In Vitro Virucidal and Virustatic Properties of the Crude Extract of Cynodon Dactylon Against Porcine Reproductive and Respiratory Syndrome Virus

Overview

Journal Vet Med Int

Publisher Wiley

Specialty Veterinary Medicine

Date 2014 Apr 19

PMID 24744959

Citations 9

Authors

Kidsadagon Pringproa

Oapkun Khonghiran

Suchaya Kunanoppadol

Teerapong Potha

Phongsakorn Chuammitri

Affiliations

Soon will be listed here.

Abstract

The in vitro virustatic and virucidal tests of the crude extract of Cynodon dactylon against infection with porcine reproductive and respiratory syndrome virus (PRRSV), a cause of major devastating pig disease, were described. Crude extract of C. dactylon was prepared for cytotoxicity on tissue-culture cells that were used to measure virustatic and virucidal activities against PRRSV. Crude extract of C. dactylon at 0.78 mg/mL showed no cytotoxicity on the cell line, and at that concentration significantly inhibited replication of PRRSV as early as 24 hours post infection (hpi). C. dactylon also inactivated PRRSV as determined by immunoperoxidase monolayer assay (IPMA) compared to the control experiments. In summary, the present study may be among the earliest studies to describe virustatic and virucidal activities of C. dactylon crude extract against PRRSV in vitro. Extracts of C. dactylon may be useful for PRRSV control and prevention on pig farms.

Citing Articles

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References

Chand R, Trible B, Rowland R . Pathogenesis of porcine reproductive and respiratory syndrome virus. Curr Opin Virol. 2012; 2(3):256-63. DOI: 10.1016/j.coviro.2012.02.002. View

Hong J, Kim J, Jung W, Kim S, Bae W, Koo H . Prevalence and antibiotic resistance of Campylobacter spp. isolated from chicken meat, pork, and beef in Korea, from 2001 to 2006. J Food Prot. 2007; 70(4):860-6. DOI: 10.4315/0362-028x-70.4.860. View

Yang Q, Gao L, Si J, Sun Y, Liu J, Cao L . Inhibition of porcine reproductive and respiratory syndrome virus replication by flavaspidic acid AB. Antiviral Res. 2012; 97(1):66-73. DOI: 10.1016/j.antiviral.2012.11.004. View

Mendes G, Soares A, Sigiliano L, Machado F, Kaiser C, Romeiro N . In vitro anti-HMPV activity of meroditerpenoids from marine alga Stypopodium zonale (Dictyotales). Molecules. 2011; 16(10):8437-50. PMC: 6264370. DOI: 10.3390/molecules16108437. View

Corzo C, Mondaca E, Wayne S, Torremorell M, Dee S, Davies P . Control and elimination of porcine reproductive and respiratory syndrome virus. Virus Res. 2010; 154(1-2):185-92. DOI: 10.1016/j.virusres.2010.08.016. View

Singh S, Kesari A, Gupta R, Jaiswal D, Watal G . Assessment of antidiabetic potential of Cynodon dactylon extract in streptozotocin diabetic rats. J Ethnopharmacol. 2007; 114(2):174-9. DOI: 10.1016/j.jep.2007.07.039. View

Kang Y, Cho Y, Yoon S, Yu M, Kim C, Lee J . Prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from raw chicken meat and human stools in Korea. J Food Prot. 2006; 69(12):2915-23. DOI: 10.4315/0362-028x-69.12.2915. View

Shi M, Lam T, Hon C, Hui R, Faaberg K, Wennblom T . Molecular epidemiology of PRRSV: a phylogenetic perspective. Virus Res. 2010; 154(1-2):7-17. DOI: 10.1016/j.virusres.2010.08.014. View

Wei Z, Wang X, Zhang H, Yang C, Wang Y, Xu D . Inhibitory effects of indigowoad root polysaccharides on porcine reproductive and respiratory syndrome virus replication in vitro. Antivir Ther. 2011; 16(3):357-63. DOI: 10.3851/IMP1755. View

10.

Bag P, Chattopadhyay D, Mukherjee H, Ojha D, Mandal N, Sarkar M . Anti-herpes virus activities of bioactive fraction and isolated pure constituent of Mallotus peltatus: an ethnomedicine from Andaman Islands. Virol J. 2012; 9:98. PMC: 3430555. DOI: 10.1186/1743-422X-9-98. View

11.

Thanawongnuwech R, Thacker E, Halbur P . Influence of pig age on virus titer and bactericidal activity of porcine reproductive and respiratory syndrome virus (PRRSV)-infected pulmonary intravascular macrophages (PIMs). Vet Microbiol. 1998; 63(2-4):177-87. DOI: 10.1016/s0378-1135(98)00245-4. View

12.

Khajavi Rad A, Hadjzadeh M, Rajaei Z, Mohammadian N, Valiollahi S, Sonei M . The beneficial effect of cynodon dactylon fractions on ethylene glycol-induced kidney calculi in rats. Urol J. 2011; 8(3):179-84. View

13.

Cho J, Dee S, Deen J, Guedes A, Trincado C, Fano E . Evaluation of the effects of animal age, concurrent bacterial infection, and pathogenicity of porcine reproductive and respiratory syndrome virus on virus concentration in pigs. Am J Vet Res. 2006; 67(3):489-93. DOI: 10.2460/ajvr.67.3.489. View

14.

Schuhmacher A, Reichling J, Schnitzler P . Virucidal effect of peppermint oil on the enveloped viruses herpes simplex virus type 1 and type 2 in vitro. Phytomedicine. 2003; 10(6-7):504-10. DOI: 10.1078/094471103322331467. View

15.

Immanuel G, Uma R, Iyapparaj P, Citarasu T, Peter S, Michael Babu M . Dietary medicinal plant extracts improve growth, immune activity and survival of tilapia Oreochromis mossambicus. J Fish Biol. 2010; 74(7):1462-75. DOI: 10.1111/j.1095-8649.2009.02212.x. View

16.

Thanawongnuwech R, Suradhat S . Taming PRRSV: revisiting the control strategies and vaccine design. Virus Res. 2010; 154(1-2):133-40. DOI: 10.1016/j.virusres.2010.09.003. View

17.

Albina E . Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview. Vet Microbiol. 1997; 55(1-4):309-16. DOI: 10.1016/s0378-1135(96)01322-3. View

18.

Sun N, Zhao X, Bai X, Niu L, Song M, Sun Y . Anti-PRRSV effect and mechanism of sodium tanshinone IIA sulfonate in vitro. J Asian Nat Prod Res. 2012; 14(8):721-8. DOI: 10.1080/10286020.2012.685727. View

19.

Garjani A, Afrooziyan A, Nazemiyeh H, Najafi M, Kharazmkia A, Maleki-Dizaji N . Protective effects of hydroalcoholic extract from rhizomes of Cynodon dactylon (L.) Pers. on compensated right heart failure in rats. BMC Complement Altern Med. 2009; 9:28. PMC: 2734535. DOI: 10.1186/1472-6882-9-28. View

20.

Balasubramanian G, Sarathi M, Venkatesan C, Thomas J, Sahul Hameed A . Studies on the immunomodulatory effect of extract of Cyanodon dactylon in shrimp, Penaeus monodon, and its efficacy to protect the shrimp from white spot syndrome virus (WSSV). Fish Shellfish Immunol. 2008; 25(6):820-8. DOI: 10.1016/j.fsi.2008.09.002. View