A HPLC-Q-TOF-MS-based Urinary Metabolomic Approach to Identification of Potential Biomarkers of Metabolic Syndrome

Overview

Journal J Huazhong Univ Sci Technolog Med Sci

Specialty General Medicine

Date 2014 Apr 9

PMID 24710945

Citations 16

Authors

Zhi-Rui Yu

Yu Ning

Hao Yu

Nai-Jun Tang

Affiliations

Soon will be listed here.

Abstract

Metabolic syndrome (MetS) is a serious threat to public health worldwide with an increased risk of developing type 2 diabetes, cardiovascular diseases and all-cause morbidity and mortality. In this study, a urinary metabolomic approach was performed on high performance liquid chromatography quadrupole time-of-flight mass spectrometry to discriminate 36 male MetS patients and 36 sex and age matched healthy controls. Pattern recognition analyses (principal component analysis and orthogonal projections to latent structures discriminate analysis) commonly demonstrated the difference between MetS patients and no-MetS subjects. This study found 8 metabolites that showed significant changes in patients with MetS, including branch-chain and aromatic amino acids (leucine, tyrosine, phenylalanine and tryptophan), short-chain acylcanitine (tiglylcarnitine), tricarboxylic acid (TCA) cycle intermediate (cis-aconitic acid) and glucuronidated products (cortolone-3-glucuronide and tetrahydroaldosterone-3-glucuronide). The candidate biomarkers revealed in this study could be useful in providing clues for further research focusing on the in-depth investigation of the cause of and cure for MetS.

Citing Articles

Zhang Q, Huang Y, Gao S, Ding Y, Zhang H, Chang G Int J Mol Sci. 2024; 25(15).

PMID: 39126056 PMC: 11312664. DOI: 10.3390/ijms25158484.

Serum metabolomics identified specific lipid compounds which may serve as markers of disease progression in patients with Alström and Bardet-Biedl syndromes.

Jeziorny K, Pietrowska K, Sieminska J, Zmyslowska-Polakowska E, Kretowski A, Ciborowski M Front Mol Biosci. 2023; 10:1251905.

PMID: 38028552 PMC: 10657895. DOI: 10.3389/fmolb.2023.1251905.

Metabolomic Phenotype of Hepatic Steatosis and Fibrosis in Mexican Children Living with Obesity.

Garibay-Nieto N, Pedraza-Escudero K, Omana-Guzman I, Garces-Hernandez M, Villanueva-Ortega E, Flores-Torres M Medicina (Kaunas). 2023; 59(10).

PMID: 37893503 PMC: 10608521. DOI: 10.3390/medicina59101785.

The metabolomic profiling of total fat and fat distribution in a multi-cohort study of women and men.

Zheng R, Michaelsson K, Fall T, Elmstahl S, Lind L Sci Rep. 2023; 13(1):11129.

PMID: 37429905 PMC: 10333321. DOI: 10.1038/s41598-023-38318-z.

Targeted Metabolomics Revealed a Sex-Dependent Signature for Metabolic Syndrome in the Mexican Population.

Palacios-Gonzalez B, Leon-Reyes G, Rivera-Paredez B, Ibarra-Gonzalez I, Vela-Amieva M, Flores Y Nutrients. 2022; 14(18).

PMID: 36145054 PMC: 9504093. DOI: 10.3390/nu14183678.

References

Eckel R, Alberti K, Grundy S, Zimmet P . The metabolic syndrome. Lancet. 2010; 375(9710):181-3. DOI: 10.1016/S0140-6736(09)61794-3. View

Schooneman M, Vaz F, Houten S, Soeters M . Acylcarnitines: reflecting or inflicting insulin resistance?. Diabetes. 2012; 62(1):1-8. PMC: 3526046. DOI: 10.2337/db12-0466. View

Soares-da-Silva P, Vieira-Coelho M, Pestana M, Bertorello A . Studies on the nature of the antagonistic actions of dopamine and 5-hydroxytryptamine in renal tissues. Hypertens Res. 1995; 18 Suppl 1:S47-51. DOI: 10.1291/hypres.18.supplementi_s47. View

Adams S . Emerging perspectives on essential amino acid metabolism in obesity and the insulin-resistant state. Adv Nutr. 2012; 2(6):445-56. PMC: 3226382. DOI: 10.3945/an.111.000737. View

Nicholson J, Wilson I . Opinion: understanding 'global' systems biology: metabonomics and the continuum of metabolism. Nat Rev Drug Discov. 2003; 2(8):668-76. DOI: 10.1038/nrd1157. View

