. DOI: 10.1073/pnas.93.16.8175. View

Clarke A, Armstrong K, Carmichael A, Milne J, Raghu S, Roderick G . Invasive phytophagous pests arising through a recent tropical evolutionary radiation: the Bactrocera dorsalis complex of fruit flies. Annu Rev Entomol. 2004; 50:293-319. DOI: 10.1146/annurev.ento.50.071803.130428. View

Dunkov B, Georgieva T . Insect iron binding proteins: insights from the genomes. Insect Biochem Mol Biol. 2006; 36(4):300-9. DOI: 10.1016/j.ibmb.2006.01.007. View

Silver N, Best S, Jiang J, Thein S . Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol. 2006; 7:33. PMC: 1609175. DOI: 10.1186/1471-2199-7-33. View

Shen G, Jiang H, Wang X, Wang J . Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae). BMC Mol Biol. 2010; 11:76. PMC: 2972281. DOI: 10.1186/1471-2199-11-76. View

10.

Colinet H, Overgaard J, Com E, Sorensen J . Proteomic profiling of thermal acclimation in Drosophila melanogaster. Insect Biochem Mol Biol. 2013; 43(4):352-65. DOI: 10.1016/j.ibmb.2013.01.006. View

11.

Lind M, Ekengren S, Melefors O, Soderhall K . Drosophila ferritin mRNA: alternative RNA splicing regulates the presence of the iron-responsive element. FEBS Lett. 1998; 436(3):476-82. DOI: 10.1016/s0014-5793(98)01186-7. View

12.

Andersen C, Jensen J, Orntoft T . Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004; 64(15):5245-50. DOI: 10.1158/0008-5472.CAN-04-0496. View

13.

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034. PMC: 126239. DOI: 10.1186/gb-2002-3-7-research0034. View

14.

Lemaitre B, Hoffmann J . The host defense of Drosophila melanogaster. Annu Rev Immunol. 2007; 25:697-743. DOI: 10.1146/annurev.immunol.25.022106.141615. View

15.

Piulachs M, Pagone V, Belles X . Key roles of the Broad-Complex gene in insect embryogenesis. Insect Biochem Mol Biol. 2010; 40(6):468-75. DOI: 10.1016/j.ibmb.2010.04.006. View

16.

Reinking J, Lam M, Pardee K, Sampson H, Liu S, Yang P . The Drosophila nuclear receptor e75 contains heme and is gas responsive. Cell. 2005; 122(2):195-207. DOI: 10.1016/j.cell.2005.07.005. View

17.

Hamburger A, West Jr A, Hamburger Z, Hamburger P, Bjorkman P . Crystal structure of a secreted insect ferritin reveals a symmetrical arrangement of heavy and light chains. J Mol Biol. 2005; 349(3):558-69. DOI: 10.1016/j.jmb.2005.03.074. View

18.

Kell D . Iron behaving badly: inappropriate iron chelation as a major contributor to the aetiology of vascular and other progressive inflammatory and degenerative diseases. BMC Med Genomics. 2009; 2:2. PMC: 2672098. DOI: 10.1186/1755-8794-2-2. View

19.

Mahadav A, Kontsedalov S, Czosnek H, Ghanim M . Thermotolerance and gene expression following heat stress in the whitefly Bemisia tabaci B and Q biotypes. Insect Biochem Mol Biol. 2009; 39(10):668-76. DOI: 10.1016/j.ibmb.2009.08.002. View

20.

Shen G, Wang X, Dou W, Wang J . Biochemical and molecular characterisation of acetylcholinesterase in four field populations of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae). Pest Manag Sci. 2012; 68(12):1553-63. DOI: 10.1002/ps.3340. View