DNA Methylation of the LY86 Gene is Associated with Obesity, Insulin Resistance, and Inflammation

Overview

Journal Twin Res Hum Genet

Publisher Cambridge University Press

Specialty Genetics

Date 2014 Apr 17

PMID 24735745

Citations 16

Authors

Shaoyong Su

Haidong Zhu

Xiaojing Xu

Xin Wang

Yanbin Dong

Gaston Kapuku

Frank Treiber

Bernard Gutin

Gregory Harshfield

Harold Snieder

Xiaoling Wang

Affiliations

Soon will be listed here.

Abstract

Background: Previous genome-wide association studies (GWAS) have identified a large number of genetic variants for obesity and its related traits, representing a group of potential key genes in the etiology of obesity. Emerging evidence suggests that epigenetics may play an important role in obesity. It has not been explored whether the GWAS-identified loci contribute to obesity through epigenetics (e.g., DNA (deoxyribonucleic acid) methylation) in addition to genetics.

Method: A multi-stage cross-sectional study was designed. We did a literature search and identified 117 genes discovered by GWAS for obesity and its related traits. Then we analyzed whether the methylation levels of these genes were also associated with obesity in two genome-wide methylation panels. We examined an initial panel of seven adolescent obese cases and seven age-matched lean controls, followed by a second panel of 48 adolescent obese cases and 48 age- and gender-matched lean controls. The validated CpG sites were further replicated in two independent replication panels of youth (46 vs. 46 and 230 cases vs. 413 controls, respectively) and a general population of youth, including 703 healthy subjects.

Results: One CpG site in the lymphocyte antigen 86 (LY86) gene, which showed higher methylation in the obese in both the initial (p = .009) and second genome-wide DNA methylation panel (p = .008), was further validated in both replication panels (meta p = .00016). Moreover, in the general population of youth, the methylation levels of this region were significantly correlated with adiposity indices (p ≤ .02), insulin resistance (p = .001), and inflammatory markers (p < .001).

Conclusion: By focusing on recent GWAS findings in genome-wide methylation profiles, we identified a solid association between LY86 gene DNA methylation and obesity.

Citing Articles

Ding Q, Gao Z, Chen K, Zhang Q, Hu S, Zhao L Front Immunol. 2022; 13:883410.

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Fontanella R, Scisciola L, Rizzo M, Surina S, Sardu C, Marfella R Front Cardiovasc Med. 2021; 8:768026.

PMID: 34869683 PMC: 8639875. DOI: 10.3389/fcvm.2021.768026.

Aberrant DNA Methylation Involved in Obese Women with Systemic Insulin Resistance.

Zhang S, Wang Y, Yang Y, Zheng H Open Life Sci. 2021; 13:201-207.

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Adiponectin DNA methylation in South African women with gestational diabetes mellitus: Effects of HIV infection.

Dias S, Adam S, Abrahams Y, Rheeder P, Pheiffer C PLoS One. 2021; 16(3):e0248694.

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Adiposity associated DNA methylation signatures in adolescents are related to leptin and perinatal factors.

Huang R, Melton P, Burton M, Beilin L, Clarke-Harris R, Cook E Epigenetics. 2021; 17(8):819-836.

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