Bijlsma S, Bobeldijk I, Verheij E, Ramaker R, Kochhar S, Macdonald I . Large-scale human metabolomics studies: a strategy for data (pre-) processing and validation. Anal Chem. 2006; 78(2):567-74. DOI: 10.1021/ac051495j. View

Guan H, Goldstein J, Brown M, Liang G . Accelerated fatty acid oxidation in muscle averts fasting-induced hepatic steatosis in SJL/J mice. J Biol Chem. 2009; 284(36):24644-52. PMC: 2782053. DOI: 10.1074/jbc.M109.034397. View

Hirose M, Tomoda F, Koike T, Yamazaki H, Ohara M, Liu H . Imbalance of renal production between 5-hydroxytryptamine and dopamine in patients with essential hypertension complicated by microalbuminuria. Am J Hypertens. 2013; 26(2):227-33. DOI: 10.1093/ajh/hps008. View

Gu D, Reynolds K, Wu X, Chen J, Duan X, Reynolds R . Prevalence of the metabolic syndrome and overweight among adults in China. Lancet. 2005; 365(9468):1398-405. DOI: 10.1016/S0140-6736(05)66375-1. View

10.

Esteghamati A, Zandieh A, Zandieh B, Khalilzadeh O, Meysamie A, Nakhjavani M . Leptin cut-off values for determination of metabolic syndrome: third national surveillance of risk factors of non-communicable diseases in Iran (SuRFNCD-2007). Endocrine. 2011; 40(1):117-23. DOI: 10.1007/s12020-011-9447-4. View

11.

Othman A, Rutti M, Ernst D, Saely C, Rein P, Drexel H . Plasma deoxysphingolipids: a novel class of biomarkers for the metabolic syndrome?. Diabetologia. 2011; 55(2):421-31. DOI: 10.1007/s00125-011-2384-1. View

12.

Zhang T, Wu X, Ke C, Yin M, Li Z, Fan L . Identification of potential biomarkers for ovarian cancer by urinary metabolomic profiling. J Proteome Res. 2012; 12(1):505-12. DOI: 10.1021/pr3009572. View

13.

Patti M, Brambilla E, Luzi L, Landaker E, Kahn C . Bidirectional modulation of insulin action by amino acids. J Clin Invest. 1998; 101(7):1519-29. PMC: 508730. DOI: 10.1172/JCI1326. View

14.

Satapati S, Sunny N, Kucejova B, Fu X, He T, Mendez-Lucas A . Elevated TCA cycle function in the pathology of diet-induced hepatic insulin resistance and fatty liver. J Lipid Res. 2012; 53(6):1080-92. PMC: 3351815. DOI: 10.1194/jlr.M023382. View

15.

Mihalik S, Goodpaster B, Kelley D, Chace D, Vockley J, Toledo F . Increased levels of plasma acylcarnitines in obesity and type 2 diabetes and identification of a marker of glucolipotoxicity. Obesity (Silver Spring). 2010; 18(9):1695-700. PMC: 3984458. DOI: 10.1038/oby.2009.510. View

16.

Zhu Z, Wang H, Shang Q, Jiang Y, Cao Y, Chai Y . Time course analysis of Candida albicans metabolites during biofilm development. J Proteome Res. 2012; 12(6):2375-85. DOI: 10.1021/pr300447k. View

17.

Alberti K, Zimmet P, Shaw J . The metabolic syndrome--a new worldwide definition. Lancet. 2005; 366(9491):1059-62. DOI: 10.1016/S0140-6736(05)67402-8. View

18.

Xu J, Kulkarni S, Li L, Slitt A . UDP-glucuronosyltransferase expression in mouse liver is increased in obesity- and fasting-induced steatosis. Drug Metab Dispos. 2011; 40(2):259-66. PMC: 3263941. DOI: 10.1124/dmd.111.039925. View

19.

Gami A, Witt B, Howard D, Erwin P, Gami L, Somers V . Metabolic syndrome and risk of incident cardiovascular events and death: a systematic review and meta-analysis of longitudinal studies. J Am Coll Cardiol. 2007; 49(4):403-14. DOI: 10.1016/j.jacc.2006.09.032. View

20.

Ong E, Zou L, Li S, Cheah P, Eu K, Ong C . Metabolic profiling in colorectal cancer reveals signature metabolic shifts during tumorigenesis. Mol Cell Proteomics. 2010; . DOI: 10.1074/mcp.M900551-MCP200. View