Molecular Strategies for Detecting Chromosomal Translocations in Soft Tissue Tumors (review)

Overview

Journal Int J Mol Med

Specialty Genetics

Date 2014 Apr 10

PMID 24714847

Citations 4

Authors

Margherita Cerrone

Monica Cantile

Francesca Collina

Laura Marra

Giuseppina Liguori

Renato Franco

Annarosaria De Chiara

Gerardo Botti

Affiliations

Soon will be listed here.

Abstract

Approximately one third of soft tissue tumors are characterized by chromosomal aberrations, in particular, translocations and amplifications, which appear to be highly specific. The identification of fusion transcripts not only supports the diagnosis, but provides the basis for the development of novel therapeutic strategies aimed at blocking the aberrant activity of chimeric proteins. Molecular biology, and in particular, cytogenetic and qualitative and quantitative polymerase chain reaction technologies, allow with high efficiency and specificity, the determination of specific fusion transcripts resulting from chromosomal translocations, as well as the analysis of gene amplifications. In this review, various molecular techniques that allow the identification of translocations and consequent fusion transcripts generated are discussed in the broad spectrum of soft tissue tumors.

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