Interactions Among Mitochondrial Proteins Altered in Glioblastoma

Overview

Journal J Neurooncol

Publisher Springer

Date 2014 Apr 15

PMID 24728830

Citations 42

Authors

Ruth F Deighton

Thierry Le Bihan

Sarah F Martin

Alice M J Gerth

Mailis McCulloch

Julia M Edgar

Lorraine E Kerr

Ian R Whittle

James McCulloch

Affiliations

Soon will be listed here.

Abstract

Mitochondrial dysfunction is putatively central to glioblastoma (GBM) pathophysiology but there has been no systematic analysis in GBM of the proteins which are integral to mitochondrial function. Alterations in proteins in mitochondrial enriched fractions from patients with GBM were defined with label-free liquid chromatography mass spectrometry. 256 mitochondrially-associated proteins were identified in mitochondrial enriched fractions and 117 of these mitochondrial proteins were markedly (fold-change ≥ 2) and significantly altered in GBM (p ≤ 0.05). Proteins associated with oxidative damage (including catalase, superoxide dismutase 2, peroxiredoxin 1 and peroxiredoxin 4) were increased in GBM. Protein-protein interaction analysis highlighted a reduction in multiple proteins coupled to energy metabolism (in particular respiratory chain proteins, including 23 complex-I proteins). Qualitative ultrastructural analysis in GBM with electron microscopy showed a notably higher prevalence of mitochondria with cristolysis in GBM. This study highlights the complex mitochondrial proteomic adjustments which occur in GBM pathophysiology.

Citing Articles

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