Phage Nanofibers Induce Vascularized Osteogenesis in 3D Printed Bone Scaffolds

Overview

Journal Adv Mater

Date 2014 Apr 9

PMID 24711251

Citations 84

Authors

Jianglin Wang

Mingying Yang

Ye Zhu

Lin Wang

Antoni P Tomsia

Chuanbin Mao

Affiliations

Soon will be listed here.

Abstract

A virus-activated matrix is developed to overcome the challenge of forming vascularized bone tissue. It is generated by filling a 3D printed bioceramic scaffold with phage nanofibers displaying high-density RGD peptide. After it is seeded with mesenchymal stem cells (MSCs) and implanted into a bone defect, the phage nanofibers induce osteogenesis and angiogenesis by activating endothelialization and osteogenic differentiation of MSCs.

Citing Articles

Oriented Cortical-Bone-Like Silk Protein Lamellae Effectively Repair Large Segmental Bone Defects in Pigs.

Shuai Y, Yang T, Zheng M, Zheng L, Wang J, Mao C Adv Mater. 2025; 37(10):e2414543.

PMID: 39871679 PMC: 11899506. DOI: 10.1002/adma.202414543.

Advances in Bioceramics for Bone Regeneration: A Narrative Review.

Brochu B, Sturm S, Kawase De Queiroz Goncalves J, Mirsky N, Sandino A, Panthaki K Biomimetics (Basel). 2024; 9(11).

PMID: 39590262 PMC: 11592113. DOI: 10.3390/biomimetics9110690.

A Comprehensive Review on Phage Therapy and Phage-Based Drug Development.

Cui L, Watanabe S, Miyanaga K, Kiga K, Sasahara T, Aiba Y Antibiotics (Basel). 2024; 13(9).

PMID: 39335043 PMC: 11428490. DOI: 10.3390/antibiotics13090870.

Quantum dots for bone tissue engineering.

Ding N, Zhou F, Li G, Shen H, Bai L, Su J Mater Today Bio. 2024; 28:101167.

PMID: 39205871 PMC: 11350444. DOI: 10.1016/j.mtbio.2024.101167.

Murine iPSC-Loaded Scaffold Grafts Improve Bone Regeneration in Critical-Size Bone Defects.

Kessler F, Arnke K, Eggerschwiler B, Neldner Y, Marsmann S, Groninger O Int J Mol Sci. 2024; 25(10).

PMID: 38791592 PMC: 11121928. DOI: 10.3390/ijms25105555.

References

Eliceiri B, Cheresh D . Adhesion events in angiogenesis. Curr Opin Cell Biol. 2001; 13(5):563-8. DOI: 10.1016/s0955-0674(00)00252-0. View

Pasqualini R, Ruoslahti E . Organ targeting in vivo using phage display peptide libraries. Nature. 1996; 380(6572):364-6. DOI: 10.1038/380364a0. View

Street J, Bao M, deGuzman L, Bunting S, Peale Jr F, Ferrara N . Vascular endothelial growth factor stimulates bone repair by promoting angiogenesis and bone turnover. Proc Natl Acad Sci U S A. 2002; 99(15):9656-61. PMC: 124965. DOI: 10.1073/pnas.152324099. View

Zhou J, Lin H, Fang T, Li X, Dai W, Uemura T . The repair of large segmental bone defects in the rabbit with vascularized tissue engineered bone. Biomaterials. 2009; 31(6):1171-9. DOI: 10.1016/j.biomaterials.2009.10.043. View

Wang F, Cao B, Mao C . Bacteriophage Bundles with Pre-Aligned Ca Initiate the Oriented Nucleation and Growth of Hydroxylapatite. Chem Mater. 2010; 22(12):3630-3636. PMC: 2926989. DOI: 10.1021/cm902727s. View

Camenisch G, Pisabarro M, Sherman D, Kowalski J, Nagel M, Hass P . ANGPTL3 stimulates endothelial cell adhesion and migration via integrin alpha vbeta 3 and induces blood vessel formation in vivo. J Biol Chem. 2002; 277(19):17281-90. DOI: 10.1074/jbc.M109768200. View

Houmard M, Fu Q, Genet M, Saiz E, Tomsia A . On the structural, mechanical, and biodegradation properties of HA/β-TCP robocast scaffolds. J Biomed Mater Res B Appl Biomater. 2013; 101(7):1233-42. DOI: 10.1002/jbm.b.32935. View

Crisan M, Yap S, Casteilla L, Chen C, Corselli M, Park T . A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008; 3(3):301-13. DOI: 10.1016/j.stem.2008.07.003. View

Quarto R, Mastrogiacomo M, Cancedda R, Kutepov S, Mukhachev V, Lavroukov A . Repair of large bone defects with the use of autologous bone marrow stromal cells. N Engl J Med. 2001; 344(5):385-6. DOI: 10.1056/NEJM200102013440516. View

10.

Mao C, Wang F, Cao B . Controlling nanostructures of mesoporous silica fibers by supramolecular assembly of genetically modifiable bacteriophages. Angew Chem Int Ed Engl. 2012; 51(26):6411-5. PMC: 3445806. DOI: 10.1002/anie.201107824. View

11.

Ngweniform P, Abbineni G, Cao B, Mao C . Self-assembly of drug-loaded liposomes on genetically engineered target-recognizing M13 phage: a novel nanocarrier for targeted drug delivery. Small. 2009; 5(17):1963-9. DOI: 10.1002/smll.200801902. View

12.

Miranda P, Pajares A, Saiz E, Tomsia A, Guiberteau F . Mechanical properties of calcium phosphate scaffolds fabricated by robocasting. J Biomed Mater Res A. 2007; 85(1):218-27. DOI: 10.1002/jbm.a.31587. View

13.

Horwitz E, Prockop D, Fitzpatrick L, Koo W, Gordon P, Neel M . Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. Nat Med. 1999; 5(3):309-13. DOI: 10.1038/6529. View

14.

Levenberg S, Rouwkema J, Macdonald M, Garfein E, Kohane D, Darland D . Engineering vascularized skeletal muscle tissue. Nat Biotechnol. 2005; 23(7):879-84. DOI: 10.1038/nbt1109. View

15.

Naito Y, Shinoka T, Duncan D, Hibino N, Solomon D, Cleary M . Vascular tissue engineering: towards the next generation vascular grafts. Adv Drug Deliv Rev. 2011; 63(4-5):312-23. DOI: 10.1016/j.addr.2011.03.001. View

16.

Rafii S, Lyden D . Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med. 2003; 9(6):702-12. DOI: 10.1038/nm0603-702. View

17.

He T, Abbineni G, Cao B, Mao C . Nanofibrous bio-inorganic hybrid structures formed through self-assembly and oriented mineralization of genetically engineered phage nanofibers. Small. 2010; 6(20):2230-5. PMC: 3102575. DOI: 10.1002/smll.201001108. View

18.

Griffith L, Naughton G . Tissue engineering--current challenges and expanding opportunities. Science. 2002; 295(5557):1009-14. DOI: 10.1126/science.1069210. View

19.

Gandra N, Abbineni G, Qu X, Huai Y, Wang L, Mao C . Bacteriophage bionanowire as a carrier for both cancer-targeting peptides and photosensitizers and its use in selective cancer cell killing by photodynamic therapy. Small. 2012; 9(2):215-21. PMC: 3703240. DOI: 10.1002/smll.201202090. View

20.

Dabrowska K, Switala-Jelen K, Opolski A, Weber-Dabrowska B, Gorski A . Bacteriophage penetration in vertebrates. J Appl Microbiol. 2004; 98(1):7-13. DOI: 10.1111/j.1365-2672.2004.02422.x. View